A5060996854- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Monoclonal and Polyclonal Antibodies Research
- Allergic Rhinitis and Sensitization
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Microbial Natural Products and Biosynthesis
- Immune responses and vaccinations
- Cytomegalovirus and herpesvirus research
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Diabetes and associated disorders
- Adrenal Hormones and Disorders
- Acoustic Wave Resonator Technologies
- Sinusitis and nasal conditions
- Autonomous Vehicle Technology and Safety
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Eosinophilic Esophagitis
- Viral Infections and Outbreaks Research
- Thyroid Disorders and Treatments
- Plant Disease Resistance and Genetics
- Obstructive Sleep Apnea Research
University of California, Berkeley
2023
Jiangsu Provincial Center for Disease Control and Prevention
2023
Lawrence Berkeley National Laboratory
2023
Joint BioEnergy Institute
2023
King's College London
2010-2022
Asthma UK
2014-2018
ENT and Allergy
2015
King's College School
2010-2015
Medical Research Council
2014
Agriculture and Agri-Food Canada
1995
Older people suffer from a decline in immune system, which affects their ability to respond infections and raise efficient responses vaccines. Effective specific antibodies older individuals are decreased favour of non-specific antibody production. We investigated the B-cell repertoire DNA samples peripheral blood aged 86-94 years, control group 19-54 using spectratype analysis IGHV complementarity determining region (CDR)3. found that proportion had dramatic collapse diversity. Sequencing...
CD27 expression has been used to distinguish between memory and naive B cells in humans. However, low levels of mutated isotype-switched CD27-IgD- are seen healthy adults, these increased some autoimmune diseases the elderly. Thus is not a universal marker Various hypotheses have put forward as function CD27- population. Since we previously found high throughput IGHV repertoire analysis useful "innate-like" (CD27+IgD+), employed similar analyses elucidate relationship CD27+ cells. IgM+IgD-...
It is well known that older people are more susceptible to morbidity and mortality from infectious diseases, particularly pulmonary diseases such as pneumococcal pneumonia where vaccines do not provide efficient protection in younger populations. We have previously shown the B-cell repertoire old reduced hypothesise this may contribute impaired humoral responses of elderly. Here, we investigated antibody winter vaccination two age groups, aged 18-49 65-89. found serum IgM IgA were...
Human memory B cells and marginal zone (MZ) share common features such as the expression of CD27 somatic mutations in their IGHV BCL6 genes, but relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues MZ emerge from mature naïve cell pool via a precursor
A molecular linkage map of cultivated oat composed 561 loci has been developed using 71 recombinant inbred lines from a cross between Avena byzantina cv. Kanota and A. sativa Ogle. The are mainly restriction fragment length polymorphisms detected by cDNA clones leaf, endosperm, root tissue, as well barley leaf clones. form 38 groups ranging in size 0.0 to 122.1 cM (mean, 39 cM) consist 2-51 each 14). Twenty-nine remain unlinked. current is 1482 the total size, on basis number unlinked loci,...
Immune protection against pulmonary infections, such as seasonal flu and invasive pneumonia, is severely attenuated with age, vaccination regimes for the elderly people often fail to elicit effective immune responses. We have previously shown that influenza pneumococcal vaccine responses in older population are significantly impaired terms of serum antibody production, repertoire differences by CDR-H3 spectratype analysis. Here we report a detailed analysis B cell response vaccine, including...
Older people are more susceptible to infection, less responsive vaccination and have a inflammatory immune environment. Using spectratype analysis, we previously shown that the B-cell repertoire of older shows evidence inappropriate clonal expansions in absence challenge, this loss diversity correlates with poor health. Studies on response vaccination, using both spectratyping high-throughput sequencing repertoire, indicate responses challenge lacking magnitude and/or delayed significantly....
Antibody variable regions are composed of a heavy and light chain in humans there two isotypes: kappa lambda. Despite their importance receptor editing, the is often overlooked antibody literature, with focus being on CDR-H3 region. In this paper, we set out to investigate physicochemical structural differences between human lambda CDR regions. We constructed dataset containing over 29,000 - region sequences from IgM-transcribing, newly formed B cells isolated bone marrow peripheral blood....
The B cell repertoire is generated in the adult bone marrow by an ordered series of gene rearrangement processes that result massive diversity immunoglobulin (Ig) genes, and consequently equally large number potential specificities for antigen. As process essentially random, then cells exhibiting excess reactivity with self-antigens are need to be removed from before fully mature. Some deleted, some will undergo receptor editing see if changing light chain can rescue autoreactive antibody. a...
Previous studies of immunoglobulin gene sequences in patients with allergic diseases using low-throughput Sanger sequencing have limited the analytic depth for characterization IgE repertoires.We used a high-throughput, next-generation approach to characterize heavy-chain (IGH) repertoires seasonal rhinitis (AR) aim better understanding underlying disease mechanisms.IGH matched peripheral blood and nasal biopsy specimens from nonallergic healthy control subjects (n = 3) grass pollen-related...
Chronic rhinosinusitis with nasal polyps is associated local immunoglobulin hyperproduction and the presence of IgE antibodies against Staphylococcus aureus enterotoxins (SAEs). Aspirin-exacerbated respiratory disease a severe form chronic in which nearly all patients express anti-SAEs.We aimed to understand reactive SAEs determine whether they recognize through their complementarity-determining regions (CDRs) or framework regions.Labeled staphylococcal enterotoxin (SE) A, SED, SEE were used...
Rationale: Type 2 innate lymphoid cells (ILC2s) are significant sources of type cytokines, which implicated in the pathogenesis asthma and exacerbations. The role ILC2s virus-induced exacerbations is not well characterized. Objectives: To characterize pulmonary ILC responses following experimental rhinovirus challenge patients with moderate healthy subjects. Methods: Patients subjects were inoculated rhinovirus-16 underwent bronchoscopy at baseline Day 3, 8 after inoculation. Pulmonary ILC1s...
Monoterpenes are commonly known for their role in the flavors and fragrances industry also gaining attention other uses like insect repellant as potential renewable fuels aviation. Corynebacterium glutamicum, a Generally Recognized Safe microbe, has been choice organism annual million ton-scale bioproduction of amino acids more than 50 years; however, efforts to produce monoterpenes C. glutamicum have remained relatively limited. In this study, we report further expansion biosynthetic...
The CD27 + IgD B cell population, known as IgM memory, reduces with age. It is thought that this population responsible for pneumococcal polysaccharide T‐independent responses, and the age‐related reduction might be partially increased susceptibility of older people to bacterial pathogens. There are other populations do not express IgD. We compared different using high‐throughput sequencing immunoglobulin (Ig) gene repertoire multidimensional phenotyping found cells, defined by expression,...
Autonomous driving cars need highly complex hardware and software systems, which require high performance computing platforms in order to enable a real time AI-based perception decision making pipeline. The industry has been exploring various in-vehicle accelerators such as GPUs, ASICs FPGAs. Yet the autonomous platform design is far from mature when taking into account of system reliability, redundancy higher level autonomy. In this work, we propose hybrid design, integrates GPU primary...
Immunoglobulin gene heterogeneity reflects the diversity and focus of humoral immune response towards different infections, enabling inference B cell development processes. Detailed compositional lineage analysis long read IGH repertoire sequencing, combining examples pandemic, epidemic endemic viral infections with control vaccination samples, demonstrates general responses including increased use