A5044480763- Medicinal Plant Extracts Effects
- Genomics, phytochemicals, and oxidative stress
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Glutathione Transferases and Polymorphisms
- Colorectal Cancer Treatments and Studies
- Plant tissue culture and regeneration
- Peptidase Inhibition and Analysis
- Occupational and environmental lung diseases
- Smoking Behavior and Cessation
- Carcinogens and Genotoxicity Assessment
- Bioactive Compounds and Antitumor Agents
- Free Radicals and Antioxidants
- Synthesis of Indole Derivatives
- Cancer-related gene regulation
- Microbial Metabolites in Food Biotechnology
- Dysphagia Assessment and Management
- Bladder and Urothelial Cancer Treatments
- Nutrition and Health in Aging
- Cancer Research and Treatments
- Histone Deacetylase Inhibitors Research
- Health Literacy and Information Accessibility
- Alkaloids: synthesis and pharmacology
- Lung Cancer Diagnosis and Treatment
- Cancer therapeutics and mechanisms
University of Minnesota
2013-2024
University of Minnesota Medical Center
2012-2022
University of Minnesota, Waseca
2013
Hiroshima University
1986-1993
We improved the bispecific antibody platform that primarily engages natural killer (NK) cells to kill cancer through antibody-dependent cellular cytotoxicity (ADCC) by adding IL-15 as a crosslinker expands and self-sustains effector NK cell population. The overall goal was target B7-H3, an established marker predominantly expressed on minimally normal cells, prove it could in vitro inhibit tumor growth vivo. tri-specific engager (TriKETM) assembled DNA shuffling ligation using encoding...
In Brief BACKGROUND: Epidermal growth factor receptor (EGFR) is coactivated by the μ-opioid (MOR), expressed on non–small cell lung cancer (NSCLC) cells and human cancer. We hypothesized that clinically used opioid analgesics are MOR agonists coactivate EGFR, resulting in growth- survival-promoting signaling. METHODS: H2009, a adenocarcinoma NSCLC line, with constitutive EGFR phosphorylation, which showed increased expression of δ-opioid reverse transcriptase polymerase chain reaction....
Abstract Background : Using the Academy of Nutrition and Dietetics American Society for Parenteral Enteral (Academy/ASPEN) Consensus malnutrition definition, we estimated prevalence in a sample individuals with head neck cancer (HNC) compared it Patient‐Generated Subjective Global Assessment (PG‐SGA). We also investigated utility 50‐kHz phase angle (PA) 200‐kHz/5‐kHz impedance ratio (IR) to identify malnutrition. Materials Methods Nineteen (18 males, 1 female) scheduled undergo...
This pilot study evaluated nutrition status and health-related quality of life (HRQOL) outcomes among outpatients with head neck cancer (HNC). Data were collected from 19 patients (18 males, 1 female) during 3 time points: once before chemoradiotherapy (CRT) initiation months after CRT. Nutrition was using the Scored Patient-Generated Subjective Global Assessment (PG-SGA). Malnutrition defined as PG-SGA stage B (moderate/suspected malnutrition) or C (severely malnourished). HRQOL assessed...
New treatments are required to enhance current therapies for lung cancer. Mesothelin is a surface protein overexpressed in non-small cell cancer (NSCLC) that shows promise as an immunotherapeutic target phase I clinical trials. However, the immunosuppressive environment NSCLC may limit efficacy of these therapies. We applied time-of-flight mass cytometry examine state circulating mononuclear cells fourteen patients undergoing treatment unresectable Six had earlier stage (I-IVA) and eight...
8509 Background: The PACIFIC trial demonstrated that a year of consolidation PD-(L)1 inhibition following concurrent chemoradiation (CRT) for unresectable stage III NSCLC improves overall survival (OS). optimal duration IO therapy in this setting is undefined. Studies metastatic demonstrate combination PD-(L)1/CTLA-4 OS over chemotherapy alone. This evaluated the use Nivolumab (N) plus Ipilimumab (IPI) or N alone up to 6 months after CRT. Methods: randomized phase II, multicenter 105 pts...
Shoot primordia, which were able to propagate vegetatively with a very high rate and redifferentiate easily new plants, induced from shoot tips of Stevia rebaudiana Bertoni on Gamborg B5 medium containing 6-benzylaminopurine (BAP) alpha-naphthaleneacetic acid (NAA) under light. The propagation the primordia is rapid, they are highly stable in chromosome number karyotype. can at for long time without differentiation. At any time, readily developed into plantlets shoots roots within 2 or 3...
Abstract Background: Brassica vegetable consumption may confer a protective effect against cancer, possibly attributable to their glucosinolates. Glucobrassicin is predominant glucosinolate and the precursor of indole-3-carbinol (I3C), compound with anticancer effects. However, objective assessments I3C uptake from vegetables have not been successful. Methods: We conducted randomized, crossover trial test whether 3,3′-diindolylmethane (DIM, metabolite I3C) excreted in urine after raw...
Abstract Glucobrassicin in Brassica vegetables gives rise to indole-3-carbinol (I3C), a compound with potent anticancer effects preclinical models. We previously showed that the urinary metabolite 3,3′-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of vegetables. However, quantitative relationship glucobrassicin exposure DIM level is unclear. conducted clinical trial examine hypotheses range from reflected this effect plateaus. Forty-five subjects...
Although it is well established that human cytochrome P450 1 family enzymes are induced by cigarette smoking through activation of the Ah receptor, not known whether this leads to increased metabolic or detoxification carcinogenic polycyclic aromatic hydrocarbons (PAH), which present in smoke and general environment. We gave oral doses deuterated phenanthrene ([D10]Phe), a non-carcinogenic surrogate PAH such as benzo[a]pyrene, smokers (N = 170, 10 μg doses) non-smokers 57, dose)....
Tobacco smoking is the primary risk factor for lung cancer, driven by addictive nature of nicotine and indisputable carcinogenicity 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as well other compounds. The integration cancer chemoprevention with cessation one potential approach to reduce this mitigate mortality. Experimental data from our group suggest that kava, commonly consumed in South Pacific Islands a beverage promote relaxation, may enhancing NNK detoxification reducing...
Kava is regaining its popularity with detailed characterizations warranted. We developed an ultraperformance liquid chromatography high-resolution tandem mass spectrometry (UPLC-MS/MS) method for major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin and desmethoxyyangonin) excellent selectivity specificity. The has been validated different matrices following the Food Drug Administration guidance of analytical procedures methods validation. scope this demonstrated by...
Aim, materials & methods: Urinary cortisol profile has the potential as a diagnostic biomarker. We therefore developed stable-isotope dilution ultraperformance chromatography multistage MS-based method to quantify and 16 metabolites in human urines. Results conclusion: The LOD for its ranges from 0.02 5.81 pg/μl urine. inter- intraday variations were 3.7-12.9% 3.5-15.6%, respectively. Among analyzed, significant person-to-person heterogeneity was observed, demonstrating need comprehensive...
Human urinary DNA adducts may be useful surrogate biomarkers to estimate carcinogen exposure and activation, particularly if such are of high selectivity from a specific source. In this report, we provided evidence supporting tobacco use 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) being the dominant source for 3-methyladenine (3-mA), while nontobacco factors contribute significantly 7-methylguanine 1-methyladenine in urine. Upon confirmation human urine samples larger populations...
9010 Background: Consolidation PD-1/PD-L1 inhibition after chemoradiation (CRT) for unresectable stage III NSCLC improves overall survival. In IV NSCLC, the combination of nivolumab/ipilimumab improved survival compared to chemotherapy in patients with PD-L1 > 1% and performed favorably < 1%. The safety consolidation CRT has not been previously assessed. Methods: this randomized, multi-center, phase II study, a total 105 planned pts IIIA/IIIB will receive chemoradiation, then randomize...
Our earlier work demonstrated varying potency of dihydromethysticin (DHM) as the active kava phytochemical for prophylaxis tobacco carcinogen nicotine-derived nitrosamine ketone (NNK)-induced mouse lung carcinogenesis. Efficacy was dependent on timing DHM gavage ahead NNK insult. In addition to DNA adducts in tissues mitigated by a time-dependent manner, our vivo data strongly implicated existence damage-independent mechanism(s) NNK-induced carcinogenesis targeted fully exert its...
The extent of metabolism [D10]phenanthrene to [D(10)]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetradeuterophenanthrene ([D10]PheT) could be a biomarker human metabolic activation carcinogenic polycyclic aromatic hydrocarbons, leading identification smokers particularly susceptible lung cancer. longitudinal stability [D10]PheT was evaluated in 24 cigaret given 7-8 oral doses (10 µg) over 5.5 months. 6 h urine quantified after each dose. overall coefficient variation for subjects (mean ± S.D.) 27.4%...