A5007023070- Immune cells in cancer
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Caveolin-1 and cellular processes
- Immune Cell Function and Interaction
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gestational Diabetes Research and Management
- S100 Proteins and Annexins
- Pancreatic and Hepatic Oncology Research
- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Anesthesia and Pain Management
- Maternal and Perinatal Health Interventions
- Planetary Science and Exploration
- Resilience and Mental Health
- interferon and immune responses
- Suicide and Self-Harm Studies
- Sphingolipid Metabolism and Signaling
- Fibroblast Growth Factor Research
- Plant and Fungal Interactions Research
La Jolla Institute for Immunology
2019-2024
John Brown University
2024
Technische Universität Berlin
2023
Cardiff University
2022
Park Plaza Hospital
2022
UW Health University Hospital
2019-2021
Anna Needs Neuroblastoma Answers
2020
APA Corporation (United States)
2019
Texas A&M University
2019
Washington University in St. Louis
2014-2018
Abstract Cancer immunotherapy generally offers limited clinical benefit without coordinated strategies to mitigate the immunosuppressive nature of tumor microenvironment. Critical drivers immune escape in microenvironment include tumor-associated macrophages and myeloid-derived suppressor cells, which not only mediate suppression, but also promote metastatic dissemination impart resistance cytotoxic therapies. Thus, ablate effects these myeloid cell populations may offer great therapeutic...
Tumors employ multiple mechanisms to evade immune surveillance. One mechanism is tumor-induced myelopoiesis, whereby the expansion of immunosuppressive myeloid cells can impair tumor immunity. As and conventional dendritic (cDCs) are derived from same progenitors, we postulated that myelopoiesis might impact cDC development. The subset, cDC1, which includes human CD141+ DCs mouse CD103+ DCs, supports anti-tumor immunity by stimulating CD8+ T-cell responses. Here, understand how cDC1...
Tumor–stroma interactions contribute to tumorigenesis. Tumor cells can educate the stroma at primary and distant sites facilitate recruitment of heterogeneous populations immature myeloid cells, known as myeloid-derived suppressor (MDSCs). MDSCs suppress T cell responses promote tumor proliferation. One outstanding question is how local modulate during progression. Down-regulation β-catenin critical for MDSC accumulation immune suppressive functions in mice humans. Here, we demonstrate that...
Tumor-infiltrating myeloid cells contribute to the development of immunosuppressive tumor microenvironment. Myeloid cell expression arginase 1 (ARG1) promotes a protumor phenotype by inhibiting T function and depleting extracellular l-arginine, but mechanism underlying this expression, especially in breast cancer, is poorly understood. In cancer clinical samples our mouse models, we identified tumor-derived GM-CSF as primary regulator ARG1 local immune suppression through gene-KO screen...
Advances in the field of cancer immunology, including studies on tumor-infiltrating CD8+ cytotoxic T lymphocytes (CTLs), have led to new immunotherapeutics with proven efficacy against late-stage cancers. However, antitumor potential immune system targeting early-stage cancers remains uncertain. Here, we demonstrated that both genetic and chemical induction thymic stromal lymphopoietin (TSLP) at a distant site leads robust immunity spontaneous breast carcinogenesis mice. Breast tumors...
Abstract Integrin β3 is critical for tumor invasion, neoangiogenesis, and inflammation, making it a promising cancer target. However, preclinical clinical data of integrin antagonists have demonstrated no benefit or worse outcomes. We hypothesized that could affect immunity evaluated tumors in mice with deletion macrophage lineage cells (β3KOM). β3KOM had increased melanoma breast growth tumor-promoting M2 macrophages decreased CD8+ T cells. antagonist, cilengitide, also enhanced function....
Abstract The role of nonclassical, patrolling monocytes in lung tumor metastasis and their functional relationships with other immune cells remain poorly defined. Contributing to these gaps knowledge is a lack cellular specificity commonly used approaches for depleting nonclassical monocytes. To circumvent limitations study the melanoma lungs, we generated C57BL/6J mice which Nr4a1 superenhancer E2 subdomain ablated (E2−/− mice). E2−/− but preserve classical monocyte macrophage numbers...
CD8 + T cells drive anti-cancer immunity in response to antigen-presenting such as dendritic and subpopulations of monocytes macrophages. While CD14 classical modulate cell responses, the contributions CD16 nonclassical this process remain unclear. Herein we explored role activation by utilizing E2-deficient (E2 -/- ) mice that lack monocytes. During early metastatic seeding, modeled B16F10-OVA cancer injected into E2 mice, noted lower effector memory frequencies within lungs well...
Abstract: Stress‐activated protein kinase (SAPK) and extracellular signal‐regulated (ERK), both members of the mitogen‐activated (MAPK) family, may in some circumstances serve opposing functions with respect to cell survival. However, SAPK ERK can also be coordinately activated neurons response glutamate stimulation NMDA receptors. To explore mechanisms these MAPK activations, we compared ionic mediating activations by glutamate. In primary cultures striatal neurons, glutamatergic activation...
MDSCs comprise a heterogeneous population of immature Gr1 + /CD11b cells in mice and CD33 humans (Gabrilovich et al., 2012).This myeloid is further classified into granulocytic or monocytic based on the expression levels Ly6G Ly6C, respectively, mouse model CD15 CD14 humans.Investigations mechanisms that drive MDSC recruitment activity have shown GM-CSF, IL-6, VEGF play an important role via modulation Jak-STAT signaling pathways 2001;Trikha Carson, 2014).In addition to Jak-STAT, we recently...
Background and Objectives Leukapheresis of non‐mobilized healthy donors is performed to harvest monocytes lymphocyte subpopulations for use in various therapeutic regimens. In this methodological study, we compared two different leukapheresis programs, using equivalent volumes processed blood over similar processing periods, determine the influence procedures on donor peripheral count establish procedure that yields highest quality product. Materials Methods The target variables obtained 41...
Purpose Due to their abundance in the blood, low RNA content, and short lifespan, neutrophils have been classically considered be one homogenous pool. However, recent work has found that mature neutrophil progenitors are composed of unique subsets exhibiting context-dependent functions. In this study, we ask if heterogeneity is associated with melanoma incidence and/or disease stage. Experimental design Using mass cytometry, profiled patient blood for cell surface markers among neutrophils....
TRIM5α (tripartite motif 5α) acts as a pattern recognition receptor specific for the retrovirus capsid lattice and blocks infection by HIV-1 immediately after entry. However, precise mechanisms underlying this rapid of viral components remain elusive. Here, we analyzed influence exposure on TRIM5α. Total internal reflection fluorescence microscopy lipid flotation assays revealed recruitment subpopulation to plasma membrane (PM) upon vesicular stomatitis virus-G-pseudotyped viral-like...