A5083300368- Pharmacological Effects and Toxicity Studies
- Epilepsy research and treatment
- Parkinson's Disease Mechanisms and Treatments
- Forensic Toxicology and Drug Analysis
- Neurological disorders and treatments
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Cannabis and Cannabinoid Research
- Alcohol Consumption and Health Effects
- Biosimilars and Bioanalytical Methods
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
- Trypanosoma species research and implications
- Psychedelics and Drug Studies
- Sphingolipid Metabolism and Signaling
- Antibiotics Pharmacokinetics and Efficacy
- Restless Legs Syndrome Research
- Cancer Treatment and Pharmacology
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Intracranial Aneurysms: Treatment and Complications
- Traumatic Brain Injury and Neurovascular Disturbances
- Atmospheric chemistry and aerosols
Cairo University
2025
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2024
Istituto delle Scienze Neurologiche di Bologna
2012-2024
University of Bologna
2008-2024
Helwan University
2020
Higher Institute of Engineering
2020
Institute of Neurological Sciences
2013-2018
Lacosamide (LCM) is one of the newer antiepileptic drugs (AEDs) licensed as add-on treatment for partial epilepsy. Data on LCM pharmacokinetics and interactions are limited partly contradictory. The purpose this study was to assess effect concomitant AED therapy steady state plasma concentrations in a population patients with epilepsy.Steady were assessed cohort 75 consecutive epilepsy referred Laboratory Clinical Neuropharmacology therapeutic monitoring over 16 months. Plasma measured by...
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a fatal, recessive disease caused by mutations in the gene encoding thymidine phosphorylase, leading to reduced enzymatic activity, toxic nucleoside accumulation, and secondary mitochondrial DNA damage. Thymidine phosphorylase replacement has been achieved allogeneic hematopoietic stem cell transplantation, procedure hampered high mortality. Based on expression liver, 25-year-old severely affected patient underwent liver...
Introduction: In the dynamic universe of new psychoactive substances (NPS), identification multiple and chemically diverse compounds remains a challenge for forensic laboratories.Since hair analysis represents gold-standard to assess prevalence NPS, which are commonly detected together with classical drugs abuse (DoA), our study aimed at developing wide-screen method detect quantify 127 NPS 15 DoA on hair.Materials methods: A multi-analyte ultra-high performance liquid chromatography mass...
ADCA-DN and HSN-IE are rare neurodegenerative syndromes caused by dominant mutations in the replication foci targeting sequence (RFTS) of DNA methyltransferase 1 (DNMT1) gene. Both phenotypes resemble mitochondrial disorders, dysfunction was first observed ADCA-DN. To explore involvement, we studied effects DNMT1 fibroblasts from four two patients. We documented impaired activity purified mutant proteins, which results increased amount. demonstrated that is not localized within mitochondria,...
Summary We prospectively examined the effect of antiepileptic ( AED ) cotherapy on steady state plasma concentrations perampanel PMP in epileptic patients. classified s as strong enzyme inducers (carbamazepine, phenobarbital, phenytoin, oxcarbazepine), not inducers/not inhibitors (levetiracetam, lamotrigine, topiramate, rufinamide, lacosamide, zonisamide, clobazam), and (valproic acid [ VPA ]). The main outcome was comparison concentration to weight‐adjusted dose ratio (C/D; [μg/mL]/mg kg −1...
We report a rapid and sensitive LC-MS/MS method that allows the simultaneous detection of 68 commonly prescribed antidepressants, benzodiazepines, neuroleptics, metabolites in whole blood with small sample volume after protein precipitation. The was also tested on post-mortem from 85 forensic autopsies. Three sets commercial serum calibrators containing mix prescription drugs increasing concentration were spiked red cells (RBC) to obtain 6 (3 "serum calibrators" 3 "blood calibrators")....
Abstract People diagnosed with substance use disorder (SUD) might represent a high‐risk subpopulation for New Psychoactive Drugs (NPS) consumption, and hair analysis offers unique perspective to assess drug prevalence in this population. The present study aims the of NPS their co‐consumption traditional drugs abuse (DoA) individuals SUD. Hair samples from patients under care at addiction treatment service Bologna, Italy, SUD, were collected during 2023 analyzed by ultra‐high‐performance...
We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using mobile phase sodium acetate 0.03 M pH 3.7 acetonitrile (40/60, v/v), at flow rate 0.8 mL/min. Total time each run 5 min. Sample preparation (250 µL) involved only one precipitation step by spiked mirtazapine as internal...
Molecularly imprinted polymers (MIPs) are synthetic receptors designed to selectively bind specific molecules, mimicking natural antibody–antigen interactions. Produced through polymerization around a target molecule (template), MIPs create imprints that confer high specificity and binding affinity upon template removal. Initially developed in the 1970s with organic polymers, now play critical roles separation sciences, catalysis, drug delivery, sensor technology. In forensic science, offer...
Introduction Epilepsy is a chronic condition requiring consistent follow-up aimed at seizure control, and monitoring of anti-seizure medication (ASM) levels side effects. Telemedicine (TM) offers invaluable support to patient follow-up, guaranteeing the prompt availability team experts for persons with epilepsy (PWE) widely distributed across country. Although many health institutions have endorsed use TM, robust data on effectiveness, safety costs TM applied are lacking. TELEmedicine...
Objectives The aim was to evaluate plasma and cerebrospinal fluid (CSF) nimodipine concentrations in patients with aneurysmal subarachnoid hemorrhage their correlation clinical outcome. Methods Nimodipine infusion started at 1 mg/h increased up 2 continued 21 days surviving patients. Arterial CSF samples were collected least after 24 hours of stable dosing. Delayed cerebral ischemia vasospasm documented by new neurological deficits neuroimaging. outcome assessed 9 months the modified Rankin...
Objectives We aimed to assess the potential relationship between intrasubject 9-tetrahydrocannabinol/cannabidiol (THC/CBD) oromucosal spray plasma profiles and clinical effects elicited by subacute dosing in chronically treated patients with multiple sclerosis (MS). Methods The study design was pilot, single center, open, prospective. were challenged a morning test dose of 2 THC/CBD sprays at 15-minute interval. Venous blood samples collected before first administration every 30 minutes...
Levodopa-carbidopa intestinal gel (LCIG) treatment has shown variable effect on dyskinesia in Parkinson's disease (PD).To identify PD patients who are likely to have troublesome under LCIG and describe the pharmacokinetic-dynamic profile phenomenology of those patients.PD were assessed for clinical therapeutic variables, before (T0) at last outpatient visit (T1). Sub-groups with without "troublesome dyskinesia" (UPDRS IV, item 33 ≥2), matched duration, underwent a assessment.We included 53...