A5021360333- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Microbial Metabolism and Applications
- Microbial infections and disease research
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Tuberculosis Research and Epidemiology
- Microbial Natural Products and Biosynthesis
- Microbial Metabolites in Food Biotechnology
- NF-κB Signaling Pathways
- Protein Structure and Dynamics
- Phytochemical Studies and Bioactivities
- Monoclonal and Polyclonal Antibodies Research
- Plant-Microbe Interactions and Immunity
- Heme Oxygenase-1 and Carbon Monoxide
- Mycobacterium research and diagnosis
- Bacillus and Francisella bacterial research
- Cancer therapeutics and mechanisms
- Potato Plant Research
- Quinazolinone synthesis and applications
- Inflammasome and immune disorders
University of Tübingen
2015-2023
Birla Institute of Technology and Science, Pilani
2020
Birla Institute of Technology and Science - Hyderabad Campus
2020
Czech Academy of Sciences, Institute of Microbiology
2019
Urology Team
2013
Persister cells constitute a small subpopulation of bacteria that display remarkably high antibiotic tolerance and for pathogens such as Staphylococcus aureus are suspected culprits chronic recurrent infections. Persisters formed during exponential growth characterized by low ATP levels but less is known in stationary phase. By enrichment from transposon mutant library S. we identified mutants this phase displayed enhanced persister cell formation. We found inactivation either sucA or sucB,...
Abstract Background Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. However, the rapid emergence antibiotic resistance limits choice therapeutic options for treating infections caused by this organism. Muralytic enzymes from bacteriophages have recently gained attention their potential as antibacterial agents against antibiotic-resistant gram-positive organisms. Phage K polyvalent virulent phage Myoviridae family that active many species. Results We...
Significance The QS Agr system of the pathogen S. aureus is a social trait based on bacterial population density that orchestrates expression toxins crucial for virulence. Paradoxically, Agr-defective mutants are selected during infection. We studied selection and fitness agr under infection-relevant conditions, such as antibiotic stress hypoxia. Under aerobic growth, Agr-controlled PSM produced. These toxic Staphylococci select mutants, which nonproducers. Contrastingly, hypoxia favors...
Numerous organisms, including bacteria, are endowed with a 5′ NAD cap in specific RNAs. While the presence of modulates stability modified RNA species, significant biological function and phenotype have not been assigned so far. Here, we show RNAIII from S. aureus , dual-function regulatory involved quorum-sensing processes regulation virulence factor expression. We also demonstrate that altering natural modification ratio leads to decrease exotoxin production, thereby modulating bacterium’s...
Bacteria respond to ever-changing environments through several adaptive strategies. This includes mechanisms leading a high degree of phenotypic variability within genetically homogeneous population. In Staphylococcus aureus, the capsular polysaccharide (CP) protects against phagocytosis, but also impedes adherence endothelial cells and/or matrix proteins. We analysed regulation core biosynthesis genes (capA-P) necessary for CP synthesis using single-cell assays (immunofluorescence and...
Capsular polysaccharide (CP) biosynthesis in Staphylococcus aureus is tightly controlled resulting a heterogeneous phenotype within population and CP being mainly detectable nongrowing cells. Expression of the corresponding gene cluster driven by one promoter element (Pcap ). Here, we demonstrate that Pcap contains main SigB-dependent promoter. The SigB consensus motif overlaps with previously described inverted repeat (IR) crucial for cap expression. essentiality IR derived from this region...
Abstract Background Antibiotic resistant S. aureus infection is a global threat. Newer approaches are required to control this organism in the current scenario. Cell wall degrading enzymes have been proposed as antibacterial agents for human therapy. P128 novel antistaphylococcal chimeric protein under development against use which derives its bacterial cell catalytic endopeptidase domain from ORF56, Phage K tail-structure associated enzyme. Lead therapeutic entities be extensively...
P128 is a chimeric anti-staphylococcal protein having catalytic domain from Staphylococcus bacteriophage K tail associated structural and cell wall targeting the bacteriocin-lysostaphin. In this study, we disclose additional properties of compared same with lysostaphin. While lysostaphin was found to get inactivated by heat inactive on its parent strain S. simulans biovar staphylolyticus, thermostable lytic towards staphylolyticus demonstrating difference in their mechanism action. Selected...
asymptomatically colonizes the nasal cavity of mammals, but it is also a leading cause life-threatening infections. Most human isolates carry Sa3 phages, which integrate into bacterial
Bacteriophages represent a rich and unique resource of anti-infectives to counter the global problem antibiotic resis- tance. In this work, we assessed bactericidal activity two virulent staphylococcal phages, K 44AHJD, against S. aureus isolates clinical significance, tested their efficacy in vivo. The phage cocktail lysed >85% tested. Both phages were purified by ion-exchange column chromatography following propagation bioreactors. purity profiles compared with those using cesium chloride...
Staphylococcus aureus are gram-positive bacteria responsible for a wide array of diseases, ranging from skin and soft tissue infections to more chronic illnesses such as toxic shock syndrome, osteomyelitis, endocarditis. Vancomycin is currently one the most effective antibiotics available in treating patients infected with methicillin-resistant S. (MRSA), however emergence vancomycin-resistant (VRSA), commonly vancomycin-intermediate (VISA), threaten future efficacy vancomycin. Intermediate...
During antibiotic persistence, bacterial cells become transiently tolerant to antibiotics by restraining their growth and metabolic activity. Detailed molecular characterization of persistence is hindered low count persisting the need for isolation. Here, we used sustained addition stable isotope-labeled lysine selectively label proteome during hipA-induced hipB-induced resuscitation Escherichia coli in minimal medium after treatment. Time-resolved, 24-h measurement incorporation allowed...
The aim of our research was the isolation steroidal alkaloid glycosides such as anguivine and isoanguivine from dried roots S. anguvi evaluation their anti-inflammatory activity by HRBC carrageenan induced paw edema method. PASS programme predicts aglycon part spirosolenol, on basis a similarity structure based drug design. Molecular docking studies with human phospholipase A2 enzyme were carried outby utilizing Argus lab 4.0.1.version. in silico study pharmacological correlated good agreement.
1 Abstract Nicotinamide adenosine dinucleotide (NAD) has been found to be covalently attached the 5’-ends of specific RNAs in many different organisms, but physiological consequences this modification are largely unknown. Here we report occurrence several NAD-RNAs opportunistic human pathogen Staphylococcus aureus . Most prominently, RNAIII, a central quorum-sensing regulator bacterium’s physiology, was 5’-NAD-capped significant extent. NAD incorporation efficiency into RNAIII depend vivo on...
Abstract In Staphylococcus aureus , the capsular polysaccharide (CP) protects against phagocytosis, but also hinders adherence to endothelial cells and matrix proteins. Its biosynthesis is tightly controlled resulting in a heterogeneous phenotype within population CP being mainly detectable non-growing cells. Capsular genes are encoded by conserved capA-P operon whose expression driven an upstream promoter element (P cap ) front of capA . The organization P poorly understood, as interplay...
Staphylococcus aureus are gram positive bacteria responsible for a wide array of diseases, ranging from skin and soft tissue infections to more chronic illnesses such as toxic shock syndrome, osteomyelitis, endocarditis. Vancomycin is currently one the most effective antibiotics available in treating patients infected with methicillin-resistant S. (MRSA), however emergence vancomycin-resistant (VRSA), commonly vancomycin-intermediate (VISA), threatens future efficacy vancomycin. Intermediate...
Abstract During antibiotic persistence, bacterial cells become transiently tolerant to antibiotics by restraining their growth and metabolic activity. Detailed molecular characterization of persistence is hindered low count persisting the need for isolation. Here we used sustained addition stable isotope-labeled lysine selectively label proteome during hipA -induced hipB resuscitation E. coli in minimal medium after treatment. Time-resolved, 24-hour measurement incorporation allowed...