A5066520318- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Aortic aneurysm repair treatments
- Cancer, Hypoxia, and Metabolism
- Protease and Inhibitor Mechanisms
- Proteoglycans and glycosaminoglycans research
- TGF-β signaling in diseases
- Inflammatory mediators and NSAID effects
- Atherosclerosis and Cardiovascular Diseases
- Renal and related cancers
- Coronary Interventions and Diagnostics
- Ovarian cancer diagnosis and treatment
- Aortic Disease and Treatment Approaches
- Antiplatelet Therapy and Cardiovascular Diseases
- Immune cells in cancer
- Nuclear Receptors and Signaling
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Blood Coagulation and Thrombosis Mechanisms
- Connective tissue disorders research
- Advanced Proteomics Techniques and Applications
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiac and Coronary Surgery Techniques
University of Washington
2010-2022
VA Puget Sound Health Care System
2008-2020
University of Washington Medical Center
2008-2017
Seattle University
2003-2013
United States Department of Veterans Affairs
2003-2011
University of Brescia
2008
Hope Heart Institute
2004
Augusta University
2004
Helsinki University Hospital
2004
The University of Texas MD Anderson Cancer Center
2000
Pericytes play an important role in modulating angiogenesis, but the origin of these cells is poorly understood. To evaluate whether mature vessel wall contains pericyte progenitor cells, nonendothelial mesenchymal isolated from rat aorta were cultured a serum-free medium optimized for stem cells. This method led to isolation anchorage-independent that proliferated slowly suspension, forming spheroidal colonies. process required basic fibroblast growth factor (bFGF) culture medium, because...
Abstract Hepatocellular carcinoma (HCC) is one of most malignant and aggressive human tumors. Transforming growth factor-β1 (TGF-β1) its coreceptor CD105 have been shown to contribute HCC progression. TGF-β1 also implicated in angiogenesis, but their role the vascularization has not investigated. To fill this gap, we studied effect on HCC-derived endothelium. By using immunomagnetic beads, isolated cultured endothelial cells (ECs) from (HCC-EC) adjacent nonneoplastic tissue (nNL-ECs)...
Vasculogenesis, the de novo formation of new blood vessels from undifferentiated precursor cells or angioblasts, has been studied with experimental in vivo and ex animal models, but its mechanism is poorly understood, particularly humans. We used aortic ring assay to investigate angioforming capacity explants 11- 12-week-old human embryos. After being embedded collagen gels, aorta rings produced branching capillary-like structures formed by mesenchymal spindle that lined a lumen expressed...
Background Tissues respond to injury by releasing acute phase reaction (APR) proteins which regulate inflammation and angiogenesis. Among the genes upregulated in wounded tissues are tumor necrosis factor-alpha (TNFα) reactant orosomucoid-1 (ORM1). ORM1 has been shown modulate response of immune cells TNFα, but its role on injury- TNFα-induced angiogenesis not investigated. This study was designed characterize angiogenic TNFα. Methods Results Angiogenesis studied with vitro, ex vivo, vivo...
Recent studies have implicated the Tie2 tyrosine-kinase receptor and its main ligands--angiopoietin-1 (Ang-1) angiopoietin-2 (Ang-2)--as crucial regulators of mural cell recruitment during angiogenesis. Angiopoietin-mediated activation promotes perivascular assembly, but mechanisms regulating this process are poorly understood because differentiated cells do not receptor, which is reportedly expressed only in endothelial cells. There also no direct evidence that results production...