A5059347003- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Transgenic Plants and Applications
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Genomics and Phylogenetic Studies
- Tissue Engineering and Regenerative Medicine
- Graphene and Nanomaterials Applications
- Cancer Research and Treatments
- Pediatric health and respiratory diseases
National Cancer Institute
2023
National Institutes of Health
2023
Instituto de Biología Molecular y Celular de Rosario
2016-2020
National University of Rosario
2016-2020
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2020
Summary The stringent response is a universal adaptive mechanism to protect bacteria from nutritional and environmental stresses. role of the during lipid starvation has been studied only in Gram‐negative bacteria. Here, we report that also plays crucial adaptation model Gram‐positive Bacillus subtilis fatty acid starvation. B. lacking all three (p)ppGpp‐synthetases (Rel Bs , RelP RelQ) or bearing Rel variant no longer synthesizes (p)ppGpp suffer extreme loss viability on Loss paralleled by...
Delivery of cancer therapeutics to non-specific sites decreases treatment efficacy while increasing toxicity. In ovarian cancer, overexpression the cell surface marker HER2, which several target, relates poor prognosis. We recently reported assembly biocompatible bacterial spore-like particles, termed "SSHELs." Here, we modify SSHELs with an affibody directed against HER2 and load them chemotherapeutic agent doxorubicin. Drug-loaded reduce tumor growth increase survival lower toxicity in a...
Peptide-based therapeutic immunizations represent safe approaches to elicit antigen-specific T cell responses, but their broad utility remains limited due poor immunogenicity and short in vivo stability rapid degradation clearance. Here we employed synthetic bacterial spore-like particles, "SSHELs", made entirely of biocompatible materials, deliver a model peptide antigen the absence additional adjuvants. SSHELs carrying were internalized by dendritic cells SSHEL-delivered peptides then...
PlsX is the first enzyme in pathway that produces phosphatidic acid Gram-positive bacteria. It makes acylphosphate from acyl-acyl carrier protein (acyl-ACP) and also involved coordinating phospholipid fatty biosyntheses. a peripheral membrane Bacillus subtilis, but how it associates with remains largely unknown. In present study, using fluorescence microscopy, liposome sedimentation, differential scanning calorimetry, acyltransferase assays, we determined binds directly to lipid bilayers...
A key aspect in membrane biogenesis is the coordination of fatty acid to phospholipid synthesis rates. In most bacteria, PlsX first enzyme phosphatidic pathway, common precursor all phospholipids. Previously, we proposed that a regulatory point synchronizes synthase II with Bacillus subtilis. However, understanding basis such mechanism remained challenge Gram-positive bacteria. Here, show inhibition and caused by depletion leads accumulation long-chain acyl-ACPs, end products II. Hydrolysis...