A5026631806- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Seed and Plant Biochemistry
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Enzyme Structure and Function
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Marine Sponges and Natural Products
- Plant-Microbe Interactions and Immunity
- Advanced Fluorescence Microscopy Techniques
- Plant nutrient uptake and metabolism
- Crystallization and Solubility Studies
- Bacteriophages and microbial interactions
- X-ray Diffraction in Crystallography
- Plant Reproductive Biology
- Potato Plant Research
- Polysaccharides and Plant Cell Walls
- Nanocluster Synthesis and Applications
Hainan University
2017-2025
Henan University
2019-2025
Northwest A&F University
2014-2025
Guizhou Academy of Agricultural Sciences
2022-2025
Guizhou Electric Power Design and Research Institute
2025
Jiangsu Province Hospital
2025
China Institute of Water Resources and Hydropower Research
2024
Sanya University
2024
Beijing Institute of Nanoenergy and Nanosystems
2024
Chinese Academy of Sciences
2024
Monovalent salts are generally believed to stabilize DNA duplex by weakening inter-strand electrostatic repulsion. Unexpectedly, our force-induced hairpin unzipping experiments and thermal melting show that LiCl, NaCl, KCl, RbCl, CsCl at concentrations beyond ~1 M destabilize DNA, RNA, RNA-DNA duplexes. The two types of yield different changes in free energy during melting, while the results high concentration monovalent duplexes common. effects these ions similar but also have noticeable...
Mutations in the RecQ DNA helicase gene BLM give rise to Bloom's syndrome, which is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition. highly active binding unwinding G-quadruplexes (G4s), are physiological targets for BLM, as revealed genome-wide characterizations of expression cells from BS patients. With smFRET assays, we studied molecular mechanism BLM-catalyzed G4 unfolding showed that ATP required unfolding. Surprisingly, depending on...
G-quadruplex (G4) can be formed by G-rich DNA sequences that are widely distributed throughout the human genome. Although G-triplex and G-hairpin have been proposed as G4 folding intermediates, their formation still requires further investigation experiments. Here, we employed single-molecule FRET to characterize dynamics of from telomeric sequence. First, observed four states during initially assigned anti-parallel G4, G-triplex, unfolded ssDNA. Then constructed putative intra-strand...
Recent advances in G-quadruplex (G4) studies have confirmed that G4 structures exist living cells and may detrimental effects on various DNA transactions. How helicases resolve G4, however, has just begun to be studied remains largely unknown. In the present paper, we use single-molecule fluorescence assays probe Pif1-catalysed unfolding of a construct resembling an ongoing synthesis lagging strand stalled by G4. Strikingly, Pif1 unfolds then halts at ss/dsDNA junction, followed rapid...
Abstract Water transportation to developing tissues relies on the structure and function of plant xylem cells. Plant microtubules govern direction cellulose microfibrils guide secondary cell wall formation morphogenesis. However, relevance microtubule-determined thickening patterns in hydraulic conductivity remains unclear. In present study, we identified a maize (Zea mays) semi-dominant mutant, designated drought-overly-sensitive1 (ZmDos1), upper leaves which wilted even when exposed...
The Saccharomyces cerevisiae Pif1 protein (ScPif1p) is the prototypical member of family DNA helicases. ScPif1p involved in maintenance mitochondrial, ribosomal and telomeric suppresses genome instability at G-quadruplex motifs. Here, we report crystal structures a truncated (ScPif1p237-780) complex with different ssDNAs. Our results have revealed that yeast-specific insertion domain protruding from 2B folds as bundle bearing an α-helix, α16. α16 helix regulates helicase activities through...
As a key dsDNA recognition receptor, cyclic guanosine monophosphate (GMP)-AMP synthase (cGAS) plays vital role in innate immune responses. Activated cGAS, by sensing DNA, catalyzes the synthesis of secondary messenger GMP-AMP (cGAMP), which subsequently activates downstream signaling to induce production interferons and inflammatory cytokines. Here, we report Zyg-11 family member B (ZYG11B) as potent amplifier cGAS-mediated Knockdown ZYG11B impairs cGAMP subsequent transcription interferon...
The unique dumbbell-shape of grass guard cells (GCs) is controlled by their cell walls which enable rapid responses to the environment. molecular mechanisms regulating synthesis and assembly GC are as yet unknown. Here we have identified BZU3, a maize gene encoding UDP-glucose 4-epimerase that regulates supply during wall synthesis. BZU3 mutation leads significant decreases in cellular levels. Immunofluorescence intensities reporting levels cellulose mixed-linkage glucans reduced GCs,...
The trihelix family of transcription factors plays essential roles in the growth, development, and abiotic stress response plants. Although several studies have been performed on gene dicots monocots, this is yet to be studied Chenopodium quinoa (quinoa).In study, 47 C. (CqTH) genes were genome. Phylogenetic analysis CqTH from Arabidopsis thaliana Beta vulgaris revealed that clustered into five subfamilies: SIP1, GTγ, GT1, GT2, SH4. Additionally, synteny located 17 chromosomes, with...
ABSTRACT Balancing land development and ecological protection poses significant challenges for sustainable in arid semi‐arid regions like Inner Mongolia. This study uses the integrated valuation of ecosystem services (ESs) trade‐offs model patch‐generated land‐use simulation to analyze impacts land‐use/cover (LULC) changes on ESs from 2005 2020 simulate scenarios 2035. The results show that (1) governance efforts improved comprehensive index (CEI), habitat quality, soil conservation capacity...
To study the response characteristics of long-term mulching measures on soil enzyme activity and limitation microbial nutrients in Loess Plateau Region, adopted a random block design setup based field positioning experiment conducted 2008. Four types inter-field management were established, including conventional no-mulch （NM）, straw mulch （SM）, （PM）, ridge （RPM）. The changes extracellular activities, their ecological stoichiometry influencing factors under different cover measures,...
Abstract Background The development of highly efficient and environmentally friendly plant virus inhibitors with novel structures mostly depends on the discovery new targets. G‐quadruplexes are non‐classical high‐level that formed by guanine‐rich nucleic acid sequences, which have significant biological functions. Small molecules target in genomes animal viruses been shown to inhibit viral proliferation. However, structure function G‐quadruplex genome rarely reported. Investigating...
Abstract Werner syndrome is caused by mutations in the WRN gene encoding helicase. A knowledge of helicase’s DNA unwinding mechanism vitro helpful for predicting its behaviors vivo , and then understanding their biological functions. In present study, deeply WRN, we comprehensively characterized properties chicken helicase core details, taking advantages single-molecule fluorescence resonance energy transfer (smFRET) method. We showed that exhibits repetitive translocation on different...
Photoluminescence (PL) metal nanoclusters (NCs) have attracted extensive attention due to their excellent physicochemical properties, good biocompatibility, and broad application prospects. However, developing water-soluble PL NCs with a high quantum yield (QY) stability for visual drug delivery remains great challenge. Herein, we synthesized ultrabright l-Arg-ATT-Au/Ag (Au/Ag NCs) QY as 73% photostability by heteroatom doping surface rigidization in aqueous solution. The as-prepared Au/Ag...
Rapid land-use changes have significantly changed the occurrence of heavy metals (HMs) in tropical watershed systems. However, influence patterns on spatial and temporal distribution HMs river systems remains poorly understood. This study aims to explore relationship between types HM pollution China’s largest watershed, Nandu River. Eight (Cd, Pb, Cr, Cu, Zn, As, Hg, Sb) surface water were monitored across river, estuary, nearshore zones during wet dry seasons. Our findings show a higher...
Telomeres are specific nucleoprotein structures that located at the ends of linear eukaryotic chromosomes and play crucial roles in genomic stability. Telomere DNA consists simple repeats a short G-rich sequence: TTAGGG mammals TTTAGGG most plants. In recent years, mammalian telomeric have been shown to form G-quadruplex (G4) structures, which for modulating telomere functions. Surprisingly, even though plant telomeres essential growth, development, environmental adaptions, only few reports...
I-motifs are four-strand noncanonical secondary structures formed by cytosine (C)-rich sequences in living cells. The structural dynamics of i-motifs play essential roles many cellular processes, such as telomerase inhibition, DNA replication, and transcriptional regulation. In cells, the i-motif can be modulated interaction poly(C)-binding proteins (PCBPs), is closely related to human health, through modulating transcription oncogenes telomere stability. Therefore, mechanisms how PCBPs...
Anthracnose disease caused by Colletotrichum gloeosporioides is one of the devastating diseases yams (Dioscorea sp.) worldwide. In this study, we aimed to isolate endophytic actinobacteria from yam plants and evaluate their potential for control anthracnose based on bioassays genomic analyses. A total 116 actinomycete strains were isolated surface-sterilized tissues a orchard in Hainan Province, China. total, 23 isolates showed antagonistic activity against C. gloeosporioides. An...
Guanine (G) quadruplexes (G4s) can be formed by G-rich sequences when stabilized the binding of cations (typically K+ or Na+) and play an essential role in replication, recombination, transcription, telomere maintenance. Understanding G4 folding process is crucial for determining their cellular functions. However, G4-K+ interactions pathways are still not well understood. By using human telomeric (hTG4) as example, two states corresponding to hTG4 were distinguished clearly fitted precisely....
Alternative DNA structures that deviate from B-form double-stranded such as G-quadruplex (G4) can be formed by G-rich sequences are widely distributed throughout the human genome. We have previously shown Pif1p not only unfolds G4, but also unwinds downstream duplex in a G4-stimulated manner. In present study, we further characterized unwinding phenomenon means of single-molecule fluorescence resonance energy transfer. It was found did unwind partial immediately after unfolding upstream G4...