In this paper, a kind of green triboelectric nano-generator based on natural degradable cellulose is proposed. Different kinds regenerated composite layers are prepared by blending doping method, and then assembled with poly(tetrafluoroethylene) (PTFE) thin films to form tribioelectric nanogenerator (TENG). The results show that the open circuit output voltage short current using pure membrane 7.925 V 1.095 μA. After adding certain amount polyamide (PA6)/polyvinylidene fluoride (PVDF)/barium...

Salidroside and its aglycone p-tyrosol are two major phenols in the genus Rhodiola have been confirmed to possess various pharmacological properties. In our present study, was identified as deglycosylation metabolite of salidroside after intravenous (i.v.) administration rats at a dose 50 mg/kg, but not detectable intragastric gavage (i.g.) through HPLC-photodiode array detection (PDA) liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Next, an accurate precise LC-MS/MS...

Small-molecule carboxyl methyltransferases (CbMTs) constitute a small proportion of the reported methyltransferases, but they have received extensive attention due to their important physiological functions. Most small-molecule CbMTs isolated date originate from plants and are members SABATH family. In this study, we identified type CbMT (OPCMT) group Mycobacteria , which has distinct catalytic mechanism methyltransferases. The enzyme contains large hydrophobic substrate-binding pocket (~400...

Base editors are double-strand-break (DSB)-free genome editing tools and have been widely used in diverse living systems. However, it is reported that these can cause substantial off-target editing.

In the present work, salidroside metabolite profile in rat urine was investigated, and subsequently metabolic pathways of were proposed. After administrations at an oral dose 100 or 500 mg/kg, samples collected pretreated with methanol to precipitate proteins. The analyzed by Acquity ultraperformance liquid chromatography (UPLC) coupled HSS T3 column detected quadrupole time-of-flight mass spectrometry (Q-TOF-MS) high-performance hybrid triple-quadrupole linear ion trap (HPLC/Q-trap-MS). A...

Glucosinolates (GLs) are plant secondary metabolites that may act against different types of cancers. Broccoli (Brassica oleracea var. italica) is rich in GLs which makes it an excellent source these nutraceuticals. The composition and concentration vary among broccoli cultivars throughout the developmental stages plant. To obtain GL profiles broccoli, compositions contents four early (seeds, 3-day sprouts, 11-day 17-day seedlings) were determined for nine this study. A total 12 including 9...

A viral vector is a genetically modified produced by genetic engineering. As pathogenic genes in the virus are completely or largely eliminated, it safe to be widely used multidisciplinary research fields for expressing genes, such as neuroscience, metabolism, oncology and so on. Neuroscience psychiatry most closely related disciplines either basic clinical research, but application of vectors neuropsychiatry has not received much attention been accepted.This article will focus on...

Context: Salidroside and its metabolite p-tyrosol are two major phenols in the genus Rhodiola L. (Crassulaceae). They have been confirmed to possess various pharmacological properties used for prophylaxis therapeutics of many diseases. Several analytical methods developed determination compounds plant materials biological plasma matrices. However, these not optimal samples containing complex organic interferences, such as liver brain tissues.Objective: This study aimed further develop...

Abstract Lupeol, a phytosterol and triterpene, is widely found in edible fruits vegetables, has been reported to exhibit spectrum of pharmacological activities against various disease conditions. In the present study, derivative generated by reaction lupeol with p ‐toluenesulfonyl isocyanate was ionizable fragmentable negative mode electrospray ionization/tandem mass spectrometry. Based on this simple chemical derivatization, liquid chromatography–electrospray spectrometry method developed...

A type III polyketide synthase (SfuPKS1) from the edible seaweed Sargassum fusiforme was molecularly cloned and biochemically characterized. The recombinant SfuPKS1 catalyzed condensation of fatty acyl-CoA with two or three malonyl-CoA using lactone-type intramolecular cyclization to produce tri- and/or tetraketides. Moreover, it can also utilize phenylpropanoyl-CoA synthesize phloroglucinol derivatives through Claisen-type cyclization, exhibiting broad substrate catalysis specificity....

Dichloroacetate (DCA) has potential for treating mitochondrial disorders and cancer by activating the pyruvate dehydrogenase complex. Repeated dosing of DCA results in reduced drug clearance due to inactivation glutathione transferase ζ1 (GSTZ1), its metabolizing enzyme. We investigated time-course GSTZ1 hepatic cytosol mitochondria after one oral dose 100 mg/kg female Sprague-Dawley rats aged 4 weeks (young) 52 (adult) as models children adults, respectively. activity with both an...

Two new ansamycin derivatives, damavaricin H (1) and protostreptovaricin VI (2) were isolated from the Streptomyces spectabilis CCTCC M2017417 derived mutants of ΔstvP5 ΔstvA2, respectively. The structures 1 2 established by analysis HRESIMS as well 1D 2D NMR datasets. minimum inhibitory concentration (MIC) results showed that compounds possessed corresponding anti-MRSA bioactivities 4 ∼ 8 μg/ml 16 μg/ml, which confirmed structure-activity relationships streptovaricins reported previously...

Glucosinolates (GSLs), crucial secondary metabolites in cruciferous vegetables, hydrolyze upon consumption or mechanical damage, forming bioactive compounds with anti-cancer properties, such as glucoraphanin (GRA). Among broccoli stands out for its high GSL content, which varies significantly among different genotypes. This study aimed to characterize and quantify glucosinolate using LC-HRMS2 UPLC. We identified thirteen GSLs 191 genotypes, including seven aliphatic, five indole, one...

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metabolites were 76.9%- 102.8%, the precision ranged from 5.92% to 10. 19%, limits of quantification 0. 001 01 mg/L, and linear correlation coefficients 998 2-0. 999 9 in range -1. 00 mg/L. This method was applied analysis Chinese cabbages, hot peppers, tomatoes onions markets. can be rapid detection organophosphorus pesticides their vegetables. 76.9%-102.8%, 10.19%, 0.001 0.01 0.9982-0.9999 0.01-1.00

Gastroesophageal reflux disease (GERD) is a commonly encountered condition in children, which at times causes respiratory distress, such as asthmatic symptoms, and results serious morbidity even mortality. The complexity sometimes so obscure, that it can cause paradoxical diagnoses treatment. Here we present two cases of children with difficult-to-treat were eventually found to be related GERD. treated anti-reflux procedures both became symptom free. Literature was also reviewed shed light...