Chronic radicular pain can occur after disc pathology and failed back surgery. An evidence-based effective therapeutic option is not available nor does a gold standard exist.A randomized controlled trial to analyze the clinical efficacy of percutaneous epidural lysis adhesions in chronic pain.Prospective placebo interventional trial. Power calculation based on feasibility trial.Medical university centers.Within 4 years total 381 patients with lasting longer than months which respond...

The aim of this study was to establish a simple and safe method anaesthesia for intravital microcirculatory observations in small laboratory animals. usefulness isoflurane inhalation has been investigated different strains mice commonly used experimental medicine. These were the hairless (hr/hr, n = 12), BALB/c ( 12) nude mouse (nu/nu, 3). Anaesthesia maintained by mask vaporized at concentrations up 4% induction phase, 1.5% during acute surgical procedures 0.8-1.3% prolonged observations....

Sequestration of neutrophils and release histotoxic mediators are considered important for the development pathologic alterations lung defined as adult respiratory distress syndrome. Mechanisms inflammatory injury caused by abdominal sepsis were investigated using colon ascendens stent peritonitis (CASP) model that closely mimics human disease. In CASP model, a continuous leakage intraluminal bacteria into peritoneal cavity is induced implantation in ascending colon, generating septic focus....

At many institutions, it is not practically feasible to perform a series of controlled or placebo-controlled medial branch blocks on several facet joints in order select patients for joint rhizotomy. As uncontrolled blocks, there no proof that are superior other types blocks. This study was performed compare simple pericapsular the selection lumbar cryodenervation.Patient based history, imaging, and physical examination. Diagnostic were either Percutaneous cryodenervation by use Lloyd...

There is growing evidence that platelets play an important role in the development and maintenance of rheumatoid arthritis. Activation adherence synovial microcirculation might be part responsible for endothelial damage activation leukocytes. Recent findings show a direct influence P-selectin on platelet- leukocyte-endothelial cell interaction mice with Antigen-induced Arthritis (AiA). only expressed by cells, not Therefore, aim present study was to investigate differential platelet extent...

Objective Inhibition of nitric oxide (NO) produced by inducible NO synthase (iNOS) is suggested to be beneficial in experimental arthritis. Although important for the integrity microcirculation, effects inhibition iNOS on synovial microcirculation are not currently known. This study investigated and leukocyte–endothelial cell interactions iNOS-deficient mice with antigen-induced arthritis (AIA) compared these findings disease severity. Methods Fourteen homozygous iNOS−/− 14 iNOS+/+ were...

Growing evidence supports the substantial pathophysiological impact of platelets on development rheumatoid arthritis. At present there are no methods for studying these cellular mechanisms in vivo. The aim this study was to visualize and investigate platelet-endothelial cell interaction knee joint mice with antigen-induced arthritis (AiA) by means intravital microscopy.In 14 (Balbc) microscopic assessment performed day 8 after AiA induction two groups (controls, AiA). severity assessed...

Objective: Platelets are thought to participate in the pathogenesis of chronic inflammatory diseases such as rheumatoid arthritis (RA). We showed recently an vivo increase platelet–endothelial cell interactions mice with antigen‐induced (AiA). The underlying mechanisms not yet clear. aim this study was investigate impact P‐selectin AiA by means intravital fluorescence microscopy (IVM).Methods: C57/Bl6 and P‐selectin‐deficient were divided into four groups (n = 7; control/AiA per strain)....

The microcirculation and leukocyte-endothelial cell interaction in synovial tissue of an inflamed joint are known to play a crucial role the pathogenesis rheumatoid arthritis. aim this study was characterize vivo changes microvasculature interactions mouse using intravital fluorescence microscopy three stages antigen-induced expression E- P-selectin ICAM-1 were also studied immunohistochemistry.Antigen-induced arthritis (AiA) produced Balb/c mice. severity at different phases quantified...

Background Insufficient detoxification caused by nitric oxide-related inhibition of cytochrome P450 may be important for metabolism numerous drugs, including vecuronium. The present study investigated the pharmacodynamics and pharmacokinetics vecuronium in rats with inflammatory liver dysfunction. Methods Male Sprague-Dawley (n = 56) were randomly allocated into two groups: In sepsis group, inflammation was established injection 56 mg/kg heat-killed Corynebacterium parvum; control received...

We evaluated the clinical outcome of tripled semitendinosus tendon ACL reconstruction with femoral Endobutton (Acufex, Smith&Nephew, Andover, MA) and tibial Suture Washer (n 29) or post screw fixation 6) in 35 patients on an average 28 (20-37) months after surgery. On basis IKDC criteria, 22 showed a normal nearly knee function 25 had KT 1000 maximal manual side-to-side difference < =5 mm at follow-up. Subjectively, graded their as normal. The Lysholm score was 88 points, OAK 90 points...

The aim of this study was to investigate whether all sizes wear particles are capable provoking inflammatory responses and there different among particle sizes. knees 40 female Balb/c mice were injected with polystyrene three diameters, 0.5 μm, 2.0 75 using a 0.1% vol/vol concentration. Seven days after injection, assessment the synovial microcirculation intravital microscopy, histologic examination, done. All had enhanced leukocyte-endothelial cell interactions scores regardless size when...

Abstract The purpose of the present study was to examine changes in synovial microcirculation as well tissue responses exposure titanium, polymethylmethacrylate (PMMA), ceramic (Al 2 O 3 ), cobalt–chromium alloy (Co–Cr), and polyethylene (PE) particles an vivo model. particulate biomaterials were injected into left knee joint female Balb/c mice assessment using intravital fluorescence microscopy histological evaluation response performed on day 7 after particle administration. Intravital...

Background The aim of this study was to investigate changes in the blood microcirculation skin, subcutaneous tissue, and striated muscle, venous lymphatic outflow from hind limb, after a standardized mechanical trauma. Methods Trauma, defined as 50% minimal energy needed for tibia fracture (3.7 J/g), applied leg hairless mice. Intravenously injected fluorescein isothiocyanate–dextran 150 kDa Rhodamine-6G were used intra-vital fluorescence microscopy vessels. Lymphatics stained with into...

Inflammatory stimulation of the liver induces nitric oxide (NO) biosynthesis and suppression detoxication. In this study effect NO on cytochrome P-450 (CYP) enzyme activity was investigated by comparing in vivo vitro assays. To establish inflammation, CD rats were injected with Corynebacterium parvum (C. parvum) suspension. After 5 days highly induced as indicated increased NO2- plus NO3- serum concentrations. At same time aminopyrine breath test (ABT), measuring CYP vivo, reduced to 42%...