A5062512325- Prion Diseases and Protein Misfolding
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Plant Reproductive Biology
- Neurological diseases and metabolism
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Evolution and Genetic Dynamics
- Trace Elements in Health
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Plant nutrient uptake and metabolism
- Microbial metabolism and enzyme function
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Genetic Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Plant Gene Expression Analysis
- Acute Lymphoblastic Leukemia research
- Plant Pathogens and Resistance
- Chronic Myeloid Leukemia Treatments
- Infectious Diseases and Mycology
- Nuclear Receptors and Signaling
Robert Bosch (Germany)
2022-2024
Max Planck Institute for Biology
2022-2024
Max Planck Institute for Developmental Biology
2017-2021
University Hospital of Zurich
2008-2012
Ludwig-Maximilians-Universität München
1997-2005
University of Göttingen
1995-2002
Klinik und Poliklinik für Neurologie
2000-2002
Institute of Neuroimmunology of the Slovak Academy of Sciences
1992
Abstract Since the first half of twentieth century, evolutionary theory has been dominated by idea that mutations occur randomly with respect to their consequences 1 . Here we test this assumption large surveys de novo in plant Arabidopsis thaliana In contrast expectations, find less often functionally constrained regions genome—mutation frequency is reduced inside gene bodies and two-thirds essential genes. With independent genomic mutation datasets, including from largest accumulation...
<b><i>Background:</i></b> Diagnosis of Creutzfeldt–Jakob disease (CJD) is made according to the typical clinical picture and can be supported by a positive 14-3-3 CSF immunoblot. Promising results for diagnostic sensitivity specificity tau-protein measurement in already have been described smaller group patients. Both tests larger patients with differential diagnosis CJD were evaluated. <b><i>Methods:</i></b> 297 under (109 definite, 55 probable, 39 possible; 85 others, 1 iatrogenic, 8...
We have isolated RNA aptamers which are directed against the recombinant Syrian golden hamster prion protein rPrP23-231 (rPrPc) fused to glutathione S-transferase (GST). The did not recognize fusion partner GST or GST::rPrP90-231 (rPrP27-30), lacks 67 amino acids from PrP N terminus. aptamer-interacting region of PrPc was mapped N-terminal 23 52. Sequence analyses suggest that may fold into G-quartet-containing structural elements. Replacement G residues in quartet scaffold with uridine...
Crop disease outbreaks are often associated with clonal expansions of single pathogenic lineages. To determine whether similar boom-and-bust scenarios hold for wild pathosystems, we carried out a multi-year, multi-site survey Pseudomonas in its natural host Arabidopsis thaliana. The most common lineage corresponded to ubiquitous clade. Sequencing 1,524 genomes revealed this have diversified approximately 300,000 years ago, containing dozens genetically identifiable sublineages. There is...
The transition to flowering is a crucial step in the plant life cycle that controlled by multiple endogenous and environmental cues, including hormones, sugars, temperature, photoperiod. Permissive photoperiod induces expression of FLOWERING LOCUS T (FT) phloem companion cells leaves. FT protein then acts as florigen transported shoot apical meristem, where it physically interacts with Basic Leucine Zipper Domain transcription factor FD 14-3-3 proteins. However, despite importance promoting...
Mutations are the ultimate source of all genetic variation. However, few direct estimates contribution mutation to molecular variation available. To address this issue, we first analyzed rate and spectrum mutations in Arabidopsis thaliana reference accession after 25 generations single-seed descent. We then compared profile these accumulation (MA) lines against observed 1001 Genomes Project. The estimated haploid single nucleotide (SNM) for A. is 6.95 × 10-9 (SE ± 2.68 10-10) per site...
Programmable gene regulators that can modulate the activity of selected targets in trans are a useful tool for probing and manipulating function. CRISPR technology provides convenient method targeting also be adapted multiplexing other modifications to enable strong regulation by range different effectors. We generated vector toolbox CRISPR/dCas9-based targeted plants, modified with previously described MS2 system amplify strength regulation, using Golden Gate-based cloning rapid assembly...
Abstract Plants can produce organs throughout their entire life from pluripotent stem cells located at growing tip, the shoot apical meristem (SAM). At time of flowering, SAM Arabidopsis thaliana switches fate and starts producing flowers instead leaves. Correct timing flowering in part determines reproductive success, is therefore under environmental endogenous control. How epigenetic regulation contributes to floral transition has eluded analysis so far, mostly because poor accessibility...
Objectives: Decreased levels of Aβ1-42 are found in CSF patients with AD. Because early stages Creutzfeldt-Jakob disease (CJD) and AD share several clinical features, we investigated these groups, inferring that this might give additional help differentiating CJD from patients.
The phloem plays essential roles in the source-to-sink relationship and long-distance communication, thereby coordinates growth development throughout plant. Here we employed isolation of nuclei tagged specific cell types coupled with low-input, high-throughput sequencing approaches to analyze changes chromatin modifications H3K4me3 H3K27me3 their correlation gene expression companion cells (PCCs) Arabidopsis(Arabidopsis thaliana) shoots response photoperiod. We observed a positive between...
Twelve methods for the isolation of mycobacteria were compared by applying them in parallel to 26 samples surface water and 109 treated water. Each method was defined a particular combination decontamination method, growth medium, incubation temperature. For water, we used cetylpyridinium chloride (CPC) (30 min, 0.05%), as well sample preincubation tryptic soy broth (TSB) followed with cocktail NaOH, cycloheximide, malachite green. Treated decontaminated 0.005 0.05% CPC min). After...
Abstract The pathomechanism of sporadic Creutzfeldt–Jakob disease (sCJD) in the central nervous system is insufficiently understood. aims this study were to identify differentially regulated genes frontal cortex sCJD and profile gene expression patterns by using Affymetrix HGU133A microarrays (Affymetrix, Santa Clara, CA). microarray data generated dChip analyzed Significance Analysis Microarray (SAM) software. A comparison between control samples identified 79 upregulated 275 downregulated...
Abstract Plants are protected from pathogens not only by their own immunity but often also colonizing commensal microbes. In Arabidopsis thaliana, a group of cryptically pathogenic Pseudomonas strains dominates local populations. This coexists in nature with that can blunt the deleterious effects laboratory. We have investigated interaction between one and 99 naturally co-occurring commensals, finding plant protection to be common among non-pathogenic Pseudomonas. While protective ability is...
Summary Chronic neurodegenerative diseases are characterized by the accumulation of aggregated protein species, and functional impairment ubiquitin proteasome system has been hypothesized to contribute neuronal cell loss. Decreased proteolytic activity 20S shown postmortem in crude brain lysates from Alzheimer’s disease (AD) patients. In present study, we demonstrate, however, that catalytic increases during chromatographic purification AD brains as compared with age‐matched controls. By...
Abstract A central goal in microbiome research is to learn what distinguishes a healthy from dysbiotic microbial community. Shifts diversity and taxonomic composition are important indicators of dysbiosis, but full understanding also requires knowledge absolute population sizes. In addition the number cells, information on can provide insight into function disease state. Here we use shotgun metagenomics simultaneously assess load phyllosphere wild populations plant Arabidopsis thalian a. We...
Logopenic aphasia, mainly characterized by word anomia, sentence and phrase comprehension difficulties secondary to phonological loop deficits but relatively preserved single no agrammatism, is one of the 3 main variants primary progressive aphasia (PPA). We describe first case PPA that fulfilled clinical criteria logopenic showed abnormal DWI hyperintensities were predominant on left hemisphere compatible with Creutzfeldt-Jakob disease (CJD). After abnormally long isolated language...
Summary Microbes affect plant health, stress tolerance 1 and life history 2 . In different regions of the globe, plants are colonized by distinct pathogenic commensal microbiomes, but factors driving their geographic variation largely unknown 3 We identified measured core leaf microbiome Arabidopsis thaliana in its native range, from almost 300 populations across Europe. Comparing distribution approximately 500 major bacterial phylotypes, we discovered marked, geography-dependent differences...
Transmissible mink encephalopathy (TME) is a rare disease of the North American mink, which has never been successfully transmitted to laboratory mice. We generated transgenic mice expressing prion protein (PrP) and inoculated them with TME or mouse-adapted scrapie strain 79A. infected PrP-transgenic on murine PrP knockout background. The absolute species barrier between infectious agent was therefore broken. Following 79A infection carrying both PrP(C), only proteinase-resistant homologous...
Abstract Plants are colonized by distinct pathogenic and commensal microbiomes across different regions of the globe, but factors driving their geographic variation largely unknown. Here, using 16S ribosomal DNA shotgun sequencing, we characterized associations Arabidopsis thaliana leaf microbiome with host genetics climate variables from 267 populations in species’ native range Europe. Comparing distribution 575 major bacterial amplicon variants (phylotypes), discovered that composition A....