A5103176678- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Advanced Biosensing Techniques and Applications
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Chemical Synthesis and Analysis
- Osteoarthritis Treatment and Mechanisms
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Microfluidic and Capillary Electrophoresis Applications
- Cellular Mechanics and Interactions
- Nanofabrication and Lithography Techniques
Singapore-MIT Alliance for Research and Technology
2019-2024
National University of Singapore
2014-2018
Mesenchymal stromal cells (MSCs) are promising therapeutic agents for cartilage regeneration, including the potential of to promote chondrogenesis in vivo. However, process development and regulatory approval MSCs as cell therapy products benefit from facile vitro approaches that can predict potency a given production run. Current standard include 21 day 3D differentiation assay followed by quantification matrix proteins. We propose novel biophysical marker is population-based be measured...
Secreted active proteases, from families of enzymes such as matrix metalloproteinases (MMPs) and ADAMs (a disintegrin metalloproteinases), participate in diverse pathological processes. To simultaneously measure multiple specific protease activities, a series parallel enzyme reactions combined with inhibitor analyses for proteolytic activity analysis (PrAMA) are essential but limited due to the sample quantity requirements complexity performing reactions. address these issues, we developed...
Abstract Microcarriers are synthetic particles used in bioreactor‐based cell manufacturing of anchorage‐dependent cells to promote proliferation at efficient physical volumes, mainly by increasing the surface area‐to‐volume ratio. Mesenchymal stromal (MSCs) adherent that for numerous clinical trials autologous and allogeneic therapy, thus requiring avenues large‐scale production efficiently low volumes cost. Here, a dissolvable gelatin‐based microcarrier is developed MSC expansion. This...
In the context of tumor analysis, implementation precision medicine requires on-time clinical measurements, which rapid large-scale single-cell screening that obtains cell population distributions and functions in tumors to determine disease progression for therapeutics. this study, a high-throughput (HTS) platform integrating optical fluorescence detectors computational method was developed as droplet-based microfluidic flow cytometer (Droplet-μFC) comprehensively analyze multiple...
Abstract A critical initial stage of skeletal morphogenesis involves formation highly compact aggregates mesenchymal cells, known as condensations, appearing regularly-spaced pattern spots. Conventional computational models to understand their patterning have been based on chemotaxis, haptotaxis, and reaction-diffusion equations. In this work, we investigate the condensations from a different perspective, namely topological defects within liquid crystal-like pattern. Using bone...