A5084016819- Advanced Fluorescence Microscopy Techniques
- Click Chemistry and Applications
- Photoreceptor and optogenetics research
- Luminescence and Fluorescent Materials
- Catalytic C–H Functionalization Methods
- bioluminescence and chemiluminescence research
- Synthesis and Biological Evaluation
- Synthesis and Reactions of Organic Compounds
- Photochemistry and Electron Transfer Studies
- Molecular Sensors and Ion Detection
- Synthesis and biological activity
- Synthesis of heterocyclic compounds
- Synthesis and Reactivity of Heterocycles
- Crystallization and Solubility Studies
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- Asymmetric Synthesis and Catalysis
- Chemical synthesis and alkaloids
- Photosynthetic Processes and Mechanisms
- Fluorine in Organic Chemistry
- X-ray Diffraction in Crystallography
- Sulfur-Based Synthesis Techniques
- Photodynamic Therapy Research Studies
- Chemical Synthesis and Analysis
Institute of Bioorganic Chemistry
2015-2024
Pirogov Russian National Research Medical University
2015-2024
Institute of Bioorganic Chemistry
2020-2024
Ministry of Health of the Russian Federation
2022
Russian Academy of Sciences
2015-2021
Samara State Technical University
2011-2020
Lomonosov Moscow State University
2012-2017
Peoples' Friendship University of Russia
2016
Moscow State University
2013
Study of fungal bioluminescence mechanisms generates development a multicolor enzymatic chemiluminescence system.
Abstract A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These are characterized by red‐shifted spectra, high fluorescence quantum yields and pH‐independence in physiological pH range. The intra‐ intermolecular mechanisms radiationless deactivation ABDI‐BF2 fluorophore selective structural locking various conformational degrees freedom were studied. unique combination solvatochromic lipophilic properties together with “infinite” photostability...
A genetically encoded fluorescent tag for live cell microscopy is presented. This composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative green (GFP)-like chromophore with red fluorescence. The reversible binding the fluorogen accompanied by three orders magnitude increase in fluorescence (580-650 nm). proposed dye instantly stains target cellular proteins fused FAST, washes out minute timescale, exhibits higher photostability signal confocal...
NanoFAST is the smallest fluorogen-activating protein, consisting of only 98 amino acids, used as a genetically encoded fluorescent tag. Previously, single fluorogen with an orange color was revealed for this protein. In present paper, using rational mutagenesis and in vitro screening fluorogens libraries, we expanded palette We discovered that E46Q one key substitutions enabling range possible to be expanded. The introduction several other has made it use not but also red green modified
Abstract A novel class of fluorescent dyes based on the conformationally locked heterocyclic core chromophore protein Kaede was discovered. Introduction a single conformational lock at benzylidene fragment resulted in an increase fluorescence quantum yield (FQY) by one order magnitude and redshift ca. 60 nm emission spectrum. Imposing second ethylene provided further FQY. Locked analogues demonstrated bright redshifted broad range solvents, which makes them good candidates for wide spectrum...
Superphotoacidity involves ultrafast proton motions implicated in numerous chemical and biological processes. We used conformational locking strategic addition of electron-withdrawing substituents to synthesize a new GFP chromophore analogue: p-HO-3,5-diF-BDI:BF2 (diF). It is highly fluorescent exhibits excited-state transfer (ESPT) various solvents, placing it among the strongest photoacids. Tunable femtosecond stimulated Raman spectroscopy with unique resonance conditions transient...
Novel fluorogenic dyes based on the GFP chromophore are developed. The compounds contain a pyridinium ring instead of phenolate and feature large Stokes shifts solvent-dependent variations in fluorescence quantum yield. Electronic structure calculations explain trends solvatochromic behavior terms increase dipole moment upon excited-state relaxation polar solvents associated with changes bonding pattern excited state. A unique combination such optical characteristics lipophilic properties...
We strategically modified the GFP core via chemical synthesis to make redder and brighter biomimetic fluorophores. Based on quantum calculations, solvatochromism analysis, femtosecond Raman, we unveiled additive effect of tuning electronic ground excited states, respectively, achieve a dramatic emission redshift with "double-donor-one-acceptor" structure.
One of the essential characteristics any tag used in bioscience and medical applications is its size. The larger label, more it may affect studied object, distort behavior. In this paper, using NMR spectroscopy X-ray crystallography, we have structure fluorogen-activating protein FAST both apo form complex with fluorogen. We showed that significant change occurs upon interaction ligand. While completely ordered complex, characterized by higher mobility disordering N-terminus. structural...
Proton transfer remains one of the most fundamental processes in chemistry and biology. Superphotoacids provide an excellent platform to delineate excited-state proton (ESPT) mechanism on ultrafast time scales enable precisely control photoacidity other pertinent functionalities such as fluorescence. We modified GFP core ( p-HBDI chromophore) into two series highly fluorescent photoacids by fluorinating phenolic ring conformationally locking backbone (i.e., biomimetics). The trifluorinated...
Abstract Using benzylidene imidazolone core, we created a panel of color‐shifted fluorogenic ligands for FAST protein without compromise to the binding efficiency and utility live‐cell labeling. This study highlights potential imidazolones derivatives rapid expansion pallet labeling tools.
Abstract Fluorescence‐activating proteins (FAPs) that bind a chromophore and activate its fluorescence have gained popularity in bioimaging. The fluorescence‐activating absorption‐shifting tag (FAST) is light‐weight FAP enables fast reversible fluorogen binding, thus advancing multiplex super‐resolution imaging. However, the rational design of FAST‐specific fluorogens with large enhancement (FE) remains challenging. Herein, new directly engineered from green fluorescent protein (GFP) by...
In this paper, we propose a fluorescence-lifetime imaging microscopy (FLIM) multiplexing system based on the fluorogen-activating protein FAST. This genetically encoded fluorescent labeling platform employs FAST mutants that activate same fluorogen but provide different fluorescence lifetimes for each specific protein-dye pair. All proposed probes with varying possess nearly identical and smallest-in-class size, along quite similar steady-state optical properties. live mammalian cells,...
The photoinduced ring-twisting motions governed by electrostatics (sterics) in the excited (ground) state are elucidated steady-state/time-resolved electronic and vibrational spectroscopies.
(2-Aminobenzylidene)-imidazolones were used as substrates for [1,5]-hydride shift triggered cyclization under promotion by TiCl<sub>4</sub> at room temperature.
Green fluorescent protein (GFP) has enabled a myriad of bioimaging advances due to its photophysical and photochemical properties. To deepen the mechanistic understanding such light-induced processes, novel derivatives GFP chromophore p-HBDI were engineered by fluorination or bromination phenolic moiety into superphotoacids, which efficiently undergo excited-state proton transfer (ESPT) in aqueous solution within short lifetime excited state, as opposed where efficient ESPT is not observed....
"Fluorescence-Activating and absorption-Shifting Tag" (FAST) is a well-studied fluorogen-activating protein with high brightness low size, able to activate wide range of fluorogens. This makes FAST promising target for both fluorogen optimization. Here, we describe the structure-based rational design enhanced mutants, optimized N871b fluorogen. Using spatial structure FAST/N871b complex, NMR relaxation analysis, computer simulations, identify mobile regions in complex suggest mutations that...
We report the creation of a novel group ABDI‐BF 2 fluorescent dyes based on conformationally locked GFP chromophore. studied intramolecular mechanism radiationless deactivation fluorophore by introducing various substituents at nitrogen atom. The results this study and our previous work allowed us to claim that in case is determined formation nonfluorescent internal charge transfer exited state with planar quinoidal structure. electronic effects have greater impact than conformation. Thus,...
A novel effective protocol towards indolo[2,1-<italic>a</italic>]isoquinolinones <italic>via</italic> aryne-induced migration of aryl-anion in aryloxy substituted 3,4-dihydroisoquinolines is reported.
We report the synthesis and characterization of a pH-sensitive fluorescence switch based on conformationally-locked green fluorescent protein (GFP) chromophore.