Abstract Background Vascular Endothelial Growth Factor (VEGF) is regulated by a number of different factors, but the mechanism(s) behind androgen-mediated regulation VEGF in prostate cancer are poorly understood. Results Three novel androgen receptor (AR) binding sites were discovered promoter and vivo AR to these was demonstrated chromatin immunoprecipitation. Mutation attenuated activation analog, R1881 cells. The transcription factors Sp1 shown form nuclear complex both bound core hormone...

// Ao Zhang 1, 2 , Masahiro Hitomi Noah Bar-Shain Zafardjan Dalimov 3 Leigh Ellis Kiran K. Velpula 4 Gail C. Fraizer 5 Robert G. Gourdie 6 Justin D. Lathia 2, 7 1 Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH, 44195, USA Department Cellular and Molecular Medicine, Research Institute, Clinic, Genitourinary Program, Pharmacology & Therapeutics, Roswell Park Cancer Buffalo NY, 14263, Biology Pharmacology, University Illinois Peoria, IL, 61656,...

The early growth response gene 1, EGR1, is an important transcriptional regulator and acts as the convergent point between a variety of extracellular stimuli activation target genes. Unlike other tumor types, prostate tumors express high levels EGR1 relative to normal tissues. However, mechanism regulation in cells unknown. As expression epidermal factor (EGF) signaling are frequently upregulated tumors, we tested hypothesis that EGF induces cancer cells. Using RT-PCR quantify transcripts,...

Abstract Background One key step in the development of prostate cancer (PCa) metastasis is loss E-cadherin expression associated with increased cellular motility and tumor invasion. This also required during normal embryogenesis similar transcriptional repressors have been identified both processes. We previously reported presence one such transcription factor, WT1 high Gleason grade tissues, its absence non-neoplastic or benign prostatic hyperplasia tissues. Results To better understand...

The Wilms' tumor gene, WT1, is expressed in few tissues, mainly the developing kidney, genitourinary system, and mesothelium, immature hematopoietic cells. To develop an understanding of role WT1 development tumorigenesis, we have identified transcriptional regulatory elements that function transient reporter gene constructs transfected into kidney cell lines. We found three transcription start sites essential promoter region by deletion analysis. a member GC-rich, TATA-less, CCAAT-less...

Abstract Background The prostate gland represents a multifaceted system in which epithelia and stroma have distinct physiological roles. To understand the interaction between glandular epithelia, it is essential to delineate gene expression profiles of these two tissue types cancer. Most studies compared tumor normal samples by performing global analysis using mixture cell populations. This report presents first study that examines patterns differential specific laser capture microdissection...

Chromosomal aneuploidy is associated with invasive bladder cancer and one of the genes implicated in these changes Aurora-A/STK15/BTAK, that localized on chromosome 20q13 encodes a centrosome-associated serine/threonine kinase. To better understand association between Aurora-A/STK15 expression, tumor clinical prognosis, we sought to determine whether overexpression cultured urothelial cells facilitated chromosomal instability. Using immunofluorescence staining, Northern Western blot...

Expression of the Wilms' tumor gene WT1 is tightly regulated throughout development. In contrast, promoter promiscuous, functioning in all cell lines tested. We have cloned a transcriptional silencer that involved regulation gene. The located third intron gene, approximately 12 kilobases from promoter, and functions to repress transcription non-renal origin. 460-base pair region unusual it contains full-length Alu repeat. also an enhancer like-element 1.3 upstream promoter.

The Wilms' tumor gene, WT1, encodes a zinc finger transcription factor that can repress of number genes. WT1 mRNA undergoes alternative splicing at two locations, yielding four different species and protein products. One splice alters the region resulting in addition three amino acids, Lys-Thr-Ser (KTS), between fingers 3 4, altering binding to DNA. Here, we show with without KTS tripeptide from human full-length promoter. Repression by has been shown require sites. We examined repression...

The Wilms' tumor gene (WT1) is an essential for kidney and gonadal development, although how WT1 expression induced in these tissues not known. One transcription factor likely to play a role this regulation PAX 8. co-expression of 8 during development tumors with epithelium predominant histology suggested possible interaction, indeed, we identified potential core PAX-binding sites the promoter. Endogenous plays important activation WT1promoter, since promoter activity much stronger cells...

The Wilms' tumor 1 gene (WT1) encodes a zinc-finger transcription factor which is expressed in tissue-specific manner. Our studies indicate that addition to the promoter, other regulatory elements are required for expression of this gene. A 258-base pair hematopoietic specific enhancer intron 3 WT1 increased transcriptional activity promoter by 8-10-fold K562 and HL60 cells. Sequence analysis revealed both GATA c-Myb motif fragment. Mutation decreased 60% Electrophoretic mobility shift...

Abstract As S-phase checkpoints play critical roles in maintaining genomic integrity and replicating the human genome correctly, understanding molecular mechanism by which they regulate therapeutic response is of great interest. Previously, we reported that cytotoxic effect a zinc-bound form Apo2 ligand/tumor necrosis factor–related apoptosis-inducing ligand (Apo2L/TRAIL), currently evaluated clinical trials, combination with low-dose CPT-11, induces apoptosis C4-2 prostate cancer cells...

Abstract Background Gene expression analyses have led to a better understanding of growth control prostate cancer cells. We and others identified the presence several zinc finger transcription factors in neoplastic prostate, suggesting potential role for these genes regulation transcriptome. One (TFs) epithelial cells was Wilms tumor gene ( WT1 ). To rapidly identify coordinately expressed that may be regulated by WT1, we used an silico approach. Results Evolutionary conserved factor binding...

Abstract Signal intensity in fluorescence microscopy is often measured relative to arbitrary standards. We propose a calibration method based on solution of the same fluorophore, whose binding cells needs be quantified. The utilizes low sensitivity object distance wide‐field imaging uniform materials. Liquid layers slowly varying depth were prepared by immersing spherical lens into drop fluorophore placed slide. Flatfield‐corrected images contact and surrounding areas showed linear...

The primary form of therapy for prostate cancer is androgen ablation resulting in apoptosis and expression apoptotic genes (i.e. par-4). Prostate cells that survive express pro-survival bcl-2) permitting these independent (AI) to overcome signals proliferate the absence normal growth signals. To disrupt tumor progression AI, we expressed suppressor gene, WT1 LNCaP cells. transcription factor modulates activity several control par-4, bcl-2 AR) vitro. provide insight into potential mechanisms...

The expression of a hematopoietic proteoglycan core protein (HpPG) gene is up-regulated during the early stages myeloblast differentiation at time point coinciding with beginning granule genesis (Stellrecht, C. M., Mars, W. Miwa, H., Beran, and Saunders, G. F. (1991) Differentiation 48, 127-135). mechanism this up-regulatory event was investigated by analyzing regulation HpPG pluripotent cell line, K562. level in these cells approximately 10-fold upon 12-O-tetradecanoylphorbol-13-acetate...