A5055701414- Genetic Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Hippo pathway signaling and YAP/TAZ
- Body Contouring and Surgery
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
- Fungal and yeast genetics research
- Cancer-related gene regulation
- Sirtuins and Resveratrol in Medicine
- Mesenchymal stem cell research
- Diabetic Foot Ulcer Assessment and Management
- Protein Structure and Dynamics
- Cellular Mechanics and Interactions
- Heat shock proteins research
- Dermatologic Treatments and Research
- Biochemical and Structural Characterization
- Histone Deacetylase Inhibitors Research
- Microbial Metabolic Engineering and Bioproduction
University of California, Irvine
1994-2023
University of California, Los Angeles
1995-1998
ABSTRACT The dishevelled gene of Drosophila is required to establish coherent arrays polarized cells and also segments in the embryo. Here, we show that loss function clones, double heterozygotes with wingless mutants flies bearing a weak transgene leads patterning defects which phenocopy observed alone. Further, all body require planar cell polarity, some alleles dishevelled; exhibit bristle polarity identical those seen requirement for establishing autonomous. encodes novel intracellular...
Protein acetylation, which is central to transcriptional control as well other cellular processes, disrupted in Huntington's disease (HD). Treatments that restore global acetylation levels, such inhibiting histone deacetylases (HDACs), are effective suppressing HD pathology model organisms. However, agents selectively target the disease-relevant HDACs have not been available. SirT1 (Sir2 Drosophila melanogaster) deacetylates histones and proteins including transcription factors. Genetically...
Wnt regulation of gene expression requires binding LEF/T-cell factor (LEF/TCF) transcription factors toWnt response elements (WREs) and recruitment the activator -catenin.There are significant differences in abilities LEF/TCF family members to regulate target genes.For example, alternatively spliced isoforms TCF-1 TCF-4 with a C-terminal "E" tail uniquely potent their activation LEF1 CDX1.Here we report that mechanism responsible for this unique activity is an auxiliary 30-amino-acid DNA...
Abstract Huntington disease (HD) is an autosomal neurodegenerative disorder caused by the expansion of Polyglutamine (polyQ) in exon 1 Huntingtin protein. Glutamine repeats below 36 are considered normal while above 40 lead to HD. Impairment energy metabolism a common trend pathogenesis; however, this effect not fully understood. Here, we used phasor approach and Fluorescence Lifetime Imaging Microscopy (FLIM) measure changes between free bound fractions NADH as indirect metabolic alteration...
The protein σ 54 associates with Escherichia coli core RNA polymerase to form a holoenzyme that binds promoters but is inactive in the absence of enhancer activation. Here, mutants enabled polymerases transcribe without and adenosine triphosphate. mutations are leucines within NH 2 -terminal glutamine-rich domain . Multiple leucine substitutions mimicked effect protein, which suggests functions disrupt patch. results indicate acts both as an inhibitor activity receptor interacts overcome...
Sigma 54 is a required factor for bacterial RNA polymerase to respond enhancers and directs mechanism that hybrid between eukaryotic transcription. Three pathways were found bypass the enhancer requirement in vitro . These rely on either deletion of sigma N terminus or destruction DNA consensus −12 promoter recognition element altering solution conditions favor transient melting. Each these allows unstable heparin-sensitive pre-initiation complexes form can be driven transcribe absence both...
Background Spatially restricted morphogen expression drives many patterning and regeneration processes, but how is the pattern of established maintained? Patterning Drosophila leg imaginal discs requires DPP dorsally wingless (WG) ventrally. We have shown that these mutually exclusive patterns are controlled by a self-organizing system feedback loops involve WG DPP, whether direct or indirect not known. Methods/Findings By analyzing regulatory DNA driving reporter genes in different genetic...
Huntington's disease (HD) is caused by an expansion of a poly glutamine (polyQ) stretch in the huntingtin protein (HTT) that necessary to cause pathology and formation HTT aggregates. Here we ask whether expanded polyQ sufficient aggregate formation. By addressing sufficiency question, one can identify cellular processes structural parameters influence HD subcellular behavior (i.e. aggregation state location). Using Drosophila, compare effects expressing mutant full-length human (fl-mHTT)...
ABSTRACT Sigma 54 associates with bacterial core RNA polymerase and converts it into an enhancer-responsive enzyme. Deletion of the N-terminal 40 amino acids is known to result in loss ability respond enhancer binding proteins. In this work PCR mutagenesis genetic screens were used identify a small patch, from 33 37, that required for proper response activator vivo. Site-directed single point mutants within segment constructed studied. Two these defective responding protein vitro. The could...
The N‐terminal activation domain of Escherichia coli sigma 54 was randomly mutated to provide a library changes that might allow the required enhancer function be bypassed. Five clones harbouring mutant factors were obtained exhibited this property in they enhanced growth under nitrogen‐limiting conditions cells lacking NtrC. DNA sequence analysis located all mutations four leucines small region between amino acids 25 and 31. No retained hydrophobic character leucine residues. Mutant shown...
Background: Current minimally invasive fat reduction modalities utilize equipment that can cost thousands of United States dollars. Electrochemical Lipolysis (ECLL), utilizing low-cost battery and electrodes (about $10), creates acid/base within (width ~3 mm), damaging adipocytes. Longitudinal effects ECLL have not been studied. In this pilot study, we hypothesize in vivo induces necrosis, decreases adipocyte number/viability, forms lipid droplets (LDs). Methods: Two female Yorkshire pigs...
Abstract Objectives Minimally invasive fat sculpting techniques are becoming more widespread with the development of office‐based devices and therapies. Electrochemical lipolysis (ECLL) is a needle‐based technology that uses direct current (DC) to electrolyze tissue water creating acid base in situ. In turn, saponified adipocyte cell membrane lysis occurs. The electrolysis can be accomplished using simple open‐loop circuit (V‐ECLL) or by incorporating feedback control potentiostat (P‐ECLL)....
In this paper, we consider a mathematical model for the forma- tion of spatial morphogen territories two key morphogens: Wingless (Wg) and Decapentaplegic (DPP), involved in leg development Drosophila. We define gene regulatory network (GRN) that utilizes autoactivation cross- inhibition (modeled by Hill equations) to establish maintain stable bound- aries expression. By computational analysis find presence general activator, neither autoactivation, nor cross-inhibition alone are suf-...