A5085293018- Cancer Immunotherapy and Biomarkers
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- HIV-related health complications and treatments
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Gut microbiota and health
- Cancer Research and Treatments
- Immune Cell Function and Interaction
- Tryptophan and brain disorders
- Clostridium difficile and Clostridium perfringens research
- Phagocytosis and Immune Regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- HIV Research and Treatment
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Nanoplatforms for cancer theranostics
- Cancer-related gene regulation
- Angiogenesis and VEGF in Cancer
- Complement system in diseases
- Bladder and Urothelial Cancer Treatments
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
Carisma Therapeutics (United States)
2020-2025
University of Pennsylvania
2015-2024
Cancer Research Center
2015-2023
Hospital of the University of Pennsylvania
2023
Ovarian Cancer Research Alliance
2018-2021
California University of Pennsylvania
2021
Philadelphia University
2017
Medical University of Sofia
2012-2015
Università di Camerino
2015
Utrecht University
2012
Alterations in gut microbiota impact the pathophysiology of several diseases, including cancer. Radiotherapy (RT), an established curative and palliative cancer treatment, exerts potent immune modulatory effects, inducing tumor-associated antigen (TAA) cross-priming with antitumor CD8+ T cell elicitation abscopal effects. We tested whether modulates response following RT distal to gut. Vancomycin, antibiotic that acts mainly on gram-positive bacteria is restricted gut, potentiated RT-induced...
Adoptive T cell therapy (ACT) is a promising new modality for malignancies. Here, we report that adoptive efficacy in tumor-bearing mice significantly affected by differences the native composition of gut microbiome or treatment with antibiotics, heterologous fecal transfer. Depletion bacteria vancomycin decreased rate tumor growth from The Jackson Laboratory receiving ACT, whereas neomycin and metronidazole had no effect, indicating role specific host response. Vancomycin induced an...
Several studies have shown the influence of commensal microbes on T cell function, specifically in setting checkpoint immunotherapy for cancer. In this study, we investigated how vancomycin-induced gut microbiota dysbiosis affects chimeric antigen receptor (CAR) using multiple preclinical models as well clinical correlates. two murine tumor models, hematopoietic CD19+-A20 lymphoma and CD19+-B16 melanoma, mice receiving vancomycin combination with CD19-directed CAR (CART-19) therapy displayed...
Cancer immunotherapy relies upon the ability of T cells to infiltrate tumors. The endothelium constitutes a barrier between tumor and effector cells, manipulate local vascular permeability could be translated into effective immunotherapy. Here, we show that in context adoptive cell therapy, antitumor delivered at high enough doses, can overcome endothelial tumors, process requires production C3, complement activation on release C5a. C5a, turn, acts promoting upregulation adhesion molecules...
Laryngeal squamous cell carcinoma (laryngeal SCC) is a frequently occurring cancer of the head and neck area. Epigenetic changes tumor-related genes contribute to its genesis progression.We assessed promoter methylation status selected (CDKN2A, MGMT, MLH1, DAPK) using methylation-sensitive high resolution melting (MS-HRM) in 100 patients with laryngeal SCC studied correlations clinical characteristics.The prevalence CDKN2A, DAPK was 59 97 (60.8%), 46 (47.4%), 45 (46.4%), 41 (42.3%),...
Background Tumor endothelial marker 1 (TEM1) is a protein expressed in the tumor-associated endothelium and/or stroma of various types cancer. We previously demonstrated that immunization with plasmid-DNA vaccine targeting TEM1 reduced tumor progression three murine cancer models. Radiation therapy (RT) an established modality used more than 50% patients solid tumors. RT can induce vasculature injury, triggering immunogenic cell death and inhibition irradiated distant non-irradiated growth...
Mitochondria provide energy for cells via oxidative phosphorylation. Reactive oxygen species, a byproduct of this mitochondrial respiration, can damage DNA (mtDNA), and somatic mtDNA mutations have been found in all colorectal, ovarian, breast, urinary bladder, kidney, lung, pancreatic tumors studied. The resulting altered proteins or tumor-associated Ags (TAMAs) are potentially immunogenic, suggesting that they may be targetable cancer immunotherapy. In article, we show the RENCA tumor cell...
Granulosa cell tumors (GCT) are rare ovarian malignancies. Due to the lack of effective treatment in late relapse, there is a clear unmet need for novel therapies. Forkhead Box L2 (FOXL2) protein mainly expressed granulosa cells (GC) and therefore rational therapeutic target. Since we identified tumor infiltrating lymphocytes (TILs) as main immune population within GCT, TILs from 11 GCT patients were expanded, their phenotypes interrogated determine that T acquired antigen-experienced lower...
Abstract Despite the remarkable efficacy achieved by CAR-T cell therapy in hematologic malignancies, translating these results solid tumors remains challenging. We previously developed human CAR-M and demonstrated that adoptive transfer of into xenograft models cancer controls tumor progression improves overall survival1. Herein, we established a fully immunocompetent syngeneic mouse model evaluated interaction with microenvironment (TME) endogenous adaptive immune system. Murine bone...
Abstract Parkin is an E3 ubiquitin ligase, which plays a key role in the development of Parkinson disease. defects also occur numerous cancers, and growing body evidence indicates that functions as tumor suppressor impedes number cellular processes involved tumorigenesis. Here, we generated murine human models closely mimic advanced-stage tumors where deficiencies are found to provide deeper insights into suppressive Parkin. Loss expression led aggressive growth, was associated with poor...
<h3>Background</h3> Despite the remarkable efficacy achieved by CAR-T therapy in hematologic malignancies, application solid tumors has been challenging. We previously developed human CAR-M and demonstrated that adoptive cell transfer of into xenograft models cancer controls tumor progression improves overall survival [1]. Given are professional antigen presenting cells, we an immunocompetent animal model to evaluate potential for induction a systemic anti-tumor immune response....
<h3>Background</h3> Despite the remarkable efficacy achieved by CAR-T therapy in hematologic malignancies, application solid tumors has been challenging. We previously developed human CAR-M and demonstrated that adoptive transfer of into xenograft models cancer controls tumor progression improves overall survival.<sup>1</sup> Given are M1-polarized macrophages with potential to remodel microenvironment (TME) act as professional antigen presenting cells, we an immunocompetent animal model...
Abstract The sheer enormity of the microbial biomass in human intestinal tract, co-evolution between humans and microbiota, established function gut microbes regulating normal host physiologic functions, are all consistent with idea that alterations ecology play a role pathophysiology several conditions including cancer. Radiotherapy (RT) is an curative palliative cancer treatment regimen, approximately half patients solid tumors receiving RT some time during their disease. Mounting evidence...
Abstract In recent years, research has highlighted radiotherapy's (RT) capacity to induce Immunogenic Cell Death (ICD), activating tumor-infiltrating Dendritic Cells (DCs) for cross-presenting tumor-associated antigens (TAAs), leading robust systemic immune responses. Simultaneously, emerging studies underscore the gut microbiota's crucial role in modulating immunity across diverse human diseases. Our investigation focuses on microbiome modulation, specifically using oral Vancomycin, a...
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<div>Abstract<p>Parkin is an E3 ubiquitin ligase that plays a key role in the development of Parkinson’s disease. Parkin defects also occur numerous cancers, and growing body evidence indicates functions as tumor suppressor impedes number cellular processes involved tumorigenesis. Here, we generated murine human models closely mimic advanced-stage tumors where deficiencies are found to provide deeper insights into suppressive Parkin. Loss expression led aggressive growth was...