A5049009941- Phosphodiesterase function and regulation
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Inflammatory mediators and NSAID effects
- PARP inhibition in cancer therapy
- Medicinal plant effects and applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer Mechanisms and Therapy
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Synthesis and biological activity
- Cancer Treatment and Pharmacology
- Chemical Synthesis and Analysis
- Nutrition and Health in Aging
- Asymmetric Hydrogenation and Catalysis
- Molecular Biology Techniques and Applications
- Protein Degradation and Inhibitors
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Endometriosis Research and Treatment
- Carcinogens and Genotoxicity Assessment
- Cancer Risks and Factors
- Signaling Pathways in Disease
- Academic Writing and Publishing
- Cancer, Stress, Anesthesia, and Immune Response
University of South Alabama
2014-2025
USA Mitchell Cancer Institute
2014-2025
Brookwood Baptist Health
2019
Mayo Clinic in Florida
2018
WinnMed
2018
University of Georgia
2016
Georgia Institute of Technology
2012-2014
AID Atlanta
2012
MedStar Washington Hospital Center
2010
University of Wisconsin–Madison
1999-2000
Troponin I is a subunit of the thin filament-associated troponin-tropomyosin complex involved in calcium regulation skeletal and cardiac muscle contraction. We deleted isoform troponin by using gene targeting murine embryonic stem cells to determine developmental physiological effects absence this regulatory protein. Mice lacking were born healthy, with normal heart body weight, because fetal (identical slow I) compensated for I. Compensation was only temporary, however, as 15 days after...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA molecular mechanics model of ligand effects. 2. Binding phosphines to pentacarbonylchromiumKevin J. Lee and Theodore L. BrownCite this: Inorg. Chem. 1992, 31, 2, 289–294Publication Date (Print):January 1, 1992Publication History Published online1 May 2002Published inissue 1 January 1992https://pubs.acs.org/doi/10.1021/ic00028a030https://doi.org/10.1021/ic00028a030research-articleACS PublicationsRequest reuse permissionsArticle...
DNA repair defects have been increasingly focused on as therapeutic targets. In hormone-positive breast cancer, XRCC1-deficient tumors identified and proposed targets for combination therapies that damage inhibit pathways. XRCC1 is a scaffold protein functions in base excision (BER) by mediating essential interactions between glycosylases, AP endonuclease, poly(ADP-ribose) polymerase 1, β (POL β), ligases. Loss of confers BER hypersensitivity to damaging agents. not evaluated triple negative...
Dihydronicotinamide rioside (NRH), the reduced form of nicotinamide riboside (NR), is a recently identified, naturally occurring precursor arguably most crucial cofactor for cellular function, adenine dinucleotide (NAD+). Recent investigation suggests that NRH more adept at increasing NAD+ stores than traditional precursors, and such extreme boosting via supplementation induces cytotoxicity in certain contexts. It has also been shown lack functional BRCA protein epithelial ovarian cancer...
Abstract Background: Recent evidence shows that G-protein-coupled receptor-171 (GPR171) may be an important T-cell checkpoint in tumor immunity. The role of GPR171 cervical cancer has not been studied. Here, we sought to investigate the association with other immune markers and DNA damage its impact on patient survival. Methods: 3,371 samples were sequenced for RNA (NovaSeq, WTS) (Caris Life Sciences, Phoenix, AZ). GPR171, CD274, CD96 TIGIT stratified by top 25% (-H) bottom 75% (-L)....
We describe a multi-omic approach to understanding the effects that anti-malarial drug pyrimethamine has on immune physiology in rhesus macaques (Macaca mulatta). Whole blood and bone marrow RNA-Seq plasma metabolome profiles (each with over 15,000 features) have been generated for five naïve individuals at up seven time-points before, during after three rounds of administration. Linear modelling Bayesian network analyses are both considered, alongside investigations impact statistical...
African Americans (AA) are two times more likely to be diagnosed with and succumb prostate cancer (PCa) compared European (EA). There is mounting evidence that biological differences in these tumors contribute disparities patient outcomes. Our goal was examine the DNA damage AA EA tissues. Tissue microarrays matched tumor-benign adjacent pairs from 77 PCa patients were analyzed for abasic sites, oxidative lesions, crosslinks, uracil content using Repair Assisted Damage Detection (RADD)...
A leading theory for ovarian carcinogenesis proposes that inflammation associated with incessant ovulation is a driver of oncogenesis. Consistent this theory, nonsteroidal anti-inflammatory drugs (NSAIDs) exert promising chemopreventive activity cancer. Unfortunately, toxicity long-term use NSAIDs due to their cyclooxygenase (COX) inhibitory activity. Previous studies suggest the antineoplastic COX independent, and rather may be exerted through phosphodiesterase (PDE) inhibition. PDEs...
We report the first whole-genome sequences for five strains, two carried and three pathogenic, of emerging pathogen Haemophilus haemolyticus. Preliminary analyses indicate that these genome encode markers distinguish H. haemolyticus from its closest relatives provide clues to identity virulence factors.
Abstract Previous studies have reported that phosphodiesterase 10A (PDE10) is overexpressed in colon epithelium during early stages of tumorigenesis and essential for cancer cell growth. Here we describe a novel non-COX inhibitory derivative the anti-inflammatory drug, sulindac, with selective PDE10 activity, ADT 061. 061 potently inhibited growth cells expressing high levels PDE10, but not normal colonocytes do express PDE10. The concentration range by which was identical to concentrations...
Exposures to genotoxic carcinogens and reactive species result in strand breaks a spectrum of covalent modifications DNA that can induce mutations contribute the initiation progression cancer. Measurements damage within tissue or tumor samples serve as biomarker for exposures assess changes repair capacity relevant cancer development treatment. Numerous methods characterize exist. However, these are primarily applicable isolated cultured cells, often require substantial amount material, may...
The reprogramming of cellular memory in specific cell types, and visceral adipocytes particular, appears to be a fundamental aspect obesity its related negative health outcomes. We explored the hypothesis that adipose tissue contains epigenetically distinct subpopulations are differentially potentiated record memories their environment. Adipocytes large, fragile, technically difficult efficiently isolate fractionate. developed fluorescence nuclear cytometry (FNC) activated sorting (FANS)...
Abstract DNA repair defects have been increasingly focused on as therapeutic targets. In hormone positive breast cancer, XRCC1-deficient tumors identified and proposed targets for combination therapies that damage inhibit pathways. XRCC1 is a scaffold protein functions in base excision (BER) by mediating essential interactions between glycosylases, AP endonuclease, poly(ADP-ribose) polymerase 1, β (POL β), ligases. Loss of confers BER hypersensitivity to damaging agents. not evaluated triple...
Cancer Drug Resistance is an open access journal, focusing on pharmacological aspects of drug resistance and its reversal, molecular mechanisms classes, etc. Both clinical experimental in cancer are included.
Programmed death ligand-1 (PD-L1) inhibitors are currently under investigation as a potential treatment option for ovarian cancer. Although this therapy has shown promise, its efficacy is highly variable among patients. Evidence suggests that genomic instability influences the expression of PD-L1, but little known about relationship in To examine between PD-L1 and instability, we measured DNA damage using Repair Assisted Damage Detection (RADD). We then correlated presence persistent tumor...
Abstract A series of 1,2,4‐triazolo[1,5‐ a ]pyrimidine derivatives was designed, synthesized, and screened for their phosphodiesterase (PDE 4B) inhibitory activity bronchodilation ability. Compound 7e showed 41.80% PDE 4B inhibition at 10 µM. Eight compounds were bronchodilator activity, where 7f elicited promising with EC 50 values 18.6 57.1 µM, respectively, compared to theophylline (EC = 425 µM). Molecular docking the active site revealed binding mode scores comparable those reference...
It is currently thought that small RNA (sRNA) based repression mechanisms are primarily employed to mitigate the mutagenic threat posed by activity of transposable elements (TEs). This can be achieved sRNA guided processing TE transcripts via Dicer-dependent (e.g., siRNA) or Dicer-independent piRNA) mechanisms. For example, potentially active human L1 silenced mRNA cleavage induced element encoded siRNAs, leading a negative correlation between and siRNA levels. On other hand, there emerging...
In view of the emerging clinical indications for Phosphodiesterase 9 inhibitors e.g. treatment Alzheimer, diabetes, cancer, and limited number its selective which possess a single chemical scaffolds, structure-based approach was undertaken to mine ZINC database by virtual screening identify novel PDE9 inhibitors. The database, never reported have been used before discovery inhibitors, screened against ligand binding pocket complex (PDB:4GH6) using molecular docking programs, MOE AutoDock...
This study delves into the pathogenesis of virulent genotype VII strains Newcastle disease virus (NDV), focusing on experimentally infected birds. Predominant and consistent lesions observed include bursal atrophy extensive depletion all lymphoid tissues. Immunohistochemistry (IHC) analysis, targeting apoptosis (Caspase-3), necroptosis (MLKL), NDV markers, indicates that is linked to a non-apoptotic programmed cell death pathway known as "necroptosis". Repair assisted damage detection (RADD)...