A5053895726- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Skin and Cellular Biology Research
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Psoriasis: Treatment and Pathogenesis
- Toxin Mechanisms and Immunotoxins
- Skin Protection and Aging
- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Complement system in diseases
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- HER2/EGFR in Cancer Research
- Circadian rhythm and melatonin
- Glycosylation and Glycoproteins Research
- Silk-based biomaterials and applications
- Redox biology and oxidative stress
- Wnt/β-catenin signaling in development and cancer
- IL-33, ST2, and ILC Pathways
Fukushima Medical University
2001-2024
Yamagata University
2011-2019
University of Washington
2006-2009
Seattle University
2006-2009
Imperial Household Agency
1998-2004
Hirosaki University
2002
Institute of Biomedical Science
2001
Tokyo Metropolitan Institute of Gerontology
1995-1996
The University of Tokyo
1989-1994
Tokyo University of Science
1989-1994
Abstract Cross-linking of the B cell Ag receptor (BCR) induces tyrosine phosphorylation multiple cellular substrates, including phospholipase C (PLC)-γ2, which is involved in activation phosphatidylinositol pathway. To assess importance PLC-γ2 murine lymphopoiesis, gene was inducibly ablated by using IFN-regulated Cre recombinase. Mice with a neonatally induced loss function displayed reduced numbers mature conventional cells and peritoneal B1 defective responses vitro to BCR stimulation...
To identify mAb reacting with the HLA class I alpha 3 domain, 14 recognizing monomorphic determinants expressed on HLA-A, B, and C Ag or restricted to HLA-B were screened in indirect immunofluorescence mouse L cells expressing HLA-B7/H-2Kb chimeric Ag. CR1S63, CR10-215, CR11-115, W6/32 found react domain addition 2 domain. Q1/28 TP25.99 only The recognized by six mapped staining of H-2Kb containing different segments HLA-B7 chimerized reacts 184 199 stretch, CR10-215 CR11-115 246 CR1S63 256...
A basic beta-galactosidase (beta-Galase) has been purified 281-fold from imbibed radish (Raphanus sativus L.) seeds by conventional purification procedures. The enzyme is an electrophoretically homogeneous protein consisting of a single polypeptide with apparent molecular mass 45 kilodaltons and pl values 8.6 to 8.8. was maximally active at pH 4.0 on p-nitrophenyl beta-d-galactoside beta-1,3-linked galactobiose. activity inhibited strongly Hg(2+) 4-chloromercuribenzoate....
The complement system, a part of the innate immune can be activated via three different pathways. In alternative pathway, factor D (FD) plays essential roles in both initiation and amplification loop circulates as an active form. Mannose-binding lectin-associated serine proteases (MASPs) are key enzymes lectin MASP-1 and/or MASP-3 reported to involved activation FD. current study, we generated mice monospecifically deficient for or found that sera MASP-1-deficient lacked pathway activity,...
We recently cloned a novel signaling molecule, p122, that shows GTPase-activating activity specific for Rho and the ability to enhance phosphatidylinositol 4,5-bisphosphate-hydrolyzing of phospholipase C δ1 <i>in vitro</i>. Here we analyzed vivo</i> function p122. Microinjection domain p122 suppressed formation stress fibers focal adhesions induced by lysophosphatidic acid, suggesting as in Transfection also disassembly morphological rounding various adherent cells. Analyses using deletion...
Hydrolysis of inositol phospholipids by receptor stimulation activates two separate signaling pathways, one leading to the activation protein kinase C (C kinase) via formation diacylglycerol. The other is trisphosphate (IP3)/Ca2+ pathway and a major downstream which activated Ca2+/calmodulin-dependent II (CaM II). To examine pathways CaM cytoskeletal vimentin, we prepared monoclonal antibodies YT33 MO82 recognize phosphorylation state vimentin II, respectively. Ectopic expression...
Background — We recently demonstrated that phospholipase C (PLC)–δ1 activity in cultured skin fibroblasts obtained from patients with coronary spastic angina (CSA) is enhanced. tested the hypothesis structural abnormality PLC-δ1 isoform a cause of enhanced activity. Methods and Results Sequence analysis cDNA coding for revealed one conversion guanine to adenine (A) was present at nucleotide position 864 CSA patient, resulting amino acid replacement arginine 257 by histidine (R257H). The...
MBD2, a methyl-CpG-binding protein, is component of the MeCP1 histone deacetylase (HDAC) complex and plays critical role in DNA methylation-mediated transcriptional repression. To understand molecular basis methylation-associated repression, we attempted to identify MBD2-interacting proteins by yeast two-hybrid system. Using MBD2 as bait, isolated novel zinc finger referred MIZF. A direct interaction between MIZF was confirmed vitro binding assays immunoprecipitation experiments. Four seven...
MBD2 is a member of the methyl‐CpG‐binding protein family that plays an important role in methylated DNA silencing. We have recently identified novel zinc finger protein, MIZF, as MBD2‐binding partner. To understand physiological function MIZF MBD2‐mediated gene silencing, we investigated DNA‐binding properties and its potential target genes. Using cyclic amplification selection targets technique, consensus sequence CGGACGTT, which contains conserved CGGAC core, was determined sufficient for...
A GTPase activating protein (GAP), p122, has previously been cloned as a phospholipase C (PLC)δ 1 ‐interacting protein. p122 shows specific GAP activity for Rho and enhances the enzyme of PLCδ . In this study, we examined localization functions p122/RhoGAP, using enhanced green fluorescent (EGFP)‐tagged proteins. EGFP‐p122 was observed punctate structures at plasma membrane BHK (fibroblastic) cells MDCK (epithelial) cells. This patchy distribution depended on cholesterol levels C‐terminal...
The synovial tissue affected by rheumatoid arthritis (RA) is characterized hyperproliferation of cells. High amounts epidermal growth factor (EGF) in the fluid RA patients contribute to To characterize receptor for EGF cells, expression and function ErbB family members were examined.Synovial tissues obtained from surgical excisions. products was examined immunohistochemistry immunoblotting using specific antibodies. Primary cultures established materials. Cell measured MTT. levels...
We have previously reported that Ser13 and Ser34 on glial fibrillary acidic protein (GFAP) in the cleavage furrow of glioma cells are phosphorylated during late mitotic phase (Matsuoka, Y., K. Nishizawa, T. Yano, M. Shibata, S. Ando, Takahashi, Inagaki. 1992, EMBO (Eur. Mol. Biol. Organ.) J. 11:2895-2902). This observation implies a possibility there is kinase specifically activated at metaphase-anaphase transition. To further analyze cell cycle-dependent GFAP phosphorylation, we prepared...
IL-22 is a cytokine that plays pivotal role in regulating tissue homeostasis at barrier surfaces and produced by activated CD4+ Th cells. Currently, the molecular mechanisms Il22 gene expression are still unclear. In this study, we have identified crucial cis-regulatory element located 32 kb upstream of mouse promoter, termed conserved noncoding sequence (CNS)-32. We demonstrated CNS-32 acts as an enhancer reporter assays contains binding motifs for Runt-related transcription factor (Runx)1...
The depletion of stratospheric ozone causes related increase in UV light below about 310 nm, which significantly affects biological and ecological systems. To understand the wavelength-specific effects light, Molt4 cells (human T lymphoma cells) were irradiated with a series monochromatic lights activities three members mitogen-activated protein (MAP) kinase group examined. Extracellular signal-regulated was specifically activated within 1 min after irradiation range 320-360 nm. In contrast,...
A novel HLA-B5 CREG gene, HLA-B SNA was cloned and the primary structure determined. The sequence data showed that identical to HLA-B51 except alpha 1 domain in which one amino acid substitution at residue 74 5 substitutions associated with Bw4/Bw6 epitopes were observed between these Ag. comparison other locus genes suggested evolved from by gene exchange or recombination exon 2 B8. total of 10 14 HLA-B51-specific CTL clones significantly weak no recognition They also gave same degree a...
Hyperplasia of synovial lining cells is one the main features rheumatoid arthritis (RA). We previously reported that ERBB2 highly expressed in RA and it plays an important role their hyperproliferative growth. Recent findings have suggested poly(ADP-ribose) polymerase-1 (PARP-1) involved transactivation NF-κB-dependent genes such as ERBB2. In present study, we investigated PARP-1 transcription cells. The expression level was significantly high derived from three patients with RA, compared...
The complement system plays an important role in host defense and is activated via three different activation pathways. We have previously reported that mannose-binding lectin-associated serine protease (MASP)-3, unlike its splicing variant MASP-1, circulates active form essential for the of alternative pathway (AP) factor D (FD). On other hand, like MASP-1 MASP-2 lectin (LP), MASP-3 forms a complex with pattern recognition molecules (PRMs) LP (LP-PRMs). Both can be efficiently when LP-PRMs...