A5090684529- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Pharmacogenetics and Drug Metabolism
- Analytical Chemistry and Chromatography
- Radiopharmaceutical Chemistry and Applications
- Enzyme Structure and Function
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- HIV Research and Treatment
- Plant Virus Research Studies
- Drug Transport and Resistance Mechanisms
- HER2/EGFR in Cancer Research
- Immune Cell Function and Interaction
- Mass Spectrometry Techniques and Applications
Catalent (United States)
2018-2019
Sangamo BioSciences (United States)
2006-2010
Point Richmond Tech Center
2008-2010
Scripps Research Institute
2010
Isogenic settings are routine in model organisms, yet remain elusive for genetic experiments on human cells. We describe the use of designed zinc finger nucleases (ZFNs) efficient transgenesis without drug selection into PPP1R12C gene, a “safe harbor” locus known as AAVS1 . ZFNs enable targeted at frequency up to 15% following transient transfection both transformed and primary cells, including fibroblasts hES When added this locus, transgenes such expression cassettes shRNAs,...
P450cam from Pseudomonas putida is the best characterized member of vast family cytochrome P450s, and it has long been believed to have a more rigid closed active site relative other P450s. Here we report X-ray structures crystallized in absence substrate at high low [K(+)]. The camphor-free are observed distinct open conformation by water-filled channel created retraction F G helices, disorder B' helix, loss K(+) binding site. Crystallization presence alone does not alter conformation,...
Oncology treatment has been revolutionized by the introduction of immune checkpoint inhibitor drugs, which enable 20–40% patients to generate anti-tumor responses. Combination approaches with chemotherapeutic drugs may responses in remaining patient cohorts. In this regard, a handful are promising due their ability induce immunogenic cell death target cells. However, these agents systemically delivered and indiscriminately cytotoxic proliferating By contrast, antibody-drug conjugates can...
The advantages of site-specific over stochastic bioconjugation technologies include homogeneity product, minimal perturbation protein structure/function, and – increasingly the ability to perform structure activity relationship studies at conjugate level. When selecting optimal location for payload placement, many researchers turn in silico modeling identify regions predicted offer solvent-exposed conjugatable sites while conserving function. Here, using aldehyde tag as our technology...
Zinc finger nucleases have been shown to be effective tools for high-frequency genome editing with potential therapeutic applications (Urnov et al., Nature 435:646-651). Such are created by fusing the cleavage domain of type IIs restriction enzyme Fok I engineered zinc proteins. One limitation this architecture is that natural must dimerize in order cleave DNA; therefore two different protein-Fok fusions required introduce a double-stranded break at an asymmetric target site. As consequence,...