A5004207372- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Light effects on plants
- Plant nutrient uptake and metabolism
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Traumatic Brain Injury and Neurovascular Disturbances
- Autophagy in Disease and Therapy
- Aluminum toxicity and tolerance in plants and animals
- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Plant tissue culture and regeneration
- RNA modifications and cancer
Duke University
2021-2025
Washington University in St. Louis
2018-2022
Living Systems (United States)
2020-2021
University of Georgia
2015-2020
University of South Florida
2014
Georgetown University Medical Center
2013
Georgetown University
2013
The plant hormones strigolactones and smoke-derived karrikins are butenolide signals that control distinct aspects of development. Perception both molecules in Arabidopsis thaliana requires the F-box protein MORE AXILLARY GROWTH2 (MAX2). Recent studies suggest homologous SUPPRESSOR OF MAX2 1 (SMAX1) DWARF53 (D53) rice (Oryza sativa) downstream targets MAX2. Through an extensive analysis loss-of-function mutants, we demonstrate SMAX1-LIKE genes SMXL6, SMXL7, SMXL8 co-orthologs D53 promote...
Karrikins (KARs) are butenolides found in smoke that can influence germination and seedling development of many plants. The KAR signaling mechanism is hypothesized to be very similar the plant hormone strigolactone (SL). Both pathways require F-box protein MORE AXILLARY GROWTH2 (MAX2), other core components have shared ancestry. Putatively, activates receptor KARRIKIN INSENSITIVE2 (KAI2), triggering its association with E3 ubiquitin ligase complex SCFMAX2 downstream targets SUPPRESSOR OF...
Abstract Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates activity, resulting in dramatic shifts auxin landscape. Here, we perform a forward genetics screen for hypercondensation, identifying an F-box protein, which named F-BOX1 (AFF1). Functional characterization SCF AFF1 revealed that this E3 ubiquitin ligase directly...
The Arabidopsis thaliana DREB2A transcription factor interacts with the negative regulator RCD1 and ACID domain of subunit 25 transcriptional co-regulator mediator (Med25) to integrate stress signals for gene expression, elusive molecular interplay. Using biophysical structural analyses together high-throughput screening, we reveal a bivalent binding switch in containing an ACID-binding motif (ABS) known RCD1-binding (RIM). RIM is lacking stress-induced splice variant retained activity. ABS...
Soluble amyloid-beta (Aβ) oligomers are hypothesized to be the pathogenic species in Alzheimer's disease (AD), and increased levels of brain subsequent traumatic injury (TBI) may exacerbate secondary pathways underlie risk developing AD later life. To determine whether TBI causes Aβ aggregation oligomerization brain, we exposed triple transgenic model mice controlled cortical impact measured soluble, insoluble, oligomeric by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7 days...
Photoactivated phytochrome B (PHYB) binds to antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) regulate hundreds of light responsive genes in Arabidopsis by promoting PIF degradation. However, whether PHYB directly controls the transactivation activity PIFs remains ambiguous. Here we show that prototypic PIF, PIF3, possesses a p53-like activation domain (AD) consisting hydrophobic activator motif flanked acidic residues. A PIF3mAD mutant, which is replaced with...
Protein neddylation is involved in a wide variety of cellular processes. Here we show that the DNA damage response perturbed cells inactivated with an E2 Nedd8 conjugating enzyme UBE2M, measured by RAD51 foci formation kinetics and cell based repair assays. UBE2M knockdown increases breakages sensitivity to damaging agents, further suggesting heightened genomic instability defective activity. Investigating downstream Cullin targets revealed silencing 1, 2, 4 ligases incurred significant...
Abstract Transcription factors carry long intrinsically disordered regions often containing multiple activation domains. Despite numerous recent high‐throughput identifications and characterizations of domains, the interplay between sequence motifs, regulator binding in transcription factor remains unresolved. Here, we map motifs domains an Arabidopsis thaliana NAC clade, revealing that although coincide, no systematic overlap exists. Biophysical analyses using NMR spectroscopy show region...
Gene expression is induced by transcription factors (TFs) through their activation domains (ADs). However, ADs are unconserved, intrinsically disordered sequences without a secondary structure, making it challenging to recognize and predict these regions limiting our ability identify TFs. Here, we address this challenge leveraging neural network approach systematically ADs. As input for network, used computed properties amino acid (AA) side chain rather than relying on the raw sequence....
The phytohormone auxin regulates nearly every aspect of plant development. Transcriptional responses to are driven by the activities AUXIN RESPONSE FACTOR family transcription factors. ARF19 (AT1G19220) is critical in signaling pathway and has previously been shown undergo protein condensation tune root. However, dynamics other organs not yet described. In Arabidopsis stomatal lineage, we found that cytoplasmic condensates enriched guard cells pavement cells, terminally differentiated leaf...
Abstract The Arabidopsis thaliana DREB2A transcription factor interacts with the negative regulator RCD1 and ACID domain of subunit 25 transcriptional activator mediator (Med25) to integrate stress signals for gene expression, elusive molecular interplay. Using biophysical structural analyses together high-throughput screening, we revealed a bivalent binding switch in containing novel ACID-binding motif (ABS) RCD1-binding (RIM). RIM is lacking stress-induced splice variant retained activity....
A major challenge in plant biology is to understand how the hormone auxin regulates diverse transcriptional responses throughout development, different environments, and species. The answer may lie specific complement of signaling components each cell. balance between activators (class-A AUXIN RESPONSE FACTORS) repressors (class-B ARFs) particularly important. It unclear this achieved. Through comparative analysis novel, dominant mutants maize moss Physcomitrium patens , we have discovered a...
ABSTRACT Auxin critically regulates nearly every aspect of plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family transcription factors. Although ARF protein stability is regulated via 26S proteasome, molecular mechanisms underlying turnover unknown. Here, we report identification functional characterization an F-box E3 ubiquitin ligase, which have named F-BOX1 (AFF1). AFF1 directly interacts with ARF19 its...
ABSTRACT Phytochrome B (PHYB) triggers diverse light responses in Arabidopsis by binding to a group of antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) promote PIF degradation, consequently downregulating target genes. However, whether PHYB directly controls the transactivation activity PIFs remains ambiguous. Here we show that prototypic PIF, PIF3, possesses p53-like activation domain (TAD) consisting sequence-specific, hydrophobic activator motif surrounded...