A5011402503- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- T-cell and B-cell Immunology
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Cytokine Signaling Pathways and Interactions
- Gut microbiota and health
- Ubiquitin and proteasome pathways
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- Immune cells in cancer
- Multiple Myeloma Research and Treatments
- Neutropenia and Cancer Infections
- NF-κB Signaling Pathways
- Phagocytosis and Immune Regulation
- Nanoplatforms for cancer theranostics
Universidad de Zaragoza
2015-2025
Hospital Clínico Universitario Lozano Blesa
2016-2024
Instituto de Investigación Sanitaria Aragón
2015-2024
Instituto de Nanociencia y Materiales de Aragón
2015-2024
Consejo Superior de Investigaciones Científicas
2021-2024
Instituto Aragonés de Ciencias de la Salud
2016-2021
University College London
2018
Instituto de Salud Carlos III
2017
Universidad de Extremadura
2016
Immunité et Cancer
2015
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity biochemical environment (such as pH and ionic strength) rapid yet not well-understood heat transfer mechanisms throughout cell. Here, we present unique system for intracellular temperature mapping fluorescence microscope (uncertainty 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated breast cancer MDA-MB468 cells. Two-dimensional (2D)...
Background: Tumor necrosis factor is a crucial proinflammatory cytokine in immune-mediated diseases. inhibitors (TNFi), such as infliximab and adalimumab, effectively treat rheumatological digestive disorders. However, challenges primary nonresponse, secondary treatment failure, or adverse reactions limit their efficacy. Monitoring TNFi levels essential for optimizing improving outcomes. An enzyme-linked immunosorbent assay (ELISA; Promonitor) was compared with 2 commercially available...
We previously observed that T lymphocytes present in synovial fluid (SF) from patients with rheumatoid arthritis (RA) were sensitive to APO2L/TRAIL. In addition, there was a drastic decrease the amount of bioactive APO2L/TRAIL associated exosomes SF RA patients. This study undertaken evaluate effectiveness conjugated artificial lipid vesicles resembling natural as treatment rabbit model antigen-induced (AIA).We used novel Ni(2+)-(N-5-amino-1-carboxypentyl)-iminodiacetic acid)-containing...
Human Apo2-ligand/TRAIL is a member of the TNF cytokine superfamily capable inducing apoptosis on tumor cells while sparing normal cells. Besides its antitumor activity, Apo2L/TRAIL also implicated in immune regulation. stored inside activated T cytoplasmic multivesicular bodies and physiologically released to extracellular medium inserted internal membrane vesicles, known as exosomes. In this study we have generated artificial lipid vesicles coated with bioactive Apo2L/TRAIL, which resemble...
If not properly regulated, the inflammatory immune response can promote carcinogenesis, as evident in colorectal cancer (CRC). Aiming to gain mechanistic insight into link between inflammation and CRC, we perform transcriptomics analysis of human identifying a strong correlation expression serine protease granzyme A (GzmA) inflammation. In dextran sodium sulfate azoxymethane (DSS/AOM) mouse model, deficiency pharmacological inhibition extracellular GzmA both attenuate gut prevent CRC...
Background Lung ultrasound is feasible for assessing lung injury caused by coronavirus disease 2019 (COVID-19). However, the prognostic meaning and time-line changes of assessed in COVID-19 hospitalised patients are unknown. Methods Prospective cohort study designed to analyse value using a quantitative scale (lung Zaragoza (LUZ)-score) during first 72 h after admission. The primary end-point was in-hospital death and/or admission intensive care unit. Total length hospital stay, increase...
COVID-19 pneumonia is often associated with hyperinflammation. The efficacy and safety of anakinra in treating patients severe hyperinflammation are still unclear.To assess the vs standard care alone for hyperinflammation.The Clinical Trial Use Anakinra Cytokine Storm Syndrome Secondary to (ANA-COVID-GEAS) was a multicenter, randomized, open-label, 2-group, phase 2/3 clinical trial conducted at 12 hospitals Spain between May 8, 2020, March 1, 2021, follow-up 1 month. Participants were adult...
Global DNA hypomethylation in CD4(+) T cells has been detected systemic lupus erythematosus (SLE) and it seems to be linked its pathogenesis. We investigated the relationship between overall methylation expression of three (cytosine-5) methyltransferases involved process. The deoxymethylcytosine (dmC) content purified from 29 SLE patients 30 healthy controls was measured by means an enzyme-linked immunosorbent assay (ELISA). transcript levels cytosine-5-methyltransferase 1 (DNMT1), 3A...
Abstract Global DNA hypomethylation in CD4+ T cells has been detected systemic lupus erythematosus (SLE), and it seems to be linked its pathogenesis. We investigated the relationship between overall methylation expression of two methyl CpG-binding domain (MBD) proteins. deoxymethylcytosine (dmC) content purified from 29 SLE patients 30 healthy controls was measured by means an ELISA. Transcript levels proteins (MBD2 MBD4) were quantified real-time RT-PCR. Association studies also carried out...
// Alberto Bosque 1, 4, * , Lisa Dietz 2, Ana Gallego-Lleyda Manuel Sanclemente 1 María Iturralde Javier Naval Angeles Alava Luis Martínez-Lostao 3, 5, # Hermann-Josef Thierse Anel Departamento de Bioquímica y Biología Molecular Celular, Facultad Ciencias, Universidad Zaragoza, Instituto Investigación Sanitaria Aragón (IIS-Aragón), Spain 2 Research Group for Immunology & Proteomics, Department of Dermatology, University Medical Center Mannheim, Ruprechts-Karls-University, Heidelberg, Germany...
(1) Despite the effectiveness of immune checkpoint inhibitors (ICIs) in lung cancer, there is a lack knowledge about predictive biomarkers. The objective our study to analyze different subsets T-lymphocytes and natural killer (NK) cells as biomarkers cohort patients with nonsmall cell cancer (NSCLC) treated ICI. (2) This an observational, prospective 55 NSCLC A total 43 T NK are analyzed peripheral blood, including main markers exhaustion, differentiation, memory, activation, inhibition. (3)...
Immune checkpoint inhibitors have been proposed as the standard treatment for different stages of non-small-cell lung cancer in multiple indications. Not all patients benefit from these treatments, however, and certain develop immune-related adverse events. Although search predictors response to drugs is a major field research, issues yet be resolved. It has postulated that microbiota could play relevant role conditioning treatments; human factor intestinal permeability also needs considered...