A5030497088- Genomics and Chromatin Dynamics
- Heat shock proteins research
- Prion Diseases and Protein Misfolding
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Heme Oxygenase-1 and Carbon Monoxide
- Protist diversity and phylogeny
- Neuroscience of respiration and sleep
- Toxin Mechanisms and Immunotoxins
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Thermal Regulation in Medicine
- Trace Elements in Health
- Plant Molecular Biology Research
Stanford University
2022-2024
Stanford Medicine
2023-2024
Lafayette College
2018-2021
Abstract In eukaryotes, an Hsp70 molecular chaperone triad assists folding of nascent chains emerging from the ribosome tunnel. fungi, consists canonical Ssb, atypical Ssz1 and J-domain protein cochaperone Zuo1. Zuo1 binds at tunnel exit. also Ssz1, tethering it to ribosome, while its stimulates Ssb’s ATPase activity drive efficient chain interaction. But function how Ssb engages are not well understood. Employing in vivo site-specific crosslinking, we found that Ssb(ATP) heterodimerizes...
Summary The amyloid‐based prions of Saccharomyces cerevisiae are heritable aggregates misfolded proteins, passed to daughter cells following fragmentation by molecular chaperones including the J‐protein Sis1, Hsp70 and Hsp104. Overexpression Hsp104 efficiently cures cell populations prion [ PSI + ] an alternative Sis1‐dependent mechanism that is currently subject significant debate. Here, we broadly investigate role J‐proteins in this process determining impact amyloid polymorphisms (prion...
Abstract While population level analyses reveal significant roles for CTCF and cohesin in mammalian genome organization, their contribution to chromatin structure gene regulation at the single-cell remain incompletely understood 1–4 . Here, we use a super-resolution microscopy approach, Optical Reconstruction of Chromatin Architecture (ORCA) 5 measure effects removal or on folding across genomic scales. In untreated embryonic stem cells, observe intricate, frequently stacked loops which are...
Abstract During cell division, the genome of each eukaryotic is copied by thousands replisomes – large protein complexes consisting several dozen proteins. Recent studies suggest that replisome much more dynamic than previously thought. To directly visualize dynamics in a physiological context, we recently developed single-molecule approach for imaging replication proteins Xenopus egg extracts. These extracts contain all soluble nuclear and faithfully recapitulate DNA repair vitro , serving...
Metazoan genomes are copied bidirectionally from thousands of replication origins. Replication initiation entails the assembly and activation two CMG (Cdc45•Mcm2-7•GINS) helicases at each origin. This requires several firing factors (including TopBP1, RecQL4, DONSON) whose exact roles remain unclear. How correctly assembled activated every single origin is a long-standing question. By visualizing recruitment GINS, Cdc45, DONSON in real time, we uncovered surprisingly dynamic picture...
The amyloid‐based prions of Saccharomyces cerevisiae are heritable aggregates misfolded protein, passed to daughter cells following fragmentation by a set molecular chaperones which includes the J‐protein Sis1, Hsp70, and Hsp104. Overexpression Hsp104 efficiently cures prion [ PSI + ], phenomenon has promoted exploration as potential therapeutic agent for neurodegenerative diseases. However, mechanism ] elimination overexpression been subject significant debate past two decades garnered...