A5087620535- Ion channel regulation and function
- Ion Channels and Receptors
- Cardiac electrophysiology and arrhythmias
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Plant Stress Responses and Tolerance
- Nicotinic Acetylcholine Receptors Study
- Marine Sponges and Natural Products
- Coral and Marine Ecosystems Studies
- Electrochemical Analysis and Applications
- Adenosine and Purinergic Signaling
- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
- Biochemical Analysis and Sensing Techniques
- DNA Repair Mechanisms
- Postharvest Quality and Shelf Life Management
- Photoreceptor and optogenetics research
- Origins and Evolution of Life
- Magnetic and Electromagnetic Effects
- Aquaculture disease management and microbiota
- Insect and Pesticide Research
- thermodynamics and calorimetric analyses
- Plant Physiology and Cultivation Studies
- Phytochemistry and biological activities of Ficus species
- Enzyme Structure and Function
Universidad Nacional Autónoma de México
2015-2024
Temperature-activated TRP channels or thermoTRPs are among the only proteins that can directly convert temperature changes into in channel open probability. In spite of a wealth functional and structural information, mechanism activation remains unknown. We have carefully characterized repeated TRPV1 by thermal stimuli discovered previously unknown inactivation process, which is irreversible. propose this form gating consequence heat absorption process leads to opening.
Voltage-dependent gating of the voltage-gated proton channels (H V 1) remains poorly understood, partly because difficulty obtaining direct measurements voltage sensor movement in form currents. To circumvent this problem, we have implemented patch-clamp fluorometry combination with incorporation fluorescent non-canonical amino acid Anap to monitor channel opening and S4 segment. Simultaneous recording currents fluorescence signals allows for correlation these parameters investigation their...
Much has been learned about the voltage sensors of ion channels since x-ray structure mammalian voltage-gated potassium channel Kv1.2 was published in 2005. High resolution structural data a Kv enabled interpretation numerous electrophysiological findings collected various channels, most notably Shaker, and permitted development meticulous computational simulations activation mechanism. The fundamental premise for functional measurements from Shaker is that this have same characteristics,...
The transient receptor potential vanilloid 1 ion channel is responsible for the perception of high temperatures and low extracellular pH, it also involved in response to some pungent compounds. Importantly, associated with pain noxious stimuli. Here, we attempt discern molecular organization location N C termini by measuring FRET between genetically attached enhanced yellow cyan fluorescent protein or terminus protein, expressed transfected HEK 293 cells Xenopus laevis oocytes. static...
Proton channels have evolved to provide a pH regulatory mechanism, affording the extrusion of protons from cytoplasm at all membrane potentials. Previous evidence has suggested that channel-mediated acid could significantly change local concentration in vicinity channel. In this work, we directly measure proton depletion caused by activation Hv1 using patch-clamp fluorometry recordings labeled with Venus fluorescent protein intracellular domains. The fluorescence is very sensitive pH, thus...
Slow inactivation has been described in multiple voltage-gated K+ channels and great detail the Drosophila Shaker channel. Structural studies have begun to facilitate a better understanding of atomic details this other gating mechanisms. To date, only potassium whose structure solved are KvAP (x-ray diffraction), KV1.2-KV2.1 “paddle” chimera diffraction cryo-EM), KV1.2 ether-à-go-go (cryo-EM); however, structural mechanisms slow these unknown or poorly characterized. Here, we present...
Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence gene encoding Hv1 voltage-dependent, in two species reef-building corals. We performed characterization their biophysical properties and found that these fast-activating modulated by pH gradient distinct manner. The novel make them interesting model systems. have also developed an allosteric gating provides mechanistic insight into modulation...
Abstract Xenopus follicle‐enclosed oocytes are endowed with purinergic receptors located in the follicular cell membrane; their stimulation by ATP elicits an electrical response that includes generation of a fast inward current ( F Cl ) carried − . Here, it was found mechanical follicle provoked native named I mec This dependent on coupling between oocyte and cells, because eliminated enzymatic defolliculation or application uncoupling drugs, such as heptanol carbenoxolone. Moreover,...
GPN-loop GTPases 1 and 3 are required for RNA polymerase II (RNAPII) nuclear import. Gpn1 Gpn3 display some sequence similarity, physically associate, their protein expression levels mutually dependent in human cells. We performed here Fluorescence Resonance Energy Transfer (FRET), molecular modeling, cell biology experiments to understand, eventually disrupt, Gpn1-Gpn3 interaction live HEK293-AD Transiently expressed EYFP-Gpn1 Gpn3-CFP generated a strong FRET signal, indicative of very...
The transient receptor potential vanilloid 4 (TRPV4) ion channel is present in different tissues including those of the airways. This activated response to stimuli such as changes temperature, hypoosmotic conditions, mechanical stress, and chemicals from plants, lipids, others. TRPV4’s overactivity and/or dysfunction has been associated with several diseases, skeletal dysplasias, neuromuscular disorders, lung pathologies asthma cardiogenic edema COVID-19–related respiratory malfunction....
The era of the molecular structure ion channels has revealed that their transmembrane segments are alpha helices, as was suspected from hydropathy analysis and experimental data. TRP recent additions to known families little structural data is available. In a work, we explored conformational changes occurring at putative S6 segment TRPV1 observed periodicity chemical modification residues suggestive an helical structure. Further disposition hydrophobic in segment, suggests general...
Abstract Temperature activated TRP channels or thermoTRPs are among the only proteins that can directly convert temperature changes into in channel open probability. In spite of a wealth information, including several experimentally determined structural models channels, mechanism activation remains unknown. We have carefully characterized repeated TRPV1 by thermal stimuli and discovered previously unknown inactivation process, which is irreversible. This associated with specific...
P2X receptors are trimeric, ATP-gated cation channels. In mammals seven subtypes have been reported (P2X1-P2X7), as well several variants generated by alternative splicing. Variants confer to the homomeric or heteromeric channels distinct functional and/or pharmacological properties. Molecular biology, biochemical, and analysis electrophysiological methods were used identify study a new variant of P2X1 receptor named P2X1b. This variant, identified in rat optic nerve, was also expressed...
Abstract The TRPV1 cation non-selective ion channel plays an essential role in thermosensation and perception of other noxious stimuli. can be activated by low pH, high temperature or naturally occurring pungent molecules such as allicin, capsaicin resiniferatoxin. Its thermal sensitivity makes it important participant a sensor mammals. However, details the mechanism activation increases remain unclear. Here we used combination approaches to try understand ankyrin repeat domain (ARD)...
Abstract C-type inactivation has been described in multiple voltage-gated K + channels and great detail the Drosophila Shaker channel. As have moved into structural era, atomic details of this other gating mechanisms started to be better understood. To date, only whose structure solved are KvAP (X-ray diffraction), V 1.2- 2.1 “paddle” chimera 1.2 (Cryo-EM); ether-á-go-go (Cryo-EM) (Wang MacKinnon, 2017), however, characteristics slow these unknown or poorly characterized. Here we present a...