A5089746868- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Chromatin Remodeling and Cancer
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Advanced Electron Microscopy Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Exercise and Physiological Responses
- Mesenchymal stem cell research
- Advanced Fluorescence Microscopy Techniques
- Plant Molecular Biology Research
- Mechanisms of cancer metastasis
Sylvester Comprehensive Cancer Center
2023-2024
University of Miami
2023-2024
Hebrew University of Jerusalem
2013-2020
Nanyang Technological University
2012-2014
Growth factors, such as myostatin (Mstn), play an important role in regulating post-natal myogenesis. In fact, loss of Mstn has been shown to result increased muscle growth through enhanced satellite cell functionality; while elevated levels dramatic skeletal wasting a mechanism involving reduced protein synthesis and ubiquitin-mediated degradation. Here we show that miR-27a/b plays feed back auto-regulation thus regulation Sequence analysis 3' UTR showed single highly conserved binding site...
Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and self-renew. These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks hyperdynamic binding of structural proteins. Recently, several chromatin-related proteins been shown regulate ESC pluripotency and/or differentiation, yet role major heterochromatin in is unknown. Here we identify Heterochromatin Protein 1β (HP1β) as...
Repressor element-1 silencing transcription factor (REST) is required for the formation of mature neurons. REST dysregulation underlies a key mechanism neurodegeneration associated with neurological disorders. However, mechanisms leading to alterations REST-mediated neurogenesis genes are not known. Here, we show that BRCA1 Associated ATM Activator 1 (BRAT1), gene linked neurodegenerative diseases, activation REST-responsive during neuronal differentiation. We find INTS11 and INTS9 subunits...
In BriefAlajem et al. develop an assay that indicates differential association of SMARCD1 with chromatin in embryonic stem cells (ESCs) and earlydifferentiating cells.SMARCD1 is associated bivalent genes ESCs, regulates H3K4me3/H3K27me3 distribution, binds the pluripotency factor Klf4.
The transcriptional landscape in embryonic stem cells (ESCs) and during ESC differentiation has received considerable attention, albeit mostly confined to the polyadenylated fraction of RNA, whereas non-polyadenylated (NPA) remained largely unexplored. Notwithstanding, NPA RNA super-family every potential participate regulation pluripotency cell fate. We conducted a comprehensive analysis ESCs using combination whole-genome tiling arrays deep sequencing technologies. In addition identifying...
Classically, peroxisome proliferator-activated receptor β/δ (PPARβ/δ) function was thought to be restricted enhancing adipocyte differentiation and development of adipose-like cells from other lineages. However, recent studies have revealed a critical role for PPARβ/δ during skeletal muscle growth regeneration. Although has been implicated in regulating myogenesis, little is presently known about the and, that matter, mechanism(s) action postnatal myogenesis. Here we report first time, using...
Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used study differentiation, epigenetic regulation, lineage choices, more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse ESCs, we generated library over 200 endogenously tagged fluorescent fusion proteins present several proof-of-concept applications this library. We show the utility track in living cells; screen for pluripotency-related factors; identify...
Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed rapid isoform shift early differentiation from the predominant ESCs, SETα, to primary differentiated cells, SETβ, through alternative promoters. SETα is selectively...
Integrator is a multi-subunits protein complex involved in regulation of gene expression. Several subunits have been found to be mutated human neurodevelopmental disorders, suggesting key role for the development nervous system. BRAT1 similarly linked with neurodegenerative diseases and disorders such as rigidity multifocal-seizure syndrome. Here, we show that INTS11 INTS9 interact form trimeric HEK293T cells well pluripotent embryonal carcinoma cell line (NT2). We find depletion disrupts...
The multifunctional histone chaperone, SET, is essential for embryonic development in the mouse. Previously, we identified SET as a factor that rapidly downregulated during stem cell (ESC) differentiation, suggesting possible role maintenance of pluripotency. Here, explore SET's function early differentiation. Using immunoprecipitation coupled with protein quantitation by LC-MS/MS, uncover factors and complexes, including P53 β-catenin, which regulates lineage specification. Knockdown...