A5042584393- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Gynecological conditions and treatments
- Pluripotent Stem Cells Research
- Cancer Genomics and Diagnostics
- Retinoids in leukemia and cellular processes
- Sarcoma Diagnosis and Treatment
- Reproductive System and Pregnancy
- Cancer Mechanisms and Therapy
- Peroxisome Proliferator-Activated Receptors
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Lipoproteins and Cardiovascular Health
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Bone health and treatments
- Ovarian cancer diagnosis and treatment
- Cancer Cells and Metastasis
- Uterine Myomas and Treatments
- Renal and related cancers
- PI3K/AKT/mTOR signaling in cancer
Gladstone Institutes
2024
Kyoto University
2023-2024
Keio University Hospital
2022
Keio University
2016-2020
Abstract Recently, several studies using cultures of human embryos together with single-cell RNA-seq analyses have revealed differences between humans and mice, necessitating the study 1–8 . Despite importance embryology, ethical legal restrictions limited post-implantation-stage studies. Thus, recent efforts focused on developing in vitro self-organizing models stem cells 9–17 Here, we report genetic non-genetic approaches to generate authentic hypoblast (naive hPSC-derived hypoblast-like...
Drug repositioning is an alternative strategy redirecting existing drugs for new disease. We have previously reported antitumor effect of statins, antidyslipidemic drugs, on ovarian cancer in vitro and vivo. In this study, we investigated the effects other mevalonate pathway inhibitors mechanism from a metabolic perspective. The tumor cell growth were evaluated vitro. Bisphosphonates that inhibit are commonly used as antiosteoporotic bisphosphonate examined Metabolites SKOV3 cells analyzed...
Objective: With the emerging significance of genetic profiles in management endometrial cancer, identification tumor-driving genes with prognostic value is a pressing need.The LAMC1 gene, encoding laminin subunit gamma 1 (LAMC1) protein, has been reported to be involved progression various malignant tumors.In this study, we aimed investigate role cancer and elucidate underlying mechanism.Methods: We evaluated immunohistochemical expression atypical hyperplasia cancer.Within cases, analyzed...
The increasing incidence of obesity and diabetes due to changes in diet, earlier menarche, delayed menopause, late marriage, declining birth rate have resulted an increase the number endometrial cancer cases over last few decades. Although surgical therapy is sufficient for early cancer, there no effective patients with advanced recurrent cancer. oncogenic mechanism involves microsatellite instability (MSI) caused by dysfunction DNA mismatch repair genes 30% patients. Immune checkpoint...
Tumors comprise heterogeneous cell types including cancer stem cells (CSC), progenitor cells, and differentiated cells. Chemoresistance is a potential cause of relapse key characteristic CSC, but the development novel therapeutic approaches for targeting these has been limited. We previously established osteosarcoma-initiating (OSi) by introducing gene c-Myc into bone marrow stromal Ink4a/Arf knockout mice. These OSi are composed two distinct clones: highly tumorigenic (AX cells), similar to...
Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to destabilizing domain (H1FOO-DD), together with OCT4, SOX2, KLF4, LMYC, in human somatic cells improves quality of reprogramming pluripotency. H1FOO-DD expression was associated altered chromatin accessibility around pluripotency genes suppression innate immune response. Notably, generates induced lower...
Abstract Adipocyte differentiation is accompanied by a pronounced change in the actin cytoskeleton characterized reorganization of filamentous (F)‐actin stress fibers into cortical F‐actin structures. We previously showed that depolymerization induced inactivation RhoA–ROCK (Rho‐associated kinase) signaling acts as trigger for adipocyte differentiation. The relevance and underlying mechanism formation structures from depolymerized during have remained unclear, however. now examined...
Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS) is a disease caused by congenital absence of the uterus and two-thirds upper vagina. The pathogenic mechanism MRKHS may involve gene abnormalities, there are various case reports associating with Wnt family member 4 (Wnt4) mutation. Analysis genes mapped to regions in which deletion duplication frequently detected patients has shown involvement LIM homeobox 1 (LHX1), HNF1 B (HNF1B) T-box 6 (TBX6). In addition, chromosomal translocation...
Germline mutation of DNA mismatch repair (MMR) genes is a cause Lynch syndrome. Methylation MutL homolog 1 (MLH1) and MutS 2 (MSH2) has been detected in peripheral blood cells patients with colorectal cancer. This methylation referred to as epimutation. these not studied an unselected series endometrial cancer cases. Therefore, we examined MLH1, MSH2, MSH6 promoter regions 206 using methylation-specific polymerase chain reaction (MSP). MMR genes, microsatellite instability (MSI),...
In the field of drug repurposing, use statins for treating dyslipidemia is considered promising in ovarian cancer treatment based on epidemiological studies and basic research findings. Biomarkers should be established to identify patients who will respond statin achieve clinical application. present study, we demonstrated that have a multifaceted mode action involve pathways other than protein prenylation. To biomarkers predict response statins, subjected cells microarray analysis...
Endometrial cancer in the lower uterine segment (LUS) is associated with Lynch syndrome MLH1 or MSH2 germline mutation. Here, we report a case of carcinoma LUS diagnosed based on MSH6 mutation 46-year-old woman abnormal vaginal bleeding. She had rectal at age 39 family history colon (father, 75 years), pancreatic (paternal grandmother, 74 and (maternal 85 years). Magnetic resonance imaging showed tumor LUS. biopsy revealed endometrioid adenocarcinoma G1. As her met revised Bethesda criteria,...
Background . Surgery for gynecologic cancer with lymphadenectomy and pelvic radiotherapy can produce lymphoceles that sometimes complicate infection, resulting in abscesses. The true pathogenic bacteria of abscesses are not always found because false-negative results due to administered antibiotics difficulty detection, including anaerobic bacteria. Analyzing flora by next-generation sequencing (NGS) using 16S ribosomal DNA may reveal the This is first report on causative pathogens...
To date, only few large studies are available concerning the safety and diagnostic concordance rates of outpatient flexible hysteroscopy. In our institution, hysteroscopy has been routinely educationally applied Kosuke Tsuji to intrauterine lesions; thus, we retrospectively investigated institution's cases.A total 1591 cases conducted at institution in 2012-2016 were analyzed terms their clinical background, complications rates.A included 546 benign tumors (317 endometrial polyps, 168 myomas...
Angiosarcoma is a rare malignant tumor with an aggressive clinical course and poor prognosis. Intraperitoneal angiosarcoma, especially originating from the omentum, extremely rare. We report case of radiation-induced angiosarcoma omentum that arose in 38-year-old female seven years after concurrent chemoradiotherapy for cervical cancer. The primary was unknown until diagnostic laparoscopy revealed unresectable omental mass. Pathological examination high-grade cells positive endothelial...
<p>List of anticancer agents tested in the experiment shown Supplementary Figure S19.</p>
<p>Supplementary Figure Legends</p>
<p>Supplementary Fig. S10 Knockdown of either ROCK1 or ROCK2 alone induces terminal adipocyte differentiation and growth suppression AO cells. Supplementary S11 Effect chemical agents that induce depolymerization the actin cytoskeleton on in S12 Immunohistochemical staining for MKL1 an array human osteosarcoma specimens. S13 Effects induction a constitutively active mutant expression target genes S14 fasudil treatment mice injected with S15 heterogeneous S16 sequential doxorubicin...
<p>Supplementary Fig. S1 Differentiation potential of AO and AX cells. Supplementary S2 High Intrinsic efflux capacity contributes to the chemoresistance S3 Immunofluorescence microscopy FABP4 PLIN1 expression in heterogeneous osteosarcoma S4 Effect doxorubicin on PPARG, a master regulator adipogenesis, human cell lines. S5 AO-like cells expressing PPARG are present recurrent tissue but not parosteal tissue. S6 Treatment with fasudil alone induces terminal adipocyte differentiation S7...
<p>Supplementary Materials and Methods, Supplementary References</p>
<div>Abstract<p>Tumors comprise heterogeneous cell types including cancer stem cells (CSC), progenitor cells, and differentiated cells. Chemoresistance is a potential cause of relapse key characteristic CSC, but the development novel therapeutic approaches for targeting these has been limited. We previously established osteosarcoma-initiating (OSi) by introducing gene c-Myc into bone marrow stromal <i>Ink4a/Arf</i> knockout mice. These OSi are composed two distinct...