A5043424438- Collagen: Extraction and Characterization
- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Bacterial Genetics and Biotechnology
- Bone Tissue Engineering Materials
- RNA and protein synthesis mechanisms
- Nanoparticle-Based Drug Delivery
- Food Industry and Aquatic Biology
- Electron and X-Ray Spectroscopy Techniques
- Aquaculture disease management and microbiota
- Force Microscopy Techniques and Applications
- Enzyme Production and Characterization
- Iron Metabolism and Disorders
- Engineering Technology and Methodologies
- Protein Hydrolysis and Bioactive Peptides
- Porphyrin Metabolism and Disorders
- Iron oxide chemistry and applications
- Nanowire Synthesis and Applications
- Anodic Oxide Films and Nanostructures
- Ichthyology and Marine Biology
- Toxin Mechanisms and Immunotoxins
- Protein Structure and Dynamics
- Phytase and its Applications
- Magnetic Properties and Synthesis of Ferrites
- Silk-based biomaterials and applications
Voronezh State University
2015-2024
Immanuel Kant Baltic Federal University
2017-2024
Moscow State University of Technologies and Management named after K.G. Razumovskiy
2020-2021
Voronezh State University of Engineering Technologies
2020
Institute of Cell Biophysics
2014-2019
Pushchino State Institute of Natural Sciences
2014-2017
Dps is a multifunctional homododecameric protein that oxidizes Fe2+ ions accumulating them in the form of Fe2O3 within its cavity, interacts with DNA tightly condensing bacterial nucleoid upon starvation and performs some other functions. During last two decades from discovery this protein, ferroxidase activity became rather well studied, but mechanism interaction still remains enigmatic. The crucial role lysine residues unstructured N-terminal tails led to conventional point view binds...
Spinel ferrite magnetic nanoparticles have attracted considerable attention because of their high and flexible properties biocompatibility. In this work, a set cobalt doped with zinc was synthesized via the eco-friendly sol-gel auto-combustion method. Obtained particles displayed room-temperature ferromagnetic behavior tuned by chemical composition values saturation magnetization coercivity. The maximal ~74 Am2/kg were found in 15–35% molar fraction replaced ions. At same time, coercivity...
Seventy-eight promoter islands with an extraordinarily high density of potential promoters have been recently found in the genome Escherichia coli. It has shown that RNA polymerase binds internal these and produces short oligonucleotides, while synthesis normal mRNAs is suppressed. This quenching may be biologically relevant, as most are associated foreign genes, which expression deplete cellular resources. However, a molecular mechanism silencing participation promoter-rich regions remains...
In the present article, possible mitigation of toxic effect imidacloprid low-concentration chronic exposure on Danio rerio by probiotic strain Lactobacillus brevis 47f (1 × 108 CFU/g) was examined. It found that even sublethal concentration (2500 µg/L) could lead to death some fish during 60-day experiment. However, use partially reduced effects, resulting in an increased survival rate and a significant reduction morphohistological lesions intestines kidneys rerio. The were be most...
Multifunctional protein Dps plays an important role in iron assimilation and a crucial bacterial genome packaging. Its monomers form dodecameric spherical particles accumulating ~400 molecules of oxidized ions within the cavity applying flexible N-terminal ends each subunit for interaction with DNA. Deposition is well-studied process by which cells remove toxic Fe2+ from genetic material store them easily accessible form. However, mode linear DNA remained mysterious binary complexes have not...
The novelty of the work lies in creation and study physical biological properties biodegradable polymer coatings for stents based on poly(lactic-co-glycolic acid) (PLGA). Polymer are capable prolonged directed release molecules with a high molecular weight, particular, protein prourokinase (m.w. 54 kDa). A technology has been developed to create having relative elongation 40% 165% tensile strength 25-65 MPa. Coatings biodegradable; rate degradation an isotonic solution varies range...
The Dps protein of Escherichia coli, which combines ferroxidase activity and the ability to bind DNA, is effectively used by bacteria protect their genomes from damage. Both activities depend on integrity this multi-subunit protein, has an inner cavity for iron oxides; however, diversity its oligomeric forms only been studied fragmentarily. Here, we show that ions stabilize dodecameric form Dps. This was found electrophoretic fractionation size exclusion chromatography, revealed several...
In this study, the effect of three promising feed additives (chelated compounds trace elements, butyric acid, lycopene) on changes in culturable microbiota and histological parameters two sections intestines
Renewed technology has created a demand for foods which are natural in taste, minimally processed, and safe consumption. Although thermal processing, such as pasteurization sterilization, effectively limits pathogenic bacteria, it alters the aroma, flavor, structural properties of milk products. Nonthermal technologies have been used an alternative to traditional processing ability provide healthy dairy products without affecting their nutritional composition organoleptic properties. Other...
In this, work some aspects of the development visual system
Directed formation of the structure culture living cells is most important task tissue engineering. New materials for 3D scaffolds are critical mass adoption regenerative medicine protocols. In this manuscript, we demonstrate results molecular study collagen from Dosidicus gigas and reveal possibility obtaining a thin membrane material. The characterized by high flexibility plasticity as well mechanical strength. technology scaffolds, studies its properties, surface morphology, protein...
The Dps protein of Escherichia coli is a homododecamer with an internal cavity accumulating iron oxides, transformed from ambient toxic Fe2+ into harmless inorganic core. High resolution cryogenic transmission electron microscopy was applied to visualize the molecules and characterize their ability self-organization. Due ultrafast freezing, dodecameric particles were observed as "snapshot" natural state, highly ordered two-dimensional layers hexagonal symmetry obtained. Thus, it became...
The novel approach was proposed for detailed high-resolution studies of morphology and physico-chemical properties concomitantly at one measurement spot E. coli bacterial cells culture immobilized onto silicon wafer surface in UHV conditions applying PhotoEmission Electron Microscopy under Hg lamp irradiation. For the characterization scanning electron microscopy (electron beam) X-ray photoelectron spectroscopy (X-ray tube radiation) were applied prior to measurements. In spite irradiation...
Collagen is a ubiquitously expressed essential structural component of all metazoan extracellular matrices.It present in organisms ranging from sponges to humans.Although the general functions collagens are comparable, their characteristics quite distinct.There 19 different types animal collagens, as defined by tissue location.Much less known about structure hydrations compared mammal collagens.This research aimed compare sensitivity two Collagen; one hydration (silver carp,...
The Polymers Editorial Office retracts the article, "The Dosidicus gigas Collagen for Scaffold Preparation and Cell Cultivation: Mechanical Physicochemical Properties, Morphology, Composition Viability" [...].
Abstract The authors studied the possibility of using several methods for obtaining a collagen-containing base, which is collagen hydrate obtained organic acids. Collagen unique protein and part variety connective tissues living organisms. It has form three-spiral structure consisting molecule specific set amino great practical importance cosmetology medicine. one most common proteins in A significant proportion concentrated skin. Known product are associated with use acids de-fibration....
The present work is related to the microscopic studies of morphology planar and inner part silicon nanowires arrays before after immobilization with a natural nanomaterial, Dps protein bacterial origin. Silicon were formed by metal-assisted wet chemical etching. To obtain recombinant protein, Escherichia coli cells used as excretion strain purification carried out using chromatography. combination molecules was layering at laboratory conditions followed drying under air. resulting hybrid...