A5048892363- Marine and coastal plant biology
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Seaweed-derived Bioactive Compounds
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- French Urban and Social Studies
- Ocean Acidification Effects and Responses
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Marine Bivalve and Aquaculture Studies
- Marine Biology and Ecology Research
- Diatoms and Algae Research
- Enzyme Production and Characterization
- Plant Stress Responses and Tolerance
- Metabolomics and Mass Spectrometry Studies
- Marine and coastal ecosystems
- Glutathione Transferases and Polymorphisms
- Protein Hydrolysis and Bioactive Peptides
- Genomics, phytochemicals, and oxidative stress
- Coastal and Marine Management
- Agriculture and Rural Development Research
- Molecular Biology Techniques and Applications
- Marine Sponges and Natural Products
- Water Quality Monitoring and Analysis
Station Biologique de Roscoff
2014-2023
Centre National de la Recherche Scientifique
2013-2023
Sorbonne Université
2013-2023
Laboratoire de Biologie Intégrative des Modèles Marins
2005-2023
Laboratoire des Biomolécules
2004-2016
Université Paris Cité
2012-2015
Centre de Gestion Scientifique
2006-2012
The genome of Ectocarpus, a model organism for brown algae, has been sequenced. Brown algae are complex photosynthetic organisms that have adapted to life in rocky coastal environments. Genome analysis sheds light on this adaptation and reveals an extended set light-harvesting pigment biosynthesis genes novel metabolic processes such as halide metabolism. Comparative genomic analyses highlight the likely importance family receptor kinases related molecules evolution multicellularity plants,...
Red seaweeds are key components of coastal ecosystems and economically important as food a source gelling agents, but their genes genomes have received little attention. Here we report the sequencing 105-Mbp genome florideophyte Chondrus crispus (Irish moss) annotation 9,606 genes. The features an unusual structure characterized by gene-dense regions surrounded repeat-rich dominated transposable elements. Despite its fairly large size, this shows typical compact genomes, e.g., on average...
Brown algae (Phaeophyceae) are phylogenetically distant from red and green an important component of the coastal ecosystem. They have developed unique mechanisms that allow them to inhabit intertidal zone, environment with high levels abiotic stress. Ectocarpus siliculosus is being established as a genetic genomic model for brown algal lineage, but little known about its response Here we examine transcriptomic changes occur during short-term acclimation E. three different stress conditions...
Brown algae are sessile macro-organisms of great ecological relevance in coastal ecosystems. They evolved independently from land plants and other multicellular lineages, therefore hold several original ontogenic metabolic features. Most brown grow along the zone where they face frequent environmental changes, including exposure to toxic levels heavy metals such as copper (Cu). We carried out large-scale transcriptomic metabolomic analyses decipher short-term acclimation algal model E....
Chlorophyll-binding proteins (CBPs) constitute a large family of with diverse functions in both light-harvesting and photoprotection. The evolution CBPs has been debated, especially respect to the origin LI818 subfamily, members which function non-photochemical quenching have found chlorophyll a/c-containing algae several organisms green lineage, but not red so far. recent publication Ectocarpus siliculosus genome represents an opportunity expand on previous work carried out CBPs. codes for...
ABSTRACT The model brown alga Ectocarpus siliculosus undergoes extensive transcriptomic changes in response to abiotic stress, many of them related primary metabolism and particularly amino acid biosynthesis degradation. In this study we seek improve our knowledge the mechanisms underlying stress tolerance alga, particular with regard compatible osmolytes, by examining effects these on metabolite concentrations. We performed metabolic profiling (urea, acids, sugars, polyols, organic fatty...
Brown algae belong to a phylogenetic lineage distantly related green plants and animals, are found predominantly in the intertidal zone, harsh frequently changing environment. Because of their unique evolutionary history habitat, brown feature several peculiarities metabolism. One these is mannitol cycle, which plays central role physiology, as acts carbon storage, osmoprotectant, antioxidant. This polyol derived directly from photoassimilate fructose-6-phosphate via action...
Introduction Saccharina latissima is a canopy-forming species of brown algae and, as such, considered an ecosystem engineer. Several populations this alga are exploited worldwide, and decrease in the abundance S. at its southern distributional range limits has been observed. Despite economic ecological interest, only few data available on composition microbiota associated with role algal physiologyn. Methods We studied whole bacterial community samples from three locations (Brittany,...
To characterize stress and defense‐induced genes in the brown alga Laminaria digitata (Hudson) J.V. Lamouroux, 1985 expressed sequence tags (ESTs) were generated from L. protoplasts. Comparison of ESTs with public databases allowed putative functions to be assigned 45% sequences. sporophytes showed that protoplasts more than intact thalli. Several transcripts gene class coded for proteins involved cell protection against oxygen radicals, including thioredoxins (six ESTs), thioredoxin...
Intertidal seaweeds inhabit an inherently stressful environment with rapidly changing physical conditions the turning tides. Many macroalgae are therefore very resistant to abiotic stress; however, bases for this tolerance and relative importance of different stressors largely unknown. Here, effects stress on transcriptome red seaweed Chondrus crispus were investigated using cDNA microarrays. The responses studied after exposure high light, temperature, hypo- hyperosmotic in laboratory...
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Colonizations of freshwater by marine species are rare events, and little information is known about the underlying mechanisms. Brown algae an independent lineage photosynthetic multicellular organisms from which few inhabit freshwater. As a alga that also found in freshwater, Ectocarpus particular interest for studying transition between these habitats. To gain insights into mechanisms transition, we examined salinity tolerance adaptations to low salinities strain on physiological molecular...
Brown algae are key components of marine ecosystems and live in association with bacteria that essential for their growth development. Ectocarpus siliculosus is a genetic genomic model brown algae. Here we use this to start disentangling the complex interactions may occur between algal host its associated bacteria. We report genome-sequencing 10 alga-associated genome-based reconstruction metabolic networks. The predicted capacities were then used identify complementarities bacteria,...
Abstract Alginate is an industrially important polysaccharide obtained commercially by harvesting brown algae. The final step in alginate biosynthesis, the epimerization of β-1,4-d-mannuronic acid to α-1,4-l-guluronic acid, a structural change that controls physicochemical properties alginate, catalyzed enzyme mannuronan C-5-epimerase. Six different cDNAs with homology bacterial C-5-epimerases were isolated from alga Laminaria digitata (Phaeophyceae). Hydrophobic cluster analysis indicated...
Various abalones and sea-hares from California Brittany (France) were surveyed for their cellulase, fucanase, protease alginate lyase activities. The crude digestive juices contained significant amounts of fucanase but very low levels protease. lyases both the sea hare Aplysia depilans abalone Haliotis tuberculata preferentially degraded polymannuronate sequences. partially purified by cation exchange chromatography. For purpose protoplast preparation brown algae, purification procedure was...
Journal Article Isolation Conditions for High Yields of Protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae) Get access DECLAN M. BUTLER, BUTLER 1Department Pure Applied Biology, The University LeedsLeeds LS2 9JT, UK3Centre d'Etudes Océanologiques et de Biologie MarineCNRS, UPR 4601, 29211 Roscoff, France Search other works by this author on: Oxford Academic PubMed Google Scholar KJETILL ØSTGAARD, ØSTGAARD 2Laboratory Biotechnology, Norwegian Institute TechnologyTrondheim,...
Abstract Background The production of stable and soluble proteins is one the most important steps prior to structural functional studies biological importance. We investigated parallel in a medium throughput strategy genes coding for from various marine organisms, using protocols that involved recombinatorial cloning, protein expression screening batch purification. This was applied order respond need post-genomic validation recent success large number genomic projects. Indeed, upcoming...
Summary Brown algae (stramenopiles) are key players in intertidal ecosystems, and represent a source of biomass with several industrial applications. Ectocarpus siliculosus is model to study the biology these organisms. Its genome has been sequenced number post‐genomic tools have implemented. Based on this knowledge, we report reconstruction analysis genome‐scale metabolic network for E. , Ecto GEM ( http://ectogem.irisa.fr ). This atlas pathways consists 1866 reactions 2020 metabolites, its...
Rhizobiales and related orders of Alphaproteobacteria comprise several genera nodule-inducing symbiotic bacteria associated with plant roots. Here we describe the genome metabolic network "Candidatus Phaeomarinobacter ectocarpi" Ec32, a member new candidate genus closely to found in association cultures filamentous brown algal model Ectocarpus. The "Ca. P. encodes numerous pathways that may be relevant for this bacterium interact algae. Notably, it possesses large set glycoside hydrolases...
Coastal areas form the major habitat of brown macroalgae, photosynthetic multicellular eukaryotes that have great ecological value and industrial potential. Macroalgal growth, development, physiology are influenced by microbial community they accommodate. Studying algal microbiome should thus increase our fundamental understanding biology may help to improve culturing efforts. Currently, a freshwater strain macroalga Ectocarpus subulatus is being developed as model organism for macroalgal...