A5033704196- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Microbial metabolism and enzyme function
- Biofuel production and bioconversion
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Heat shock proteins research
- Amino Acid Enzymes and Metabolism
- Metal-Catalyzed Oxygenation Mechanisms
- Biochemical Acid Research Studies
- Immune Response and Inflammation
- Computational Drug Discovery Methods
- Click Chemistry and Applications
- Biochemical and Molecular Research
- Biochemical and biochemical processes
- Infant Nutrition and Health
- Biocrusts and Microbial Ecology
- Photosynthetic Processes and Mechanisms
- S100 Proteins and Annexins
- Bacterial Genetics and Biotechnology
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
University of California, Davis
2014-2024
Brigham Young University–Hawaii
2014-2021
Abstract The ability to biosynthetically produce chemicals beyond what is commonly found in Nature requires the discovery of novel enzyme function. Here we utilize two approaches discover enzymes that enable specific production longer-chain (C 5 –C 8 ) alcohols from sugar. first approach combines bioinformatics and molecular modelling mine sequence databases, resulting a diverse panel capable catalysing targeted reaction. median catalytic efficiency computationally selected 75-fold greater...
Keto acid decarboxylase (Kdc) is a key enzyme in producing keto derived higher alcohols, like isobutanol. The most active Kdc’s are found mesophiles; the only reported Kdc activity thermophiles 2 orders of magnitude less active. Therefore, thermostability mesophilic limits isobutanol production temperature. Here, we report development thermostable 2-ketoisovalerate (Kivd) with 10.5-fold increased residual after 1h preincubation at 60 °C. Starting Lactococcus lactis Kivd, library was...
The rapidly growing appreciation of enzymes' catalytic and substrate promiscuity may lead to their expanded use in the fields chemical synthesis industrial biotechnology. Here, we explore enoyl-acyl carrier protein reductases (commonly known as FabI) how that is a function inherent reactivity geometric demands enzyme's active site. We demonstrate these enzymes catalyze reduction wide range substrates, particularly α,β-unsaturated aldehydes. In addition, combination quantum mechanical hydride...
To complement established rational and evolutionary protein design approaches, significant efforts are being made to utilize computational modeling the diversity of naturally occurring sequences. Here, we combine structural biology, genomic mining, identify features critical aldehyde deformylating oxygenases (ADOs), an enzyme family that has implications in synthetic biology chemoenzymatic synthesis. Through these efforts, discovered latent ADO-like function across ferritin-like superfamily...
Klebsiella pneumoniae contains an endogenous isobutanol synthesis pathway. The ipdC gene annotated as indole-3-pyruvate decarboxylase (Kp-IpdC), was identified to catalyze the formation of isobutyraldehyde from 2-ketoisovalerate.Compared with 2-ketoisovalerate Lactococcus lactis (KivD), a commonly used in artificial pathways, Kp-IpdC has 2.8-fold lower Km for 2-ketoisovalerate, leading higher production without induction. However, expression by IPTG induction resulted low titer. In vitro...
Introduction Historically, prioritizing abundant food production often resulted in overlooking nutrient quality and bioavailability, however, environmental concerns have now propelled sustainable nutrition health efficacy to the forefront of global attention. In fact, increasing demand for protein is major challenge facing system 21st century with an estimation that 70% more needed by 2050. This shift has spurred interest plant-based proteins their sustainability benefits, but most...
Enzymes have been through millions of years evolution during which their active-site microenvironments are fine-tuned. Active-site residues commonly conserved within protein families, indicating importance for substrate recognition and catalysis. In this work, we systematically mutated l-threonine dehydrogenase from Thermoplasma volcanium characterized the mutants against a panel analogs. Our results demonstrate that only subset these plays an essential role in catalysis native enzyme...
This study investigates whether selected WD40 proteins with a 7-bladed β-propeller structure, similar to that of the β subunit G protein heterotrimer, interact cytosolic chaperonin CCT and its known binding partner, PhLP1. Previous studies have shown is required for folding Gβ other proteins. The role PhLP1 in has also been established, but it unknown if assists Gβ-like three proteins, TBL2, MLST8 CDC20, PhLP1, was demonstrated this study. Co-immunoprecipitation assays identified one novel...
Previous studies have established that the cytosolic chaperonin complex, CCT and phosducin‐like protein (PhLP) are required for proper folding of G β subunit, assembly Gβγ subunit complex. The structure Gβ is a seven bladed, propeller. also shown has multiple binding partners, some which propeller structure, similar to Gβ. Our objective investigate if proteins with bladed would associate PhLP. We investigated interaction three Gβ‐like proteins, CDC20, TBL2 MLST8, Using co‐immunoprecipitation...
Abstract Background Klebsiella pneumoniae contains an endogenous isobutanol synthesis pathway. ipdC , annotated as indole-3-pyruvate decarboxylase (Kp-IpdC), was identified to catalyze the formation of isobutyraldehyde from 2-ketoisovalerate. Results Compared with 2-ketoisovalerate Lactococcus lactis (KivD), a commonly used in artificial synthesis, Kp-IpdC has 2.8-fold lower K m for 2-ketoisovalerate, leading higher production without induction. However, high level expression by induction...