A5091375379- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Parasitic Infections and Diagnostics
- Herpesvirus Infections and Treatments
- Science Education and Pedagogy
- Viral Infections and Vectors
- Malaria Research and Control
- Sepsis Diagnosis and Treatment
- Chemistry Education and Research
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Science and Education Research
- Streptococcal Infections and Treatments
- Travel-related health issues
- Parvovirus B19 Infection Studies
Universidade Federal do Pampa
2020
Instituto de Medicina Tropical
2013-2019
Universidade de São Paulo
2013-2018
Fundação de Amparo à Pesquisa do Estado de São Paulo
2017
Hospital São Paulo
2017
Toxoplasmosis may be life-threatening in fetuses and immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence IgM IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim this study was compare performance 4 PCR-based methods for toxoplasmosis. One hundred pregnant women who seroconverted during pregnancy were included study. definition cases a 12-month...
Bacterial sepsis remains a major cause of mortality and blood cultures are the gold standard laboratory diagnosis even though they lack sensitivity in neonates. Culturenegative sepsis, also known as clinical has long been considered neonatal intensive care units because, well culture-positive infants, culture-negative neonates have worse prognosis comparison with non-infected ones. Quantitative amplifications used to detect bacterial infections but results only qualitative way (positive or...
To correlate neonatal and infant clinical outcome with parasite load in amniotic fluid (AF).We conducted a retrospective cohort study of 122 children whose mothers had toxoplasmosis during pregnancy. The were monitored from birth to 12 months old. Stored AF samples obtained at maternal diagnosis tested by quantitative polymerase chain reaction. Gestational age infection, reaction results, anti-Toxoplasma gondii immunoglobulin (Ig) M, correlated.Maternal infection occurred 18 (14.7%) 104...
Host and parasite genotypes are among the factors associated with congenital toxoplasmosis pathogenesis. As HLA class II molecules play a key role in immune system regulation, aim of this study was to investigate whether HLA-DQA1/B1 alleles susceptibility or protection toxoplasmosis. One hundred twenty-two fetuses 103 without were studied. The two groups comparable according number socio-demographic genetic variables. typed by PCR-SSP. In HLA-DQA1 region, allele frequencies showed that...
Only a small percentage of individuals living in endemic areas develop severe malaria suggesting that host genetic factors may play key role. This study has determined the frequency single nucleotide polymorphisms (SNPs) some pro and anti-inflammatory cytokine gene sequences: IL6 (-174; rs1800795), IL12p40 (+1188; rs3212227), IL4 (+33; rs2070874), IL10 (-3575; rs1800890) TGFb1 (+869; rs1800470), by means PCR-RFLP. Blood samples were collected from 104 symptomatic 37 asymptomatic subjects....
Objective: To monitor the bacterial load in newborns with proven infections on day of admission, 48 h and 7 days after treatment.
Context.— Infections are the leading cause of perinatal and infant mortality in low-income low-resource countries, which have a higher prevalence infections. Definitive diagnosis congenital infections is largely dependent upon results laboratory tests. Objective.— To develop multiplex nested polymerase chain reaction (PCR) technique for simultaneous detection 7 pathogens containing DNA their genomes suspected cases infection. Design.— Eligible participants were pregnant women with positive...
Concept mapping consist of diagrams that represent the relationships between concepts and words describe them. The present study aimed to examination student perceptions using concept in a pharmacy course. A group undergraduate students from sixth semester were participants. Students received before after theoretical class, also could make their own map. In general manner, 97% made use mapping, 90% consider may be useful for learning pharmacology. 50% participants considered help provided by...