A5044123136- Metal complexes synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Iron Metabolism and Disorders
- Nanoparticle-Based Drug Delivery
- Diet, Metabolism, and Disease
- RNA regulation and disease
- RNA modifications and cancer
- Pancreatic function and diabetes
- Ferrocene Chemistry and Applications
- Herpesvirus Infections and Treatments
- Marine Sponges and Natural Products
- Trace Elements in Health
- RNA Interference and Gene Delivery
- Hemophilia Treatment and Research
- Metabolism and Genetic Disorders
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Hair Growth and Disorders
- Cancer, Hypoxia, and Metabolism
- Skin and Cellular Biology Research
- Amino Acid Enzymes and Metabolism
- Neuroscience and Neuropharmacology Research
- Folate and B Vitamins Research
- Immune Response and Inflammation
- Metabolism, Diabetes, and Cancer
University of Naples Federico II
2015-2024
Istituto Nazionale Biostrutture e Biosistemi
2021
Naples Anesthesia & Physician Associates
2015
Istituto di Farmacologia Traslazionale
2015
Istituto di Chimica Biomolecolare
1983-2011
Shanghai Institute of Materia Medica
2011
Chinese Academy of Sciences
2011
Regione Campania
2005
Ceinge Biotecnologie Avanzate (Italy)
1996-2004
Duke University
1995
Abstract Alzheimer's disease is widely held to be associated with oxidative stress due, in part, the membrane action of β‐amyloid peptide aggregates . Here, we studied effect cannabidiol, a major non‐psychoactive component marijuana plant ( Cannabis sativa ) on peptide‐induced toxicity cultured rat pheocromocytoma PC12 cells. Following exposure cells (1 µg/mL), marked reduction cell survival was observed. This increased reactive oxygen species (ROS) production and lipid peroxidation, as well...
Two indole alkaloids, phidianidines A (1) and B (2), exhibiting an uncommon 1,2,4-oxadiazole ring linked to the system, have been isolated from marine opisthobranch mollusk Phidiana militaris. The structures of two metabolites elucidated by spectroscopic techniques. Phidianidines exhibit high cytotoxicity against tumor nontumor mammalian cell lines in vitro assays.
Based on the multifaceted molecular machinery that tightly controls iron cellular homeostasis, this review delves into its paradoxical, potentially dangerous role in biological systems, with a special focus double-edged sword correlations cancer. Indeed, though is vital micronutrient and required cofactor participating several essential cell functions, tendency to cause oxidative stress can be related both cancer risk activation of death pathways. In scenario, ferroptosis refers an...
This paper is dedicated to the memory of our wonderful colleague Professor Alfredo Colonna, who passed away same day its acceptance. Fatty liver accumulation, inflammatory process and insulin resistance appear be crucial in non-alcoholic fatty disease (NAFLD), nevertheless emerging findings pointed an important role also for iron overload. Here, we investigate molecular mechanisms hepatic metabolism onset steatosis understand whether impairment could early event injury. Rats were fed with...
Abstract According to WHO, breast cancer incidence is increasing so that the search for novel chemotherapeutic options nowadays an essential requirement fight neoplasm subtypes. By exploring new effective metal-based strategies, many ruthenium complexes have been recently proposed as antitumour drugs, showing ability impact on diverse cellular targets. In framework of different molecular pathways leading cell death in human models cancer, here we demonstrate autophagy involvement behind...
Cationic nanovectors loaded with Ru-based nucleolipids exert a high growth-inhibitory activity against human cancer cells (MCF-7 (A), WiDr (B), and HeLa (C)).
Many chemotherapeutic drugs cause nucleolar stress and p53-independent pathways mediating the response are emerging. Here, we demonstrate that ribosomal induced by Actinomycin D (Act D) is associated to up-regulation of protein L3 (rpL3) its accumulation as ribosome-free form in lung colon cancer cell lines devoid p53. Free rpL3 regulates p21 expression at transcriptional post-translational levels through a molecular mechanism involving extracellular-signal-regulated kinases1/2 (ERK1/2)...
An efficient drug delivery strategy is presented for novel anticancer amphiphilic ruthenium anionic complexes, based on the formation of stable nanoparticles with cationic lipid 1,2-dioleyl-3-trimethylammoniumpropane chloride (DOTAP). This aimed at ensuring high content within formulation, long half-life in physiological media, and enhanced cell uptake. in-depth microstructural characterization aggregates obtained mixing complex phospholipid carrier 50/50 molar ratio realized by combining a...
Looking for new metal-based anticancer treatments, in recent years many ruthenium complexes have been proposed as effective and safe potential drugs. In this context we recently developed a novel approach the vivo delivery of Ru(III) complexes, preparing stable ruthenium-based nucleolipidic nanoaggregates endowed with significant antiproliferative activity. Herein describe cellular response to our ruthenium-containing formulations selected models human breast cancer. By vitro bioscreens...
L-cysteine is currently recognized as a conditionally essential sulphur amino acid. Besides contributing to many biological pathways, cysteine key component of the keratin protein by its ability form disulfide bridges that confer strength and rigidity protein. In addition cysteine, iron represents another critical factor in regulating keratins expression epidermal tissues, well hair follicle growth maturation. By focusing on human keratinocytes, aim this study was evaluate effect...
Based on compelling preclinical evidence concerning the progress of our novel ruthenium-based metallotherapeutics, we are focusing research efforts challenging indications for treatment invasive neoplasms such as triple-negative breast cancer (TNBC). This malignancy mainly afflicts younger women, who black, or have a BRCA1 mutation. Because faster growing and spreading, TNBC differs from other cancers having fewer options worse prognosis, where existing therapies mostly ineffective,...
// Cynthia Licona 1, * , Marie-Elodie Spaety Antonelle Capuozzo 6 Moussa Ali 4 Rita Santamaria Olivier Armant 2 Francois Delalande 5 Alain Van Dorsselaer Sarah Cianferani John Spencer 3 Michel Pfeffer Georg Mellitzer 1 Christian Gaiddon INSERM 1113, Molecular Signaling of the Cell Stress Response and Pathology, Université de Strasbourg, Section Oncologie FMTS, France Karlsruhe Institute Technology (KIT), Toxicology Genetics (ITG), Germany Department Chemistry, School Life Sciences,...
Here we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy, molecular modelling, differential scanning calorimetry and prothrombin time assay, analogues of the thrombin binding aptamer (TBA) in which individual thymidines were replaced by 5-fluoro-2'-deoxyuridine residues. The whole data clearly indicate that all derivatives are able to fold a G-quadruplex structure very similar 'chair-like' conformation typical TBA. However, only ODNs TBA-F4:...
Several pharmaceutical products have been formulated over the past decades for treatment of male and female alopecia, pattern baldness, but relatively few metadata on their efficacy published. For these reasons, medical attention has recently focused discovery new safer remedies. Particularly, great interest attracted by oligomeric procyanidin bioactivity, able to promote hair epithelial cell growth as well induce anagen phase. Specifically, B2, a dimeric derivative extracted from apples,...
Degradation of nucleic acids in biological environments is the major drawback therapeutic use aptamers. Among approaches used to circumvent this negative aspect, introduction 3'-3' inversion polarity sites at sequence 3'-end has successfully been proposed. However, ends never exploited for G-quadruplex forming In communication we describe CD, UV, electrophoretic and biochemical investigations concerning thrombin binding aptamer analogues containing one or two inversions oligonucleotide ends....