A5000208646- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Animal Genetics and Reproduction
- COVID-19 Clinical Research Studies
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Cancer, Stress, Anesthesia, and Immune Response
- RNA Research and Splicing
- Renal and related cancers
- Sexual Differentiation and Disorders
- Neuropeptides and Animal Physiology
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
- Vitamin C and Antioxidants Research
- Immune responses and vaccinations
- Mesenchymal stem cell research
- SARS-CoV-2 and COVID-19 Research
- Vitamin D Research Studies
- Immune Cell Function and Interaction
Centre National de la Recherche Scientifique
2016-2025
Université de Rennes
2016-2025
Institut de génétique et de développement de Rennes
2016-2025
Wojskowy Instytut Medycyny Lotniczej
2022-2024
Narodowy Instytut Leków
2023
Wojskowy Instytut Higieny i Epidemiologii
2016-2021
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2013-2016
École Nationale Supérieure de Chimie de Rennes
2013
École Normale Supérieure - PSL
2012
Fédération de recherche en imagerie multi-modalité
2012
Oocyte meiotic maturation is triggered by different stimuli (hormones, unknown signals through cell interactions) in species. These indirectly lead to the activation of a major cycle regulating activity, promoting factor (MPF). Other factors, such as product proto-oncogene c-mos or enzymes MAP kinase family, are also involved process maturation. occurs during oocytes from species with kinetics. The relationships between MPF and have been well studied clam Xenopus. In this paper, we study...
ABSTRACT Mos is normally expressed during oocyte meiotic maturation in vertebrates. However, apart from its cytostatic factor (CSF) activity, precise role mouse meiosis still unknown. First, we analyzed as a MAP kinase kinase. synthesized concomitantly with the activation of oocytes. Moreover, not activated oocytes mos–/– mice. This result implies that necessary for Raf-1, another kinase, already present immature oocytes, but does seem to be active when activated. absence demonstrates Raf-1...
During meiosis, two successive divisions occur without any intermediate S phase to produce haploid gametes. The first meiotic division is unique in that homologous chromosomes are segregated while the cohesion between sister chromatids maintained, resulting a reductional division. Moreover, duration of M usually prolonged when compared with mitotic phases lasting 8 h mouse oocytes. We investigated spindle assembly pathway and its role progression 4 h, bipolar forms congress near equatorial...
ABSTRACT Mouse oocyte activation is followed by a peculiar period during which the interphase network of microtubules does not form and chromosomes remain condensed despite inactivation MPF. To evaluate role protein phosphorylation this period, we studied effects kinase inhibitor 6-dimethylaminopurine (6-DMAP) on fertilization and/or parthenogenetic metaphase II-arrested mouse oocytes. 6-DMAP itself induce histone H1 in oocytes, influence dynamics activation. However, inhibits after In...
ABSTRACT To study the mechanisms involved in progression of meiotic maturation mouse, we used oocytes from two strains mice, CBA/Kw and KE, which differ greatly rate at they undergo maturation. extrude first polar body about 7 hours after breakdown germinal vesicle (GVBD), whilst KE mice take approximately 3-4 longer. In both strains, kinetics spindle formation are comparable. While MAP kinase activity very similar (although slightly faster CBA/Kw), rise cdc2 is rapid slow diphasic oocytes....
ABSTRACT When metaphase Il-arrested mouse oocytes (M II) are activated very soon after ovulation, they respond abortively by second polar body extrusion followed another arrest (metaphase III, M III; Kubiak, 1989). The Il/M III transition resembles the natural between first and meiotic metaphases 1/M II). We observed that a similar sequence of events takes place during these two transitions: anaphase, is extruded, microtubules midbody disappear rapidly new spindle forms. MPM-2 monoclonal...
Translationally controlled tumor-associated protein (TCTP) is a ubiquitous and highly conserved implicated in cancers. Here, we demonstrate that interactions of TCTP with microtubules (MTs) are functionally important but indirect, reveal novel interaction the actin cytoskeleton. Firstly, immunofluorescence Xenopus XL2 cells revealed cytoplasmic fibers stained not tubulin antibodies, as well MTs free TCTP. Furthermore, localized to subset actin-rich migrating cells. Secondly, laevis did...