A5079014789- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Plant responses to elevated CO2
- Photosynthetic Processes and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Light effects on plants
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant Stress Responses and Tolerance
- Chronic Lymphocytic Leukemia Research
- Atmospheric chemistry and aerosols
- Lichen and fungal ecology
- Blood groups and transfusion
- Reproductive System and Pregnancy
- Tryptophan and brain disorders
- IL-33, ST2, and ILC Pathways
- Plant Water Relations and Carbon Dynamics
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Antioxidant Activity and Oxidative Stress
University of Kentucky HealthCare
2024
University of Kentucky
2013-2023
Markey Cancer Center
2005-2021
Universität Innsbruck
2003-2015
Czech Academy of Sciences, Institute of Botany
2007
University of Iowa
1994-2005
Utah State University
2000
Oregon Health & Science University
1998
University of Minnesota
1998
University of Wisconsin–Madison
1998
Programmed cell death (PCD) is a key element in normal plant growth and development which may also be induced by various abiotic biotic stress factors including salt stress. In the present study, morphological, biochemical, physiological responses of theoretically immortal unicellular freshwater green alga Micrasterias denticulata were examined after (200 mM NaCl or 200 KCl) osmotic iso-osmotic sorbitol. KCl caused morphological changes such as cytoplasmic vacuolization, extreme deformation...
ABSTRACT Selected high alpine plant species were collected from different elevations in the Obergurgl/Ötztal subnival and nival regions Austria to investigate content of antioxidants plants growing under particular vivo conditions experienced this area (e.g. chilling stress, short vegetation period irradiation). The contents (ascorbic acid, tocopherol glutathionc) photosynthetic pigments measured throughout day. most compounds found follow a diurnal rhythm, with maximum occurring at midday...
Killer immunoglobulin-like receptors (KIR) bind self-major histocompatibility complex class I molecules, allowing natural killer (NK) cells to recognize aberrant that have down-regulated I. NK express variable numbers and combinations of highly homologous clonally restricted KIR genes, but uniformly KIR2DL4. We show clones both 2DL4 alleles either one or the 3DL1 3DL2 genes. Despite allele-independent expression, differed in core promoter by only two nucleotides. Allele-specific gene...
MicroRNAs (miRs) have recently been identified as important regulators of gene expression at the posttranscriptional level. Although it has clearly established that miRs influence ontogeny several immune cell lineages, role individual during NK development not described. In this study, we show miR-181 levels a profound impact on human cells from CD34(+) hematopoietic progenitor and IFN-γ production in primary CD56(+) cells. We also demonstrate nemo-like kinase (NLK), an inhibitor Notch...
Abstract Human NK cells lyse Ab-coated target through the process of Ab-dependent cellular cytotoxicity (ADCC). Improving ADCC responses is desirable because it thought to be an important antitumor mechanism for some Abs. cell inhibitory receptors, such as killer Ig-like engage with MHC class I molecules on self-cells block activation. Accordingly, we enhanced by blocking thus perturbing induction self-recognition signal. In a line model anti-lymphoma therapy, combination rituximab Ab that...
In comparison with HLA-A and -B, the protein products of HLA-C locus are poorly characterized, in part because their low level expression at cell surface. Here, we examine how protein-protein interactions during assembly regulation mRNA affect surface HLA-C. We find that intrinsic properties heavy chain proteins do not correlate expression: chains associate dissociate beta 2-microglobulin (beta 2m) rates comparable to those found for increased competition 2m does alter From studies chimeric...
Abstract NK cells are essential for health, yet little is known about human turnover in vivo. In both young and elderly women, all subsets proliferated died more rapidly than T cells. CD56bright but relatively slowly, suggesting that proliferating differentiate into CD56dim The relationship between indicates self-renew derived from Our data suggest some dying become CD16+CD56− CD16−CD56low respond to cellular cytokine stimulation. We propose a model which cell dynamic flux. About half of...
Background: It has been suggested that certain medical conditions and drug exposures might suppress the immune system increase risk of developing non-Hodgkin's lymphoma (NHL). Purpose: We investigated whether specific were associated with NHL in a cohort older women who enrolled Iowa Women's Health Study. Methods: A 41 837 women, 55–69 years age at baseline, was followed prospectively for development cancer from 1986 through 1992. These had completed baseline questionnaire January inquired...
Background. Epstein-Barr virus (EBV) is maintained as an episome in most infected cells. The presence of fused terminal restriction enzyme fragments distinguishes the circular DNA form from linear virion form. Methods. EBV genomic structure was analyzed 8 lymphoid cell lines and 21 human lymphoma specimens by Southern blot technique. Results. Evidence viral integration into host chromosomal identified four lines. In Namalwa BL30-B95.8 lines, occurred through repeat (TR) sequences. BL41-P3HR1...
Abstract Mature human NK lymphocytes express the highly homologous killer Ig-like receptor (KIR) genes in a stochastic fashion, and KIR transcription precisely correlates with allele-specific DNA methylation. In this study, we demonstrate that CpG methylation of minimal promoter inhibited transcription. peripheral blood cells long-term cell lines, expressed were associated moderate level acetylated histone H3 H4 trimethylated lysine 4. Histone modifications preferentially transcribed allele...
Abstract Killer lymphocytes recognize stress-activated NKG2D ligands on tumors. We examined ligand expression in head and neck squamous cell carcinoma (HNSCC) cells other lines. HNSCC typically expressed MHC class I chain-related gene A (MICA), MICB, UL16-binding protein (ULBP)2, ULBP3, but they were uniformly negative for surface ULBP1 ULBP4. then studied how cancer treatments affected expression. was not changed by most cancer-relevant treatments. However, bortezomib proteasome inhibitor...
ABSTRACT Two clones of 5‐year‐old Norway spruce [ Picea abies (L.) Karst.] were exposed to two atmospheric concentrations CO2 (350 and 750 μmol mol −1 ) O 3 (20 75nmolmol in a phytotron at the GSF‐Forschung‐szentrum (Munich) over course single season (April October). The was programmed recreate an artificial climate similar that high elevation site Inner Bavarian Forest, trees grown large containers forest soil fertilized achieve contrasting levels potassium nutrition, designated...
We investigated the relation of alcohol consumption to risk non-Hodgkin's lymphoma (NHL) in a cohort 35 156 Iowa women aged 55-69 years who participated Women's Health Study 1986. Alcohol at baseline was obtained using mailed questionnaire. During 9-year follow-up period, 143 incident cases NHL were identified. Higher significantly associated with decreased (P-trend = 0.03). Compared non-drinkers, multivariate-adjusted relative risks (RRs) for intake < or 3.4 g day(-1) (RR 0.78; 95%...
Abstract: In radiation exposure experiments, the effects of mild artificial UV conditions (4.7 W m ‐2 UV‐A and 0.20 UV‐B) plus PAR (25 ‐ 30 μmol photons s ‐1 ) on photosynthesis ultrastructure two red algal species from Arctic have been investigated. While Palmaria palmata was collected upper sublittoral Kongsfjord (Spitsbergen, Norway), Odonthalia dentata represents a typical deepwater at this high latitude. After 6 h 24 to UV, chlorophyll fluorescence photosystem II (PS efficiency, F v /F...