A5091655527- Cancer Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Cancer Immunotherapy and Biomarkers
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- Prostate Cancer Treatment and Research
- SARS-CoV-2 detection and testing
- Mast cells and histamine
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Bladder and Urothelial Cancer Treatments
- Drug Transport and Resistance Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Cancer Mechanisms and Therapy
- Cancer, Stress, Anesthesia, and Immune Response
- Inflammatory Biomarkers in Disease Prognosis
- Cervical Cancer and HPV Research
- Animal Virus Infections Studies
- Immune cells in cancer
- Viral Infections and Outbreaks Research
- Monoclonal and Polyclonal Antibodies Research
- Ginger and Zingiberaceae research
- Cancer Cells and Metastasis
- Chemokine receptors and signaling
Frederick National Laboratory for Cancer Research
2023-2025
National Cancer Institute
2010-2020
Center for Cancer Research
2020
Johns Hopkins Medicine
2013-2016
Johns Hopkins University
2013-2016
University of Baltimore
2015
National Institutes of Health
2010-2013
Despite the success of immune checkpoint blockade therapy in treatment certain cancer types, only a small percentage patients with solid malignancies achieve durable response. Consequently, there is need to develop novel approaches that could overcome mechanisms tumor resistance inhibition. Emerging evidence has implicated phenomenon plasticity or acquisition mesenchymal features by epithelial cells, as an mechanism.
BACKGROUND Mast cells are of interest in prostate cancer because they possess both pro- and anti-tumorigenic properties present the tumor microenvironment. We studied association mast cell count densities with recurrence using tissue microarrays (TMAs) for 462 men who recurred (cases) controls that were matched to cases nested a cohort radical prostatectomy patients. METHODS Dual-immunostaining tryptase epithelial cytokeratin-8 whole slide image analysis used assess total number, density...
The Coronavirus disease 2019 (COVID-19) pandemic presented the scientific community with an immediate need for accurate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology assays, resulting in expansion of assay development, some without following a rigorous quality control and validation, wide range performance characteristics. Vast amounts data have been gathered on SARS-CoV-2 antibody response; however, ability to compare results challenging. This study seeks analyze...
Introduction The first vaccines approved against SARS-CoV-2, mRNA-1273 and BNT162b2, utilized mRNA platforms. However, little is known about the proteomic markers pathways associated with host immune responses to vaccination. In this proof-of-concept study, sera from male female vaccine recipients were evaluated for immunologic 1-month 6-months following homologous third Methods An aptamer-based (7,289 marker) assay coupled traditional serology was leveraged generate a comprehensive...
Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency complimentary oligonucleotides stability of the duplexes with RNA or DNA. We synthesized 15/16-mer PNA probes detect HER2 mRNA. The performance these target was evaluated by fluorescence imaging bead assays. sufficiently discriminated between wild type mutant single base mismatches. Furthermore, exhibited excellent linear...
Dietary carcinogens, such as 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), and chronic inflammation have each been implicated etiologic agents in prostate cancer. We hypothesized that bacterial prostatitis would accelerate PhIP-induced preinvasive lesions the rat prostate. Male Fischer 344 rats were assigned into 4 groups: Control (untreated), PhIP (200 ppm diet for 20 weeks), Escherichia coli (E. coli, prostatic inoculation week 10), or + E. coli. Study animals monitored a total...
Infection of xenotransplanted human cells by xenotropic retroviruses is a known phenomenon in the scientific literature, with examples cited since early 1970s. However, arguably, until recently, importance this had not been largely recognized. The emergence and subsequent debunking Xenotropic Murine leukemia virus-Related Virus (XMRV) as cell culture contaminant opposed to potential pathogen several diseases, notably prostate cancer Chronic Fatigue Syndrome, highlighted problem murine...
SARS-CoV-2 persists in certain populations, even with vaccination and boosters. Emerging evidence suggests that reductions virus transmission infection will likely require involvement of the mucosal immune system, especially secretory antibodies upper respiratory tract. The Clinical Translational Serology Task Force (CTTF) within National Cancer Institute (NCI)'s Serological Sciences Network for COVID-19 (SeroNet) hosted a workshop to review status development standardization sample...
The COVID-19 pandemic highlighted the need for infrastructure to respond public health emergencies and regulate associated commercial assay development. During emergency, biomedical laboratories test developers rapidly developed serology assays SARS-CoV-2 antibodies, with varied often inadequate performance.1Pinto LA Shawar RM O'Leary B et al.A trans-governmental collaboration independently evaluate assays.Microbiol Spectr. 2022; 10e0156421Crossref Scopus (7) Google Scholar, 2Shuren J...
Enzyme-linked immunosorbent assays (ELISAs) have been used to measure anti-human-papillomavirus (HPV) immunoglobulin IgG. The goal of this study was evaluate the reproducibility ELISAs measuring different HPV isotypes, IgG1, 2, 3, and 4, IgA, IgM, against HPV16.
Abstract Prostate cancer afflicts African American men to a greater degree than European men, with 60% incidence and 2-3 times mortality. In addition, studies have shown that the rate of distant metastasis is 4 in consistent notion prostate more aggressive Americans. Of interest, are also significantly likely present inflammation benign biopsies, higher expression number inflammatory markers tissue from Americans compared One immune cell type particular interest context mast cells, which...
Abstract Evidence suggests that there is a strong association between chronic inflammation and prostate cancer development and/or progression. While known to serve as an “enabling characteristic” in many cancers, the mechanism whereby it contributes carcinogenesis progression still very controversial. This particularly true for cells of innate immune system, such mast cells, have been shown possess both pro- anti-tumor properties. The role particular interest, evidence they influence...
Abstract The goal of the current study is to establish mechanism by which mast cells may serve as a prognostic factor in and possibly explain racial disparity associated with prostate cancer. There increasing evidence for strong association between chronic inflammation cancer, role cancer are particular interest they have been suggested influence progression immune modulation. It well established that presents significant disparities, African American (AA) men being 65% more likely be...
<p>Supplementary Figure S5. Mast cell quantification in ventral prostates of control versus PhIP-treated rats (n=2) at week 20 the study (after weeks receiving dietary PhIP).</p>
<p>Supplementary Figure S2. A single <i>E. coli</i> infection of the rat prostate induces long-term chronic inflammation that persists up to 42 weeks.</p>
<p>Supplementary Figure S3. Body weight and food intake data.</p>
<p>Supplementary Methods file</p>
<p>Supplementary Figure S4. IHC for <i>E. coli</i> in rat prostate tissues at 42 weeks post-inoculation.</p>
<p>Supplementary Methods file</p>
<p>Supplementary Figure S3. Body weight and food intake data.</p>
<div>Abstract<p>Dietary carcinogens, such as 2-amino-1-methyl-6-phenylimidazo[4,5-<i>b</i>]pyridine (PhIP), and chronic inflammation have each been implicated etiologic agents in prostate cancer. We hypothesized that bacterial prostatitis would accelerate PhIP-induced preinvasive lesions the rat prostate. Male Fischer 344 rats were assigned into 4 groups: Control (untreated), PhIP (200 ppm diet for 20 weeks), <i>Escherichia coli</i> (<i>E....