A5009307368- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- Wastewater Treatment and Nitrogen Removal
- Pesticide and Herbicide Environmental Studies
- Toxic Organic Pollutants Impact
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Constructed Wetlands for Wastewater Treatment
- Microbial Metabolic Engineering and Bioproduction
- Advanced Chemical Sensor Technologies
- Coastal wetland ecosystem dynamics
- Air Quality Monitoring and Forecasting
- Electrokinetic Soil Remediation Techniques
- Atmospheric chemistry and aerosols
- Bacteriophages and microbial interactions
- Mechanical Failure Analysis and Simulation
- Meat and Animal Product Quality
- Water Treatment and Disinfection
- Microplastics and Plastic Pollution
- Enzyme-mediated dye degradation
- Gut microbiota and health
- Food Safety and Hygiene
Guangzhou Institute of Geochemistry
2017-2025
Chinese Academy of Sciences
2017-2025
University of Chinese Academy of Sciences
2017-2025
Shenyang University of Technology
2025
Chengdu University of Traditional Chinese Medicine
2022
Hohai University
2020
Guangdong Academy of Sciences
2014-2015
Shanghai Ocean University
2013-2014
Illinois Institute of Technology
2013
Southwest Jiaotong University
2007
The indigenous microorganisms responsible for degrading phenanthrene (PHE) in polycyclic aromatic hydrocarbons (PAHs)-contaminated wastewater were identified by DNA-based stable isotope probing (DNA-SIP). In addition to the well-known PHE degraders Acinetobacter and Sphingobium, Kouleothrix Sandaracinobacter found, first time, be directly biodegradation. Additionally, a novel degrader, tandoii sp. LJ-5, was DNA-SIP direct cultivation. This is report reference A. involved bioremediation of...
To reveal the mechanisms of autochthonous bioaugmentation (ABA) in wastewater contaminated with polycyclic aromatic hydrocarbons (PAHs), DNA-stable-isotope-probing (SIP) was used present study addition an microorganism Acinetobacter tandoii LJ-5. We found LJ-5 inoculum produced a significant increase phenanthrene (PHE) mineralization, but surprisingly did not participate indigenous PHE degradation from SIP results. The improvement biodegradation explained by engagement attributed to...
Magnetic-nanoparticle-mediated isolation coupled with stable-isotope probing (MMI–SIP) is a cultivation-independent higher-resolution approach for isolating active degraders in their natural habitats. However, it addresses the community level and cannot directly link microbial identities, phenotypes, situ functions of at single-cell within complex communities. Here, we used 13C-labeled phenanthrene as target developed new method coupling MMI–SIP Raman-activated cell sorting (RACS), namely,...
Polycyclic aromatic hydrocarbon (PAH) derivatives, including alkyl-PAHs, are significant emerging environmental contaminants. Traditional culture techniques often fail to isolate functional microorganisms responsible for their in situ degradation, leaving metabolic interactions and pathways largely uncharacterized. This study employs 2-methylnaphthalene (MP) as a model compound utilizes Raman-activated cell sorting with stable isotope probing (RACS-SIP) single-cell genome sequencing...
The identification and in situ cultivation of functional yet uncultivable microorganisms are important to confirm inferences regarding their ecological functions. Here, we developed a new method that couples Raman-activated cell sorting (RACS), stable-isotope probing (SIP), genome-directed (GDC)─namely, RACS-SIP-GDC─to identify, sort, cultivate the active toluene degraders from complex microbial community petroleum-contaminated soil. Using SIP, successfully identified degrader Pigmentiphaga,...
Abstract Soil microbial flora constitutes a highly diverse and complex microbiome on Earth, often challenging to cultivation, with unclear metabolic mechanisms in situ. Here, we present pioneering concept for the situ construction of functional consortia (FMCs) introduce an innovative method creating FMCs by utilizing phenanthrene as model compound elucidate their biodegradation mechanisms. Our methodology involves single-cell identification, sorting, culture microorganisms, resulting...
Electrolyzed water ice is a relatively new concept developed in food industry recent years. The effect of acidic electrolyzed (AEW) on preserving the quality shrimp (Litopenaeus vannamei) was investigated. Physical, chemical, and microbiological changes were examined during storage. results showed that compared with tap (TW) ice, AEW displayed potential ability limiting pH flesh significantly (p < 0.05) retarded color difference formation total volatile basic nitrogen (TVBN). And treatment...
Autochthonous bioaugmentation (ABA) strategies are primarily carried out using a single, highly efficient type of bacteria that is capable directly degrading the target compound. However, no studies have examined use non-direct degraders (NDDs), which involved in metabolic pathway compounds instead direct degradation. Here, to evaluate bioremediation efficiency and mechanism ABA by NDDs, we demonstrated an NDD on biodegradation biphenyl, model compound used study polychlorinated biphenyl...
Rhizoremediation is a promising remediation technology for the removal of soil persistent organic pollutants (POPs), especially benzo[a]pyrene (BaP). However, our understanding associations among rhizospheric metabolites, functional microorganisms, and POPs degradation in different plant growth stages limited. We combined stable-isotope probing (SIP), high-throughput sequencing, metabolomics to analyze changes microbes, BaP biodegradation early (tillering, jointing) later stage (booting)...
To identify and obtain the indigenous degraders metabolizing phenanthrene (PHE) biphenyl (BP) from complex microbial community within industrial wastewater, DNA-based stable-isotope probing (DNA-SIP) cultivation-based methods were applied in present study. DNA-SIP results showed that two bacterial taxa (Vogesella Alicyclobacillus) considered key biodegraders responsible for PHE biodegradation only, whereas Bacillus Cupriavidus involved BP degradation. Vogesella Alicyclobacillus have not been...