A5037536207- Neuroscience and Neuropharmacology Research
- Amino Acid Enzymes and Metabolism
- Ion channel regulation and function
- Bacterial Genetics and Biotechnology
- Cellular transport and secretion
- Microbial Community Ecology and Physiology
- Axon Guidance and Neuronal Signaling
- Metabolism and Genetic Disorders
- Crystal structures of chemical compounds
- Force Microscopy Techniques and Applications
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Crystallography and molecular interactions
- Hippo pathway signaling and YAP/TAZ
- Iron Metabolism and Disorders
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Neurogenesis and neuroplasticity mechanisms
- Neurotransmitter Receptor Influence on Behavior
- ATP Synthase and ATPases Research
- Biochemical Analysis and Sensing Techniques
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
Vilnius University
2024
MRC Laboratory of Molecular Biology
2020-2021
Aarhus University
2014-2020
Abstract Bacterial members of the neurotransmitter:sodium symporter (NSS) family perform Na + -dependent amino-acid uptake and extrude H in return. Previous NSS structures represent intermediates /substrate binding or intracellular release, but not inward-to-outward return transition. Here we report crystal Aquifex aeolicus LeuT an outward-oriented, - substrate-free state likely to be -occluded. We find a remarkable rotation conserved Leu25 into empty substrate-binding pocket rearrangements...
Abstract The three-dimensional positions of atoms in protein molecules define their structure and provide mechanistic insights into the roles they perform complex biological processes. more precisely atomic coordinates are determined, chemical information can be derived knowledge about function may inferred. With breakthroughs electron detection image processing technology, cryo-microscopy (cryo-EM) single-particle analysis has yielded structures with increasing levels detail recent years...
During cell migration or differentiation, surface receptors are simultaneously exposed to different ligands. However, it is often unclear how these extracellular signals integrated. Neogenin (NEO1) acts as an attractive guidance receptor when the Netrin-1 (NET1) ligand binds, but mediates repulsion via repulsive molecule (RGM) Here, we show that signal integration occurs through formation of a ternary NEO1-NET1-RGM complex, which triggers reciprocal silencing downstream signaling. Our...
CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for regulation plasmid competition. To understand how the type system associated effector complex achieves interference, we determine cryo-EM structures two evolutionarily distinct complexes (types IV-A1 IV-A3) bound...
Abstract MhsT of Bacillus halodurans is a transporter hydrophobic amino acids and homologue the eukaryotic SLC6 family Na + -dependent symporters for acids, neurotransmitters, osmolytes, or creatine. The broad range transported by prompted investigation substrate recognition mechanism. Here, we report six new substrate-bound structures MhsT, which, in conjunction with functional studies, reveal how flexibility Gly-Met-Gly (GMG) motif unwound region transmembrane segment 6 (TM6) central...