A5084451923- Cell Image Analysis Techniques
- Hearing Loss and Rehabilitation
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoacoustic and Ultrasonic Imaging
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Intensive Care Unit Cognitive Disorders
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neural Engineering
University of Iowa
2023-2025
University of Iowa Hospitals and Clinics
2022
Cochlear implants (CIs) restore hearing to deafened patients. The foreign body response (FBR) following cochlear implantation (post-CI) comprises an infiltration of macrophages, other immune and non-immune cells, fibrosis into the scala tympani, a space that is normally devoid cells. This FBR associated with negative effects on CI outcomes including increased electrode impedances loss residual acoustic hearing. study investigates extent which macrophage depletion by orally administered...
Abstract The inflammatory foreign body response (FBR) following cochlear implantation (CI) can negatively impact CI outcomes, including increased electrode impedances. This study aims to investigate the long-term efficacy of dexamethasone eluting implant and locally delivered dexamethasone, a potent anti-inflammatory glucocorticoid on intracochlear FBR electrical impedance post-implantation in murine model human subjects. left ears CX3CR1 +/GFP Thy1 +/YFP (macrophage-neuron dual reporter)...
Abstract Regular image analysis derived from confocal microscopic data of biological tissue samples with the Imaris software package involves a significant amount repetition on part researchers for each image. We developed supervised automation protocol in our lab to execute everyday cell and spot counting tasks (e.g., neuron, nucleus, macrophages) images obtained mouse cochlear samples. The proposed semi-automated can be reproduced customized by scientists working basic experimental...
Analyzing confocal microscopic data of biological samples using the Imaris software poses challenges due to its time-consuming nature involving tedious multiple steps and possibility human errors. Here, we developed a supervised automation protocol minimize manual input in cell spot counting on images obtained from mouse cochlear sections. The increases efficiency by incorporating image recognition object-oriented macros. Moreover, being adaptable allows scientists diverse other fields...
Cochlear implants (CIs) restore hearing to deafened patients. The foreign body response (FBR) following cochlear implantation (post-CI) comprises an infiltration of macrophages, other immune and non-immune cells, fibrosis into the scala tympani; a space that is normally devoid cells. This FBR associated with negative effects on CI outcomes including increased electrode impedances loss residual acoustic hearing. study investigates extent which macrophage depletion by orally administered...