A5004704148- Mitochondrial Function and Pathology
- Ion channel regulation and function
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Genetic Neurodegenerative Diseases
- Inflammasome and immune disorders
- Photosynthetic Processes and Mechanisms
- Lysosomal Storage Disorders Research
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Genetics and Neurodevelopmental Disorders
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Cardiac electrophysiology and arrhythmias
- Chemical synthesis and pharmacological studies
- RNA Research and Splicing
- Influenza Virus Research Studies
- Cancer-related Molecular Pathways
- Immune Cell Function and Interaction
- Magnesium Alloys: Properties and Applications
- Ferrocene Chemistry and Applications
- Synthesis of heterocyclic compounds
- Cytokine Signaling Pathways and Interactions
Karl Landsteiner University of Health Sciences
2022-2024
Max Delbrück Center
2017-2022
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2020-2021
University of Salzburg
2012-2014
The serine/threonine protein phosphatase 1 (PP1) dephosphorylates hundreds of key biological targets by associating with nearly 200 regulatory proteins to form highly specific holoenzymes. However, how these direct PP1 specificity and the ability predict interacting bind from sequence alone is still missing. nuclear targeting subunit (PNUTS) a that, PP1, plays central role in nucleus, where it regulates chromatin decondensation, RNA processing, phosphorylation state fundamental cell cycle...
The mitochondrial contact site and cristae organizing system (MICOS) is crucial for the formation of crista junctions inner membrane architecture. MICOS contains two core components. Mic10 shows membrane-bending activity, whereas Mic60 (mitofilin) forms sites between outer membranes. Here we report that deforms liposomes into thin tubules thus displays membrane-shaping activity. We identify a membrane-binding in soluble intermembrane space-exposed part Mic60. This formed by predicted...
Human dynamin-1-like protein (DNM1L) is a GTP-driven molecular machine that segregates mitochondria and peroxisomes. To obtain insights into its catalytic mechanism, we determined crystal structures of construct comprising the GTPase domain bundle signaling element (BSE) in nucleotide-free GTP-analogue-bound states. The DNM1L structurally related to dynamin binds nucleotide 5′-Guanylyl-imidodiphosphate (GMP-PNP) via five highly conserved motifs, whereas BSE folds pocket at opposite side....
Abstract Vesicular traffic and membrane contact sites between organelles enable the exchange of proteins, lipids, metabolites. Recruitment tethers to endoplasmic reticulum (ER) plasma is often triggered by calcium. Here we reveal a function for calcium in repression cholesterol export at ER Golgi complex. We show that efflux from stores induced inositol-triphosphate [IP 3 ] accumulation upon loss inositol 5-phosphatase INPP5A or receptor signaling triggers depletion associated Gb3 cell...
Mitochondrial cristae membranes are the oxidative phosphorylation sites in cells. Crista junctions (CJs) form highly curved neck regions of and thought to function as selective entry gates into space. Little is known about how CJs generated maintained. We show that central coiled-coil (CC) domain mitochondrial contact site organizing system subunit Mic60 forms an elongated, bow tie–shaped tetrameric assembly. Mic19 promotes tetramerization via a conserved interface between mitofilin CHCH...
NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family cytosolic receptors an inflammation signaling cascade. Here, we present crystal structure pyrin domain (PYD) at 2.3 Å resolution. The PYD death (DD) superfamily adopts DD fold consisting six α-helices tightly packed around hydrophobic core, with highly charged surface that typical PYDs. Importantly, however, identified several differences between other structures are significant enough to affect...
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Acute myeloid leukemia (AML) is a heterogenous disease characterized by the clonal expansion of progenitor cells. Despite recent advancements in treatment AML, relapse still remains significant challenge, necessitating development innovative therapies to eliminate minimal residual disease. One promising approach address these unmet clinical needs natural killer (NK) cell immunotherapy. To implement such treatments effectively, it vital comprehend how AML cells escape NK-cell surveillance....
The cytosolic tripartite NLR receptors serve as important signalling platforms in innate immunity. While the C-terminal domains act sensor and activation modules, N-terminal death-like domain, e.g. CARD or pyrin is thought to recruit downstream effector molecules by homotypic interactions. Such complexes have been determined for all members of death-domain superfamily except domains. Here, crystal structures human NLRP14 pyrin-domain variants are reported. wild-type protein well clinical...
Background: α2δ proteins regulate membrane trafficking and biophysical properties of voltage-gated calcium channels. Moreover, they modulate axonal wiring, synapse formation, trans-synaptic signaling. Several rare missense variants in CACNA2D1 (coding for α2δ-1) CACNA2D3 α2δ-3) genes were identified patients with autism spectrum disorder (ASD). However, the pathogenicity these is not known, molecular mechanism by which may contribute to pathophysiology is, as today, understood. Therefore,...
Abstract Mitochondrial cristae membranes are the oxidative phosphorylation sites in cells. Crista junctions (CJs) form highly curved neck regions of and thought to function as selective entry gates into space. Little is known about how CJs generated maintained. We show that central coiled-coil domain mitochondrial contact organizing system (MICOS) subunit Mic60 forms an elongated, bow tie-shaped tetrameric assembly. Mic19 promotes tetramerization via a conserved interface between mitofilin...
<title>Abstract</title> Vesicular traffic and membrane contact sites between organelles enable the exchange of proteins, lipids, metabolites. Recruitment tethers to endoplasmic reticulum (ER) plasma is often triggered by calcium. In contrast, we reveal here a function for calcium in repression cholesterol export at ER Golgi complex. We show that efflux from stores induced inositol-triphosphate [IP3] accumulation upon loss inositol 5-phosphatase INPP5A or sustained receptor signaling triggers...