A5070014926- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Hepatitis Viruses Studies and Epidemiology
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Respiratory viral infections research
- interferon and immune responses
- Heat shock proteins research
- Studies on Chitinases and Chitosanases
- Escherichia coli research studies
- Plant Virus Research Studies
- HIV Research and Treatment
- Genomics and Phylogenetic Studies
- Pregnancy and preeclampsia studies
- Cardiomyopathy and Myosin Studies
- Nuclear Structure and Function
- Plant and Fungal Interactions Research
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Acute Kidney Injury Research
- Pharmacological Effects of Natural Compounds
Instituto Politécnico Nacional
2012-2024
Center for Research and Advanced Studies of the National Polytechnic Institute
2014-2024
Universidad Autónoma de la Ciudad de México
2015
Background: Contrast-induced nephropathy (CIN) is a complex syndrome of acute that occurs following infusion intravascular contrast agents, and associated with an increased risk for adverse cardiovascular events. While there no ideal biomarker making early diagnosis CIN, we hypothesized levels specific circulating microRNA (miRNA) species might serve such role. Methods: miRNA microarray assays were used to detect miRNAs in the kidney tissue rats studied as animal model CIN. Real-time PCR was...
During flavivirus infection, some viral proteins move to the nucleus and cellular components are relocated from cytoplasm. Thus, integrity of main regulator nuclear-cytoplasmic transport, nuclear pore complex (NPC), was evaluated during infection with dengue virus (DENV) Zika (ZIKV). We found that while DENV distribution at least three nucleoporins (Nup), Nup153, Nup98, Nup62 were altered, ZIKV TPR, Nup98 modified. In this work, several lines evidence indicate serine protease NS2B3 is...
The Cuatro Ciénegas Basin (CCB) is located in the Chihuahuan desert Mexican state of Coahuila; it has been characterized as a site with high biological diversity despite its extreme oligotrophic conditions. It greatest number endemic species North America, containing abundant living microbialites (including stromatolites and microbial mats) diverse communities. With hypothesis that this biodiversity geographic structure should be reflected virome, viral communities 11 different locations...
ABSTRACT Cellular proteins play many important roles during the life cycle of all viruses. Specifically, host cell nucleic acid-binding interact with viral components positive-stranded RNA viruses and regulate translation, as well replication. Here, we report that nucleolin, a ubiquitous multifunctional nucleolar shuttling phosphoprotein, interacts Norwalk virus feline calicivirus (FCV) genomic 3′ untranslated regions (UTRs). Nucleolin can also form complex in vitro recombinant NS6 -7...
Sequences and structures within the terminal genomic regions of plus-strand RNA viruses are targets for binding host proteins that modulate functions such as translation, replication, encapsidation. Using murine norovirus 1 (MNV-1), we describe presence long-range RNA-RNA interactions were stabilized by cellular proteins. The potentially responsible stabilization selected based on their ability to bind MNV-1 genome and/or having been reported be involved in interactions. Cell extracts...
ABSTRACT The lack of a susceptible cell line and an animal model for Norwalk virus (NV) infection has prompted the development alternative strategies to generate in vitro RNAs that approximate authentic viral genome. This approach allowed study RNA replication gene expression. In this study, using mobility shift cross-linking assays, we detected several cellular proteins from HeLa CaCo-2 extracts bind to, form stable complexes with, first 110 nucleotides 5′ end NV genomic RNA, region...
Abstract Few studies exist regarding the frequency of human caliciviruses as single etiologic agents in sporadic cases, or outbreaks occurring children hospitalized for acute gastroenteritis. In this study, a total 1,129 <5 years age and due to diarrhea were enrolled from three main hospitals Mexico City during period 3 (March 1998 December 2000). After analyzing all fecal samples several enteropathogens, 396 stools that remained negative further screened by RT‐PCR using primer set...
The leader of the capsid (LC) protein is exclusive to Vesivirus genus, and it needed for successful feline calicivirus (FCV) replication, as well an efficient apoptosis induction through mitochondrial pathway. In this work, we aimed determine if LC from FCV a viroporin. Although lacking in transmembrane domain or amphipathic helix, toxic when expressed bacteria oligomerizes disulfide bonds, which are both key characteristics viroporins. An electron microscopy analysis LC-expressing E. coli...
The stress of the Golgi apparatus is an autoregulatory mechanism that induced to compensate for greater demand in functions. No examples responses due physiological stimuli are known. Furthermore, impact on this organelle viral infections occupy vesicular transport during replication unknown. In work, we evaluated if a response triggered dengue and Zika viruses replication, two flaviviruses whose replicative cycle heavily involved with complex, vertebrate mosquito cells. Using GM-130 as...
Flaviviruses have a cytoplasmic replicative cycle, and crucial events, such as genome translation replication, occur in the endoplasmic reticulum. However, some viral proteins, C, NS1, NS5 from Zika virus (ZIKV) containing nuclear localization signals (NLSs) export (NESs), are also located nucleus of Vero cells. The NS2A, NS3, NS4A proteins dengue (DENV) been reported to be A549 cells, our group recently that NS3 protein is Huh7 C636 cells during DENV infection. protease-helicase ZIKV...
ABSTRACT Feline calicivirus (FCV), a member of the Vesivirus genus and model for studying members Caliciviridae , has been used to understand biology viral replication etiological agents medical veterinary importance. The leader capsid (LC) protein is exclusive whose importance successful demonstrated; however, its cell localization, post-translational modifications, specific functions throughout cycle are poorly understood. We have determined that expression LC from FCV in virus-free system...
(FCV), an important model for studying the biology of