A5038882936- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Acute Lymphoblastic Leukemia research
- Advanced Breast Cancer Therapies
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Chronic Myeloid Leukemia Treatments
- Galectins and Cancer Biology
- Acute Myeloid Leukemia Research
- CNS Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Viral-associated cancers and disorders
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Cytomegalovirus and herpesvirus research
- Cell Adhesion Molecules Research
- Genetics and Neurodevelopmental Disorders
- Circular RNAs in diseases
- Gastrointestinal Tumor Research and Treatment
- Gene expression and cancer classification
Central European Institute of Technology
2012-2025
Central European Institute of Technology – Masaryk University
2011-2025
Masaryk University
2012-2025
University Hospital Brno
2012-2025
Science for Life Laboratory
2016
Uppsala University
2015-2016
Ocní klinika
2015
Abstract High-grade B-cell lymphomas (HGBCLs) are aggressive blood cancers with a severe disease course, especially when the central nervous system (CNS) is involved. Standard histological examination depends on tissue availability and currently supplemented molecular tests, as status of MYC , BCL2 or BCL6 gene rearrangements required for proper lymphoma classification. This case report demonstrates relevance cerebrospinal fluid (CSF) cell-free DNA testing by integrative next-generation...
Next-generation sequencing has revealed novel recurrent mutations in chronic lymphocytic leukemia, particularly patients with aggressive disease. Here, we explored targeted re-sequencing as a strategy to assess the mutation status of genes prognostic potential. To this end, utilized HaloPlex enrichment technology and designed panel including nine genes: ATM, BIRC3, MYD88, NOTCH1, SF3B1 TP53, which have been linked prognosis KLHL6, POT1 XPO1, are less characterized but were found be...
ATM abnormalities are frequent in chronic lymphocytic leukemia and represent an important prognostic factor. Sole 11q deletion does not result inactivation by contrast to biallelic defects involving mutations. Therefore, the analysis of mutations their functional impact is crucial. In this study, we analyzed predominantly high-risk patients using: i) resequencing microarray direct sequencing; ii) Western blot for total level; iii) test based on p21 gene induction after parallel treatment...
Abstract Background Diagnosing primary or secondary CNS lymphoma (CNSL) is a clinical challenge due to the limitations of standard biopsy and imaging procedures despite established guidelines. Therefore, accurate biomarkers analytical methods that are convenient for practical routine use needed diagnose manage these aggressive lymphomas effectively. We evaluated utility minimally invasive circulating tumor DNA (ctDNA) detection in prospective real-world scenario, moving this approach closer...
Early identification of resistant cancer cells is currently a major challenge, as their expansion leads to refractoriness. To capture the dynamics these cells, we made comprehensive analysis disease progression and treatment response in chronic lymphocytic leukemia (CLL) patient using combination single-cell bulk genomic methods. At diagnosis, presented with unfavorable genetic markers, including notch receptor 1 (NOTCH1) mutation loss(11q). The initial subsequent lines did not lead durable...
B-cell neoplasms represent a clinically heterogeneous group of hematologic malignancies with considerably diverse genomic architecture recently endorsed by next-generation sequencing (NGS) studies. Because multiple genetic defects have potential or confirmed clinical impact, tendency toward more comprehensive testing diagnostic, prognostic, and predictive markers is desired. This study introduces the design, validation, implementation an integrative, custom-designed, capture-based NGS panel...
Recurrent mutations in splicing factor 3B subunit 1 (
MicroRNA (miRNA) expression is deregulated in many tumors including chronic lymphocytic leukemia (CLL). Although the particular mechanism(s) responsible for their aberrant not well characterized, presence of mutations and single-nucleotide polymorphisms (SNPs) miRNA genes, possibly affecting secondary structure expression, has been described. In CLL; however, impact frequency such variations have yet to be elucidated. Using a custom resequencing microarray, we screened sequence 109...
The ATM-p53 DNA-damage response (DDR) pathway has a crucial role in chemoresistance CLL, as indicated by the adverse prognostic impact of genetic aberrations TP53 and ATM. Identifying distinguishing ATM functional defects become relevant epigenetic posttranscriptional dysregulation ATM/p53 axis is increasingly being recognized underlying cause chemoresistance. Also, specific treatments sensitizing TP53- or ATM-deficient CLL cells are emerging. We therefore developed new assay with aim to (i)...
Molecular profiling of tumor samples has acquired importance in cancer research, but currently also plays an important role the clinical management patients. Rapid identification genomic aberrations improves diagnosis, prognosis and effective therapy selection. This can be attributed mainly to development next-generation sequencing (NGS) methods, especially targeted DNA panels. Such panels enable a relatively inexpensive rapid analysis various with impact specific particular diagnoses. In...
Introduction BCR-ABL1-like acute lymphoblastic leukemia (ALL) is a high-risk disease with complex genomic background. Though extensively studied, data on the frequency and mutual associations of present mutations are still incomplete in adult patients. This retrospective study aims to map landscape B-other ALL cohort patients focus subtype.Methods We analyzed bone marrow peripheral blood samples treated consecutively at three major Czech teaching hospitals. Samples were by cytogenetic...
The prognostic role of ATM defects is well documented in chronic lymphocytic leukemia. However, the predictive value inactivation much less understood, even response to common drugs like fludarabine. It has been demonstrated that CLL cells having inactive exhibit defective phosphorylation its downstream targets after fludarabine treatment. We performed alternative analysis focusing on fludarabine-induced p53 accumulation and induction p53-downstream genes artificial inhibition and, parallel,...
Abstract Near‐haploid acute lymphoblastic leukemia is rare subgroup of the disease, which very important due to poor prognosis and resistance treatment including novel monoclonal antibodies CAR‐T therapy.
Telomeres are protective structures at chromosome ends which shorten gradually with increasing age. In chronic lymphocytic leukemia (CLL), short telomeres have been associated unfavorable disease outcome, but the link between clonal evolution and telomere shortening remains unresolved.We investigated relative length (RTL) in a well-characterized cohort of 198 CLL patients by qPCR focused detail on subgroup 26 who underwent TP53 mutations (evolTP53). evolTP53 we explored factors influencing...