A5056655281- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- CRISPR and Genetic Engineering
- Advanced Vision and Imaging
- Neural dynamics and brain function
- Electrohydrodynamics and Fluid Dynamics
- Electrowetting and Microfluidic Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Nanomaterials and Printing Technologies
- Cognitive Science and Mapping
- Zebrafish Biomedical Research Applications
- Scientific Computing and Data Management
- Human Pose and Action Recognition
Cold Spring Harbor Laboratory
2021-2024
University of Oxford
2018-2021
Significance Despite improvements in our ability to manipulate ever-smaller volumes, most workflows cell biology still use volumes of many microliters. We describe a method for creating microfluidic arrangements containing submicroliter volumes. It exploits interfacial forces dominant at the microscale confine liquids with fluid (not solid) walls. demonstrate basic manipulations required culture and some widely used downstream workflows. The eliminates problems associated fabrication...
Microfluidics with fluid walls opens up the realm of reconfiguring cellular environments during assays.
There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and immiscible fluorocarbon (FC40). A submerged microjet FC40 projected through media onto the bottom dish, where it washes away to leave liquid walls pinned substrate by interfacial forces. Such fluid can be built into almost any imaginable 2D circuit...
Single-cell isolation and cloning are essential steps in many applications, ranging from the production of biotherapeutics to stem cell therapy. Having confidence monoclonality such applications is both research commercial perspectives, for example, ensure that data high quality regulatory requirements met. Consequently, several approaches have been developed improve monoclonality. However, ensuring using standard well plate formats remains challenging, primarily due edge effects; solid wall...
Abstract The aqueous phase in traditional microfluidics is usually confined by solid walls; flows through such systems are often predicted accurately. As walls limit access, open being developed which the partly bounded fluid (interfaces with air or immiscible liquids). Such morph during flow due to pressure gradients, so predicting fields remains challenging. We recently a version of suitable for live-cell biology an interface and bioinert fluorocarbon (FC40). Here, we find that common...
Many wound-healing assays are used in cell biology and biomedicine; they often labor intensive and/or require specialized costly equipment. We describe a contactless method to create wounds with any imaginable 2D pattern monolayers using the micro-jets of either media or an immiscible biocompatible fluorocarbon (i.e., FC40). also combine this another that allows automation multiplexing standard Petri dishes. A dish is filled thin film overlaid FC40, two liquids reshaped into array...
ABSTRACT There is an unmet demand for microfluidics in biomedicine. We describe contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, 3-way traverse, cell-culture media, and immiscible fluorocarbon (FC40). A submerged micro-jet FC40 projected through media to the bottom dish, where it washes away leave liquid walls pinned substrate by interfacial forces. Such fluid can be built into almost any imaginable 2D circuit minutes,...
Abstract Many wound-healing assays are used in cell biology and biomedicine; they often labor intensive and/or require specialized costly equipment. We describe a contactless method to create wounds with any imaginable 2D pattern monolayers using micro-jets of either media or an immiscible biocompatible fluorocarbon (i.e., FC40). also combine this another that allows automation multiplexing standard Petri dishes. A dish is filled thin film overlaid FC40, the two liquids reshaped into array...