A5009179401- Pediatric Urology and Nephrology Studies
- Urological Disorders and Treatments
- Lipid Membrane Structure and Behavior
- Urinary Bladder and Prostate Research
- Urinary Tract Infections Management
- Artificial Intelligence in Healthcare and Education
- Antibiotic Resistance in Bacteria
- Renal cell carcinoma treatment
- Alzheimer's disease research and treatments
- Prostate Cancer Treatment and Research
- Antibiotics Pharmacokinetics and Efficacy
- Prostate Cancer Diagnosis and Treatment
- Autopsy Techniques and Outcomes
- Renal and Vascular Pathologies
- Urologic and reproductive health conditions
- Diversity and Career in Medicine
- Cardiac, Anesthesia and Surgical Outcomes
- Erythrocyte Function and Pathophysiology
- Genital Health and Disease
- Pelvic floor disorders treatments
- Bladder and Urothelial Cancer Treatments
- Ureteral procedures and complications
- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- Machine Learning in Healthcare
University of Toronto
2020-2025
Hospital for Sick Children
2021-2025
SickKids Foundation
2022-2025
Canada Research Chairs
2025
University of New Brunswick
2025
University Health Network
2023
Oxfam
2023
Liechtenstein Institute
2023
John Wiley & Sons (United States)
2023
Urological Society of Australia and New Zealand
2023
Atomic resolution map of the soluble amyloid beta assembly (Aβ<sub>n</sub>) “toxic surfaces” that facilitate early pathogenic events in Alzheimer's disease (AD).
Abstract With the advent of polymyxin B (PmB) resistance in bacteria, mechanisms for mcr -1 are crucial importance design novel therapeutics. The phenotype is known to decrease membrane charge and increase packing by modification bacterial outer membrane. We used X-ray diffraction, Molecular Dynamics simulations, electrochemistry, leakage assays determine location PmB different membranes assess damage. By varying lipid tail independently, we show that increasing surface promotes penetration...
Signaling events at membranes are often mediated by membrane lipid composition or physical properties. These properties could act either favoring the binding of downstream effectors modulating their activity. Several proteins can sense/generate curvature (i.e. shape). However, modulation activity enzymes a membrane's shape has not yet been reported. Here, using cell-free assay with purified diacylglycerol kinase ɛ (DGKɛ) and liposomes, we studied acyl-chain specificity an enzyme...
In the brain, α-synuclein (aSN) partitions between free unbound cytosolic and membrane bound forms modulating both its physiological pathological role complicating study due to structural heterogeneity. Here, we use an interdisciplinary, synergistic approach characterize properties of aSN:lipid mixtures, isolated co-structures, aSN in mammalian cells. Enabled by isolation membrane-bound state, show that within previously described N-terminal anchor, interaction relies on tail (NTT) head...
Amyloid- β aggregates play a causative role in Alzheimer's disease. These are product of the physical environment provided by basic neuronal membrane, composed lipid bilayer. The intrinsic properties bilayer allow amyloid- peptides to nucleate and form well-ordered cross- sheets within membrane. Here, we correlate aggregation hydrophobic fragment protein, A 25 - 35 , with hydrophobicity, fluidity, charge density We summarize recent biophysical studies model membranes relate these process...
Integrative structure of a complete cytokine receptor in nanodisc opens for mechanistic decoding disorder-based biology.
As more artificial intelligence (AI) applications are integrated into healthcare, there is an urgent need for standardization and quality-control measures to ensure a safe successful transition of these novel tools clinical practice. We describe the role silent trial, which evaluates AI model on prospective patients in real-time, while end-users (i.e., clinicians) blinded predictions such that they do not influence decision-making. present our experience evaluating previously developed...
Exposure to research data and artificial intelligence (AI) model predictions may lead many sources of bias in clinical decision-making evaluation. These include anchoring bias, automation leakage. In this case study, we introduce a new source termed "induced belief revision," which have discovered through our experience developing testing an AI predict obstructive hydronephrosis children based on their renal ultrasounds. After silent trial model, observed unintentional but clinically...
Objective To compare the outcomes of pre‐ vs postnatally diagnosed posterior urethral valves (PUV) at two large paediatric centres in North America to ascertain if prenatal diagnosis PUV is associated with better outcomes. Patients and Methods All boys were identified institutions between 2000 2020 (The Hospital for Sick Children [SickKids, SK] Children’s Philadelphia [CHOP]). Baseline characteristics outcome measures compared those postnatally. Main interest included progression chronic...
Caffeine partitions in lipid membranes the head to tail interface and leads a thickening defluidification.
Alpha synuclein (αS) oligomers are a key component of Lewy bodies implicated in Parkinson's disease (PD). Although primarily intracellular, extracellular αS exocytosed from neurons also contributes to PD pathogenesis through prion-like transmission mechanism. Here, we show at progressive degrees resolution that the most abundantly expressed protein, human serum albumin (HSA), inhibits oligomer (αSn) toxicity three-pronged First, endogenous HSA targets αSn with sub-μM affinity via...
No AccessJournal of UrologyPediatric Urology1 Dec 2022Multi-institutional Validation Improved Vesicoureteral Reflux Assessment With Simple and Machine Learning ApproachesThis article is commented on by the following:Editorial Comment Adree Khondker, Jethro C. Kwong, Priyank Yadav, Justin Y. H. Chan, Anuradha Singh, Marta Skreta, Lauren Erdman, Daniel T. Keefe, Katherine Fischer, Gregory Tasian, Jessica Hannick, Frank Papanikolaou, Benjamin J. Cooper, Christopher S. Mandy Rickard, Armando...