A5010405974- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Machine Learning in Bioinformatics
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- HER2/EGFR in Cancer Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Bacteriophages and microbial interactions
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
University of Electronic Science and Technology of China
2016-2025
Second Affiliated Hospital of Zhejiang University
2025
Guiyang Medical University
2008-2025
Affiliated Hospital of Guizhou Medical University
2025
Zhejiang University
2019-2025
Hubei University of Medicine
2022-2025
Zhejiang Cancer Hospital
2022-2025
Sun Yat-sen University
2010-2025
Sun Yat-sen Memorial Hospital
2023-2025
Chengdu Neusoft University
2023-2025
A mouse model with a common human DMD mutation was created and corrected by CRISPR-Cas9 gene editing.
The successful prediction of protein subcellular localization directly from primary sequence is useful to function and drug discovery. In this paper, by using the concept pseudo amino acid composition (PseAAC), mycobacterial proteins are studied predicted support vector machine (SVM) increment diversity combined with modified Mahalanobis Discriminant (IDQD). results jackknife cross-validation for 450 nonredundant show that overall rates SVM IDQD 82.2% 79.1%, respectively. Compared other...
Summary A high‐amylose rice with 64.8% amylose content (AC) was developed by transgenic inhibition of two isoforms starch branching enzyme (SBE), SBEI and SBEIIb, in an indica cultivar. The expression SBEIIb completely inhibited the line, whereas granule‐bound synthase normal. Compared wild‐type rice, drastic reductions both SBEs increased apparent AC flour from 27.2% to 64.8%, resistant (RS) 0% 14.6% total dietary fibre (TDF) 6.8% 15.2%. Elevated proportion long unit chains amylopectin...
ABSTRACT Cytosolic DNA arising from intracellular pathogens is sensed by cyclic GMP-AMP synthase (cGAS) and triggers a powerful innate immune response. However, herpes simplex virus 1 (HSV-1), double-stranded virus, has developed multiple mechanisms to attenuate host antiviral machinery facilitate viral infection replication. In the present study, we found that HSV-1 tegument protein VP22 acts as an inhibitor of cGAS/stimulator interferon genes (cGAS/STING)-mediated production (IFN) its...
Self-complementary AAV-packaged CRISPR-Cas9 genome editing components rescue Duchenne muscular dystrophy.
Self-binding peptides (SBPs) represent those short peptide segments within monomeric proteins to fulfill their biological functions by dynamically binding to/unbinding from target domains in the same monomers. They are frequently found disordered or unstructured regions of that now known be not simply helices, loops, linkers and adopt a well-defined structure upon binding. In this study, we systematically explored structural basis, energy landscape, thermodynamic properties SBP-mediated...
Background: The location of proteins in a cell can provide important clues to their functions various biological processes. Thus, the application machine learning method prediction protein subcellular localization has become hotspot bioinformatics. As one key organelles, Golgi apparatus is charge storage, package, and distribution. Objective: identification will in-depth insights into functions. learning-based predicting been extensively explored. development sub-Golgi should be reviewed for...
By using state-of-the-art molecular dynamics to reconstruct the complete structural picture of self-binding peptides, a two-step binding mechanism was proposed, including fast, nonspecific diffusive phase and slow, specific organizational phase.
Mimotopes are peptides with affinities to given targets. They readily obtained through biopanning against combinatorial peptide libraries constructed by phage display and other technologies such as mRNA display, ribosome bacterial yeast display. have been used infer the protein interaction sites networks; they also ideal candidates for developing new diagnostics, therapeutics vaccines. However, valuable not collected in central data resources UniProt NCBI GenPept due their 'unnatural' short...
Skin can be infected by many types of microorganisms, most commonly gram‑positive strains Staphylococcus and Streptococcus spp. Treatment aureus (S. aureus) infections, particularly that methicillin resistant (MRSA), is a challenge in clinical practice. Ozone therapy has proven to one the strongest antiseptics against majority microorganisms involved skin infections. The purpose present study was evaluate microbicidal effects topical ozone on S. MRSA, determine efficacy patients with MRSA...
Drug-induced adverse reactions are a significant problem in healthcare worldwide and estimated to cost billions of dollars annually the United States. A portion such is observed strongly associate with certain human leukocyte antigen (HLA) alleles; one strongest associations HLA-B*1502 protein carbamazepine (CBZ)-induced Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) - odds ratio value can even be higher than thousand. The particularly strong association CBZ-induced SJS/TEN...
Three-dimensional (3D) hierarchical nanostructures have been considered as one of the most promising surface-enhanced Raman spectroscopy (SERS) substrates because ordered arrangement high-density hotspots along third dimension direction. Herein, we reported a unique 3D nanostructure for SERS detection based on silver nanoparticles (AgNPs) decorated zinc oxide/silicon (ZnO/Si) heterostructured nanomace arrays. They were prepared by two steps: (1) Si nanoneedles grafted onto ZnO nanorod arrays...
Surface-enhanced Raman scattering (SERS) has proven to be an effective technique for identifying and providing fingerprint structural information on various analytes in low concentration. However, this analytical been plagued by the ubiquitous presence of organic contaminants roughened SERS substrate surfaces, which not only often result poorer detection sensitivity but also significantly affect reproducibility accuracy analysis. Herein, we developed a clean, stable, recyclable...
Activation of PI3K/AKT signalling by PTEN loss significantly enhances chemoresistance in bladder cancer. This study aims to evaluate regulation and identify targets that could be used relieve chemoresistance. Expression YTHDC1, γ-H2AX were detected IHC assay. Cell Counting Kit-8 assay, colony formation assay tumour xenograft experiment evaluated cisplatin response. Flow cytometry comet estimated cell apoptosis, cycle distribution DNA repair capability. Quantitative real-time polymerase chain...
Antigen epitopes provide valuable information useful for disease prevention, diagnosis, and treatment. Recently, more databases focusing on different types of have become available. Conformational are an important form epitope formed by residues that sequentially discontinuous but close together in three-dimensional space. These implicit structural information, making them attractive both theoretical applied biomedical research. However, most existing focus linear rather than conformational...
Ca 2+ /calmodulin (CaM)-dependent phosphorylation of myosin regulatory light chain (RLC) in smooth muscle by kinase (MLCK) and dephosphorylation phosphatase (MLCP) are subject to modulatory cascades that influence the sensitivity RLC hence contraction intracellular concentration ([Ca ] i ). We designed a CaM-sensor MLCK containing fused two fluorescent proteins linked CaM-binding sequence measure activation vivo expressed it specifically mouse muscle. In phasic bladder muscle, there was...
As epitope mimics, mimotopes have been widely utilized in the study of prediction and development new diagnostics, therapeutics, vaccines. Screening random peptide libraries constructed with phage display or any other surface technologies provides an efficient convenient approach to acquire mimotopes. However, target-unrelated peptides creep into from time through binding contaminants components screening system. In this study, we present SAROTUP, a free web tool for scanning, reporting...