A5087525010- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Photosynthetic Processes and Mechanisms
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- Retinoids in leukemia and cellular processes
- Plant Stress Responses and Tolerance
- Protein Degradation and Inhibitors
- Multiple Myeloma Research and Treatments
- Genomic variations and chromosomal abnormalities
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Hematopoietic Stem Cell Transplantation
- Viral-associated cancers and disorders
- T-cell and Retrovirus Studies
- Algal biology and biofuel production
- Chromosomal and Genetic Variations
- Trace Elements in Health
- CRISPR and Genetic Engineering
Munich Leukemia Laboratory (Germany)
2016-2025
Ludwig-Maximilians-Universität München
2007-2012
Urologische Klinik München
2010
Center for Integrated Protein Science Munich
2008-2010
Institute of Virology of the Slovak Academy of Sciences
2006
Institute of Groundwater Ecology
2005-2006
Technical University of Munich
2005-2006
Lineage-ambiguous leukemias are high-risk malignancies of poorly understood genetic basis. Here, we describe a distinct subgroup acute leukemia with expression myeloid, T lymphoid, and stem cell markers driven by aberrant allele-specific deregulation BCL11B, master transcription factor responsible for thymic T-lineage commitment specification. Mechanistically, this was chromosomal rearrangements that juxtapose BCL11B to superenhancers active in hematopoietic progenitors, or focal...
Summary Detoxification of xenobiotic compounds and heavy metals is a pivotal capacity organisms, in which glutathione (GSH) plays an important role. In plants, electrophilic herbicides are conjugated to the thiol group GSH, metal ions form complexes as thiolates with GSH‐derived phytochelatins (PCs). both detoxification processes phytochelatin synthase (PCS) emerges key player. The enzyme activated by catalyzes PC formation from GSH transferring glutamylcysteinyl residues ( γ ‐EC) onto GSH....
Abstract Translocation of nuclear-encoded preproteins across the inner envelope chloroplasts is catalyzed by Tic translocon, consisting Tic110, Tic40, Tic62, Tic55, Tic32, Tic20, and Tic22. Tic62 was proposed to act as a redox sensor complex because its redox-dependent shuttling between stroma specific interaction with photosynthetic protein ferredoxin-NADP(H) oxidoreductase (FNR). However, nature this close relationship so far remained enigmatic. A putative additional localization at...
In the cyanobacterium Synechocystis sp PCC 6803, early steps in thylakoid membrane (TM) biogenesis are considered to take place specialized fractions resembling an interface between plasma (PM) and TM. This region (the PratA-defined membrane) is defined by presence of photosystem II (PSII) assembly factor PratA (for processing-associated TPR protein) precursor D1 protein (pD1). Here, we show that a Mn2+ binding contains high affinity site (K d = 73 μM) required for efficient delivery PSII...
T-cell prolymphocytic leukemia (T-PLL) is a rare post-thymic neoplasm with aggressive clinical course and short overall survival. So far, due to the rareness of this disease, genetic data are available only from individual cases or small cohorts. In our study, we aimed at performing comprehensive cytogenetic molecular characterization T-PLL comprising largest cohort patients analyzed so including correlations between respective markers their impact on prognosis. Genetic abnormalities were...
Acquired somatic mutations are crucial for the development of most cancers. We performed a comprehensive comparative analysis mutational landscapes and their correlation with CHIP-related (clonal hematopoiesis indeterminate potential) patient age 122 genes in 3096 cases 28 different hematological malignancies. Differences were observed regarding (1) median number (highest, n = 4; lowest, 0); (2) specificity certain (high frequencies atypical chronic myeloid leukemia [aCML; ASXL1, 86%],...
The preprotein translocon at the inner envelope of chloroplasts (Tic complex) facilitates import nuclear-encoded preproteins into organelle. Seven distinct subunits have been identified so far. For each those, specific functions proposed based on structural prediction or experimental evidence. Three those possess modules that could act as redox-active regulatory components in process. To date, however, mode redox regulation process remains enigmatic. investigate how chloroplast state...
Fusion transcripts are frequent genetic abnormalities in myeloid malignancies and often the basis for risk stratification, minimal residual disease (MRD) monitoring, targeted therapy. We comprehensively analyzed fusion transcript landscape 572 acute leukemia (AML) 630 myelodysplastic syndrome (MDS) patients by whole transcriptome sequencing (WTS). Totally, 274 events (131 unique fusions) were identified 210/572 AML (37%). In 16/630 MDS patients, 16 (15 detected (3%). AML, 141 cases comprised...
Several clinical and genetic factors impact overall survival (OS) in myelodysplastic neoplasms (MDS) acute myeloid leukemia (AML), including complex karyotype (CK), TP53 allelic state, blast count. We analyzed the interplay of these by performing Cox regression analysis determining frequency single-hit (sh) double-hit (dh) events OS MDS (n = 747) with <5% blasts, ≥5% but <10% ≥10% <20% blasts AML 772). showed best outcome, followed (median OS: 75, 54, 27, 18 months, respectively). The same...
TP53 is the most frequently mutated gene in cancer also playing important roles pathogenesis of hematologic malignancies. 1,2][10][11] Several targeted drugs and novel therapeutic options have been developed recent years; however, optimal treatment strategy for patients with AML MDS harboring TP53alt remains a critical area unmet need.TP53alts comprise not only mutations but allelic imbalances, including deletions (dels) regions copy-neutral loss heterozygosity (cnLOH) comprising...
Ferredoxin:NADPH oxidoreductase (FNR) is a key enzyme of photosynthetic electron transport required for generation reduction equivalents. Recently, two proteins were found to be involved in membrane-anchoring FNR by specific interaction via conserved Ser/Pro-rich motif: Tic62 and Trol. Our crystallographic study reveals that the FNR-binding motif, which forms polyproline type II helix, induces self-assembly monomers into back-to-back dimer. Because binding occurs opposite active sites, its...
Early steps in the biogenesis of Photosystem II (PSII) cyanobacterium Synechocystis sp. PCC 6803 are thought to occur a specialized membrane fraction that is characterized by specific accumulation PSII assembly factor PratA and its interaction partner pD1, precursor D1 protein PSII. Here, we report molecular characterization this fraction, called PratA-defined (PDM), with regard lipid pigment composition association factors, including YCF48, Slr1471, Sll0933, Pitt. We demonstrate YCF48...
The function and structure of protein translocons at the outer inner envelope membrane chloroplasts (Toc Tic complexes, respectively) are a subject intensive research. One proteins that have been ascribed to complex is Tic62. This was proposed as redox sensor may possibly act regulator during translocation process. Tic62 bimodular comprises an N-terminal module, responsible for binding pyridine nucleotides, C-terminal module which serves docking site ferredoxin-NAD(P)-oxido-reductase (FNR)....
Considering the clinical and genetic characteristics, acute lymphoblastic leukemia (ALL) is a rather heterogeneous hematological neoplasm for which current standard diagnostics require various analyses encompassing morphology, immunophenotyping, cytogenetics, molecular analysis of gene fusions mutations. Hence, it would be desirable to rely on technique an analytical workflow that allows simultaneous identification all alterations in single approach. Moreover, based results with methods,...