A5102985095- Monoclonal and Polyclonal Antibodies Research
- Mycotoxins in Agriculture and Food
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Microbial infections and disease research
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- Listeria monocytogenes in Food Safety
- Molecular Biology Techniques and Applications
- Fungal and yeast genetics research
- bioluminescence and chemiluminescence research
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Structural Characterization
- vaccines and immunoinformatics approaches
- Chemical Synthesis and Analysis
- Metabolomics and Mass Spectrometry Studies
- 14-3-3 protein interactions
- Melamine detection and toxicity
- Indoor Air Quality and Microbial Exposure
- Geochemistry and Elemental Analysis
State Key Laboratory of Food Science and Technology
2014-2025
Nanchang University
2014-2025
Jiangxi University of Technology
2025
University of Michigan
2020
Education Department of Jiangxi Province
2007
Phage display-mediated immuno-polymerase chain reaction (PD-IPCR) is an ultrasensitive detection technology that combines the advantages of immuno-PCR and phage display. The particle, which displayed antibody fragments including single-chain fragment variable (scFv), domain heavy-chain antibodies (VHH), antigen-binding (Fab) on surface can be directly used in IPCR, supplying both deoxyribonucleic acid (DNA) template. In this work, we ochratoxin A (OTA) as a model system to study capacity...
To facilitate prostate cancer imaging using targeted molecules, we constructed ultrasonic nanobubbles coupled with specific anti-PSMA (prostate membrane antigen) nanobodies, and evaluated their in vitro binding capacity vivo efficacy. The "targeted" nanobubbles, which were via a biotin-streptavidin system, had an average diameter of 487.60 ± 33.55 nm carried the nanobody as demonstrated by immunofluorescence. Microscopy revealed to PSMA-positive cells. Additionally, ultrasonography...
Nanoluciferase (Nluc), the smallest luciferase known, was used as fusion partner with a nanobody against aflatoxin B1 to develop bioluminescent enzyme immunoassay (BLEIA) for detection of in cereal. Nanobody (clone G8) fused nanoluciferase and cloned into pET22b expression vector, then transformed Escherichia coli. The gene contained hexahistidine tag purification by immobilized metal affinity chromatography, yielding biologically active protein. protein G8-Nluc retained binding properties...
Summary Heavy‐chain‐only antibodies (HCAbs), which are devoid of light chains, have been found naturally occurring in various species including camelids and cartilaginous fish. Because their high thermostability, refoldability capacity for cell permeation, the variable regions heavy chain HCAbs (VHHs) widely used diagnosis, bio‐imaging, food safety therapeutics. Most immunogenetic functional studies based on case or a limited number low‐throughput sequencing data. A complete picture derived...
Deoxynivalenol (DON), or vomitoxin, is a mycotoxin of the trichothecene group produced by Fusarium species. The present work described construction phage display library based on variable domain heavy chain HCAbs (VHHs) and panning for binders DON. Two pair PCR primers designed VHHs conserved domains were used cloning coding sequences complementary DNA (cDNA). After total RNA extraction, semi-nested PCR, subcloning electro transformation, primary was generated containing 1.9×107 independent...
Aflatoxin B
This study is designed to address the development, synthesis, and screening of non-animal-derived nanoantibody libraries. Furthermore, it seeks elucidate impact framework region selection complementarity-determining (CDR) design on characteristics synthesized These investigations aim establish a robust theoretical technical foundation for enhancing efficacy, diversity, practical applicability synthetic In this study, new (IGHV3S65*01-IGHJ4*01) was identified based high-throughput sequencing...
Anti-idiotypic nanobodies (AId-Nbs) are novel antigens that can replace the conventional hapten–protein conjugates of small molecules toxin, serving same function in competitive immunoassay.
Magnetic beads with poly(acrylic acid) brushes as “nanobody containers” for aflatoxin B<sub>1</sub> immunoaffinity purification.