A5011725422- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Enzyme Structure and Function
- ATP Synthase and ATPases Research
- Computational Drug Discovery Methods
- Molecular spectroscopy and chirality
- Crystallography and molecular interactions
- Advanced Polymer Synthesis and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis C virus research
- Genetic Neurodegenerative Diseases
- Machine Learning in Materials Science
- Influenza Virus Research Studies
- Click Chemistry and Applications
- RNA Research and Splicing
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Organometallic Complex Synthesis and Catalysis
- Alzheimer's disease research and treatments
- SARS-CoV-2 and COVID-19 Research
- Free Radicals and Antioxidants
- Advanced NMR Techniques and Applications
- Cellular transport and secretion
- Advanced MRI Techniques and Applications
Max Perutz Labs
2009-2021
Vienna Biocenter
2009-2021
University of Vienna
2018-2021
Harvard University
2013-2018
Universität Innsbruck
2013
Université Joseph Fourier
2009
Université Grenoble Alpes
2009
Institut de Biologie Structurale
2009
Centre National de la Recherche Scientifique
2009
CEA Grenoble
2009
One, two, three, whee! After transfer into the gas phase, three-helix bundle protein KIX is sufficiently stabilized by electrostatic interactions to compensate for loss of hydrophobic bonding. It thus retains its global fold on a timescale more than 4 s.
Allosteric regulation is an effective mechanism of control in biological processes. In allosteric proteins a signal originating at one site the molecule communicated through protein structure to trigger specific response remote site. Using NMR relaxation dispersion techniques we directly observe dynamic process which KIX domain CREB binding communicates information between sites. mediates cooperativity pairs transcription factors two distinct interaction surfaces manner. We show that...
While CH-π interactions with target proteins are crucial determinants for the affinity of arguably every drug molecule, no method exists to directly measure strength individual in drug-protein complexes. Herein, we present a fast and reliable methodology called PI (π interactions) by NMR, which can differentiate protein-ligand solution. By combining selective amino-acid side-chain labeling 1 H-13 C able identify specific protein protons side-chains engaged aromatic ring systems ligand, based...
The KIX domain of the transcriptional coactivator CREB binding protein (CBP) co-operatively mediates interactions between transcription factors. Binding factor mixed-lineage leukemia (MLL) induces formation a low-populated conformer that resembles conformation in presence second molecule. NMR spin relaxation studies have previously shown allosteric coupling proceeds through network hydrophobic core residues bridge two sites. Here we describe high-resolution solution structures binary complex...
Eins, zwei, drei, hui! Nach dem Übergang in die Gasphase ist das Dreihelixbündelprotein KIX durch elektrostatische Wechselwirkungen ausreichend stabilisiert, um den Verlust an hydrophobem Binden zu kompensieren. Es behält deshalb seine globale Faltung auf einer Zeitskala von mehr als 4 s bei. Recently, a general picture has been proposed of how long, and to what extent, native protein structure can be retained the gas phase.1a In particular, molecular dynamics simulations suggest that salt...
Abstract While CH–π interactions with target proteins are crucial determinants for the affinity of arguably every drug molecule, no method exists to directly measure strength individual in drug–protein complexes. Herein, we present a fast and reliable methodology called PI (π interactions) by NMR, which can differentiate protein–ligand solution. By combining selective amino‐acid side‐chain labeling 1 H‐ 13 C able identify specific protein protons side‐chains engaged aromatic ring systems...
The NRF2 transcription factor is a key regulator in cellular oxidative stress response, and acts as tumor suppressor. Aberrant activation of has been implicated promoting chemo-resistance, growth, metastasis by activating its downstream target genes. Hence, inhibition promises to be an attractive therapeutic strategy suppress cell proliferation enhance apoptosis cancer. Direct targeting with small-molecules discover protein-DNA interaction inhibitors challenging it largely intrinsically...
SCaMC is an ATP-Mg/Pi carrier protein located at the mitochondrial inner membrane. has unusual N-terminal Ca(2+)-binding domain (NTD) in addition to its characteristic six-helix transmembrane bundle. The NTD of human SCaMC1 (residues 1-193) was expressed and purified order study role Ca(2+)-regulated transport mediated by domain. While Ca(2+)-bound could be crystallized, apo state resisted extensive crystallization trials. Selenomethionine-labeled crystals, which belonged space group P6(2)22...
The RAS family of GTPases is among the most frequently mutated proteins in human cancer, creating a high clinical demand for therapies that counteract their signaling activity. An important layer regulation could be therapeutically exploited proteostatic main KRAS, NRAS, and HRAS, as well closely related members, MRAS RIT1, by leucine zipper-like transcriptional regulator 1 cullin 3 RING E3 ubiquitin ligase complex (CUL3LZTR1). Genetic inactivation LZTR1, observed different cancer entities...
Die CH-π-Wechselwirkungen zwischen den CH-Donorgruppen von Trp81-ζ und Trp81-η mit aromatischen π-Systemen Ligand 3 (dargestellt in blau bzw. grün) werden im Titelbild illustriert. Der Hintergrund zeigt eine Skala der chemischen Verschiebung, welche die CH-Kreuzpeaks Trp81 eingezeichnet sind. In komplexierter Form CH-Gruppen um bis zu 2.3 ppm verschoben, da sie Ringsystemen des Liganden abgeschirmt werden. Wie G. Platzer, M. Mayer et al. ihrem Forschungsartikel auf S. 14971 erläutern, zeigen...
Informationen zur dreidimensionalen Struktur des Drei-Helix-Bündel-Proteins KIX können aus Elektroneneinfangdissoziations(ECD)-Massenspektrometriedaten gewonnen werden. In der Zuschrift auf S. 903 ff. berichten K. Breuker et al. über die Stabilisierung Proteins durch elektrostatische Wechselwirkungen nach Überführung in Gasphase.