A5102801749- Aortic aneurysm repair treatments
- Diagnosis and Treatment of Venous Diseases
- Peripheral Artery Disease Management
- Venous Thromboembolism Diagnosis and Management
- Aortic Disease and Treatment Approaches
- Infectious Aortic and Vascular Conditions
- Central Venous Catheters and Hemodialysis
- Protein Kinase Regulation and GTPase Signaling
- Molecular Biology Techniques and Applications
- Cardiac, Anesthesia and Surgical Outcomes
- Coronary Interventions and Diagnostics
- Cell Adhesion Molecules Research
- Cerebrovascular and Carotid Artery Diseases
- Wound Healing and Treatments
- Infective Endocarditis Diagnosis and Management
- Cancer-related Molecular Pathways
- Orthopedic Infections and Treatments
- Cardiac Fibrosis and Remodeling
- Reconstructive Surgery and Microvascular Techniques
- Vascular Procedures and Complications
- Electrospun Nanofibers in Biomedical Applications
- Cardiovascular Health and Disease Prevention
- RNA Interference and Gene Delivery
- FOXO transcription factor regulation
- MicroRNA in disease regulation
University of Maryland, Baltimore
2010-2020
University of Maryland Medical Center
2011-2020
Veterans Health Administration
2015-2019
VA Maryland Health Care System
2019
Baltimore VA Medical Center
2016
Beth Israel Deaconess Medical Center
2003-2010
Harvard University
2007-2010
University of California, San Francisco
2009-2010
Center for Vascular Biology Research
2010
Beth Israel Deaconess Hospital
2009
Background Accelerated atherosclerosis is the leading cause of morbidity and mortality in diabetic patients. Hyperglycemia a recognized independent risk factor for heightened atherogenesis diabetes mellitus (DM). However, our understanding mechanisms underlying glucose damage to vasculature remains incomplete. Methodology/Principal Findings High hyperglycemia reduced upregulation NF-κB inhibitory atheroprotective protein A20 human coronary endothelial (EC) smooth muscle cell (SMC) cultures...
Adenosine A(2a)-receptor activation enhances shortening of isolated cardiomyocytes. In the present study effect on contractile performance rat hearts was investigated by recording left ventricular pressure (LVP) and maximal rate LVP development (+dP/dt(max)). With constant-pressure perfusion, adenosine caused concentration-dependent increases in +dP/dt(max), with detectable 4.1 4.8% at 10(-6) M 12.0 11.1% 10(-4) M, respectively. The responses were prevented antagonists chlorostyryl-caffeine...
Transcription of the myristoylated alanine-rich C kinase substrate (MARCKS) is upregulated in animal models intimal hyperplasia. MARCKS knockdown inhibits vascular smooth muscle cell (VSMC) migration vitro; however, mechanism as yet unknown. We sought to elucidate MARCKS-mediated motility and determine whether reduces hyperplasia formation vivo.MARCKS blocked platelet-derived growth factor (PDGF)-induced translocation cortactin cortex, impaired both lamellipodia filopodia formation,...
ObjectiveChronic venous leg ulcers (VLUs) affect up to 2% of the general population, resulting in a significant socioeconomic burden. Placental tissue that contains mesenchymal stem cells and active growth factors has been shown be beneficial healing chronic wounds. We compared efficacy human viable wound matrix (hVWM) cryopreserved placental for treatment refractory VLUs with standard therapy.MethodsThis prospective single-center open-label single-arm study enrolled patients Clinical,...
Intimal hyperplasia (IH) limits the patency of all cardiovascular vein bypass grafts. We previously found myristoylated alanine-rich C kinase substrate (MARCKS), a key protein (PKC) substrate, to be up-regulated in canine models IH. Here, we further characterize role MARCKS IH and examine phenotypic consequences silencing by small interfering RNA (siRNA) transfection human vascular smooth muscle cells (VSMCs) endothelial (ECs) vitro use rapid 10-min nonviral siRNA technique determine effects...
Clinical sequelae from blunt cardiac trauma (BCT) may range minor electrocardiographic abnormalities to death free-wall rupture. There are no established clinical characteristics or injury scoring systems that able predict survival in these patients.A retrospective review of medical records a Level I center identified 47 patients with BCT. A grade assigned on the basis American Association for Surgery Trauma Organ Injury Scale (OIS) was each case studied. data, including Severity Score...
Medical student surgical training has traditionally occurred in the operating room (OR). Present technology allows real time communication between OR and a remote classroom. We seek to evaluate value of teleconferencing (TC) environment compared with traditional environment. Students enrolled third-year core clerkship surgery participated both TC teaching sessions. A total 23 sessions were conducted observed (TC=8; OR=15). In sessions, students asked over 4 times as many questions (17.4±8.5...
Background Overexpression of the myristolated alanine-rich C kinase substrate (MARCKS) occurs in vascular proliferative diseases such as restenosis after bypass surgery. MARCKS knockdown results arrest smooth muscle cell (VSMC) proliferation with little effect on endothelial (EC) proliferation. We sought to identify mechanism differential regulation by VSMC and EC vitro vivo. Methods Results siRNA-mediated VSMCs inhibited prevented progression from phase G0/G1 S. Protein expression...
Objectives: To report the results of a novel approach using supine positioning for radiofrequency ablation (RFA) small saphenous vein (SSV) with combined great (GSV). Methods: Over 24-month period, we identified patients symptomatic SSV incompetence. Access to was accomplished by ultrasound-guided venipuncture patient in position. Results: Small performed on 27 limbs 26 patients. Median follow-up 94 days (interquartile range [IQR] 26, 171). Mean clinical–etiologic–anatomic–pathophysiologic...
Trauma, whether caused by an accident or in intentional manner, results significant morbidity and mortality. The goal of this study was to develop a novel biomaterial surface vitro ex vivo that provides both localized infection resistance nd hemostatic properties. Our hypothesis is combination specific characteristics can be successfully incorporated into single biomaterial. Functional groups were created with woven Dacron (Cntrl) material via exposure ethylenediamine (C-EDA). antibiotic...
Selective CD28 inhibition is actively pursued as an alternative to B7 blockade using cytotoxic T lymphocyte antigen 4 Ig based on the hypothesis that checkpoint immune regulators and programmed death ligand 1 will induce tolerogenic signals. We previously showed blocking a monovalent nonactivating reagent (single-chain anti-CD28 Fv fragment linked alpha-1 antitrypsin [sc28AT]) synergizes with calcineurin inhibitors in nonhuman primate (NHP) kidney heart transplantation. Here, we explored...