A5037031342- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Near-Field Optical Microscopy
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Hydrogels: synthesis, properties, applications
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- Luminescence and Fluorescent Materials
- Optical Imaging and Spectroscopy Techniques
- Photochromic and Fluorescence Chemistry
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Photoreceptor and optogenetics research
- Supramolecular Self-Assembly in Materials
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- TiO2 Photocatalysis and Solar Cells
- Digital Holography and Microscopy
- Micro and Nano Robotics
- Optical Coherence Tomography Applications
- Force Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- Advanced Materials and Mechanics
- Machine Learning in Materials Science
University of Göttingen
2020-2025
Czech Academy of Sciences, Institute of Physics
2024
RWTH Aachen University
2016-2018
Abstract This study introduces a novel solution to the design of structured catalysts, integrating single‐piece 3D printing with single‐atom catalysis. Structured catalysts are widely employed in industrial processes, as they provide optimal mass and heat transfer, leading more efficient use catalytic materials. They conventionally prepared using ceramic or metallic bodies, which then washcoated impregnated catalytically active layers. However, this approach may lead adhesion issues latter....
Abstract The in situ imaging of soft matter is paramount importance for a detailed understanding functionality on the nanoscopic scale. Although super‐resolution fluorescence microscopy methods with their unprecedented capabilities have revolutionized research life sciences, this potential has been far less exploited materials science. One main obstacles more universal application super‐resolved limitation readily available suitable dyes to overcome diffraction limit. Here, we report novel...
Fluorescence lifetime imaging microscopy is an important technique that adds another dimension to intensity and color acquired by conventional microscopy. In particular, it allows for multiplexing fluorescent labels have otherwise similar spectral properties. Currently, the only super-resolution capable of recording super-resolved images with information stimulated emission depletion contrast, all single-molecule localization (SMLM) techniques employ wide-field cameras completely lack...
DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable multi-target (multiplexing) bio-imaging. However, multiplexed of cells still challenging due to the dense and sticky environment inside cell. Here, we combine fluorescence lifetime microscopy (FLIM) with DNA-PAINT use information as multiplexing parameter targets identification. In contrast Exchange-PAINT, PAINT (FL-PAINT) can image multiple simultaneously does not...
A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-lifetime single-molecule localization (FL-SMLM). The synergy SMLM and imaging (FLIM) combines superior image resolution with lifetime information can be realized using two complementary experimental approaches: confocal-laser scanning (CLSM) or wide-field microscopy. Here, we systematically comprehensively compare these novel FL-SMLM approaches in different spectral regions. For FL-SMLM, use a commercial...
The in situ nanoscopic imaging of soft matter polymer structures is importance to gain knowledge the relationship between structure, properties, and functionality on scale. Cross-linking chains effects viscoelastic properties gels. correlation mechanical with distribution amount cross-linkers relevant for applications a detailed understanding polymers molecular We introduce super-resolution fluorescence-microscopy-based method visualizing quantifying cross-linker points systems. A novel...
Abstract Super‐resolution fluorescence microscopy allows for unprecedented in situ visualization of biological structures, but its application to materials science has so far been comparatively limited. One the main reasons is lack powerful dyes that allow labeling and photoswitching systems. In this study it shown appropriate substitution diarylethenes bearing a fluorescent closed dark open form paves way imaging nanostructured with three most popular super‐resolution methods are based on...
Fluorescence lifetime imaging (FLIM) has become an important microscopy technique in bioimaging. The two most of its applications are lifetime-multiplexing for many different structures parallel, and lifetime-based measurements Förster resonance energy transfer. There principal FLIM techniques, one based on confocal-laser scanning (CLSM) time-correlated single-photon counting (TCSPC) the other wide-field phase fluorometry. Although first approach (CLSM-TCSPC) assures high sensitivity allows...
Water molecules play an important role in the structure, function, and dynamics of (bio-) materials. A direct access to number water nanoscopic volumes can thus give new molecular insights into materials allow for fine-tuning their properties sophisticated applications. The determination local content has become possible by finding that H
Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists remarkable gap between lateral axial which is by factor 2 to 3 worse. One recently developed method close this metal-induced energy transfer (MIET) imaging, achieves an resolution down nanometers. It exploits distance-dependent quenching fluorescence when fluorescent molecule brought metal surface. In present manuscript, we combine...
Single-molecule localization microscopy (SMLM) at cryogenic temperature opens new avenues to investigate intact biological samples the nanoscale and perform cryo-correlative studies. Genetically encoded fluorescent proteins (FPs) are markers of choice for cryo-SMLM, but their reduced conformational flexibility below glass-transition hampers efficient cryo-photoswitching. We investigated cryo-switching rsEGFP2, one most reversibly switchable ambient due facile cis–trans isomerization...
Abstract In the burgeoning field of super‐resolution fluorescence microscopy, significant efforts are being dedicated to expanding its applications into 3D domain. Various methodologies have been developed that enable isotropic resolution at nanometer scale, facilitating visualization subcellular structures with unprecedented clarity. Central this progress is need for reliable biologically compatible validating capabilities. Choosing optimal standard poses a considerable challenge,...
Abstract Near‐equilibrium stimulus‐responsive polymers have been used extensively to introduce morphological variations in dependence of adaptable conditions. Far‐less‐well studied are triggered transformations at constant These require the involvement metastable states, which either able approach equilibrium state after deviation from metastability or can be frozen on returning nonequilibrium equilibrium. Such functional macromolecular systems hold great promise for on‐demand...
Abstract Die In‐situ‐Bildgebung weicher Materie ist grundlegend für ein tieferes Verständnis von Funktionalität auf nanoskopischer Ebene. Während superhochauflösende Fluoreszenzmikroskopiemethoden mit ihren bis dahin unerreichten Möglichkeiten der Bildgebung die Lebenswissenschaften revolutionierten, wurde dieses Potenzial in den Materialwissenschaften bisher kaum ausgeschöpft. Dabei stellt begrenzte Verfügbarkeit an geeigneten Farbstoffen zur Überwindung des Beugungslimits eines...
Single-Molecule Localization Microscopy (SMLM) has become one of the most important methods super-resolution fluorescence microscopy. It is based on precise localization single molecules in wide-field microscopy images. well known that accuracy can show a significant bias if imaged have fixed orientation and are located either close to an interface or not exactly within focal plane microscope. In this Letter, we propose simple solution problem, which polarization-resolved imaging. This...
In recent years, Image Scanning Microscopy (ISM) has emerged as a powerful technique for achieving super-resolution bio-imaging across various applications. Particularly noteworthy is the implementation of single-photon detector array, enabling utilization Lifetime Microscopy, which proven to be highly effective. our study, we present novel approach that combines ISM with direct Stochastic Optical Reconstruction (dSTORM), resulting in doubling localization precision Single Molecule...
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this information can be used, for example, quantify energy transfer efficiency in Förster Resonance Energy Transfer (FRET) imaging, probe local environment with dyes that change their an environment-sensitive manner, or achieve image multiplexing using different lifetimes. We present a thorough theoretical...