We report an integrated microfluidic device for fine-scale manipulation and confinement of micro- nanoscale particles in free-solution. Using this device, single are trapped a stagnation point flow at the junction two intersecting microchannels. The hydrodynamic trap is based on active control fluid using on-chip valve monolithic PDMS-based device. In work, we characterize design parameters enabling precise position efficient performance. microfluidic-based facilitates particle trapping sole...

Trapping and manipulation of microscale nanoscale particles is demonstrated using the sole action hydrodynamic forces. We developed an automated particle trap based on a stagnation point flow generated in microfluidic device. The enables confinement single low viscosity (1–10 cP) aqueous solution. Using this method, we trapped (100 nm–15 μm) for long time scales (minutes to hours). demonstrate within 1 μm center, corresponding stiffness ∼10−5–10−4 pN/nm.

High precision control of micro- and nanoscale objects in aqueous media is an essential technology for nanoscience engineering. Existing methods particle trapping primarily depend on optical, magnetic, electrokinetic, acoustic fields. In this work, we report a new hydrodynamic flow based approach that allows fine-scale manipulation positioning single particles using automated fluid flow. As proof-of-concept, demonstrate two-dimensional (2D) 500 nm 2.2 μm diameter with as small 180 during...

Traditional cultivation approaches in microbiology are labor-intensive, low-throughput, and yield biased sampling of environmental microbes due to ecological evolutionary factors. New strategies needed for ample representation rare taxa slow-growers that often outcompeted by fast-growers experiments. Here we describe a microfluidic platform anaerobically isolates cultivates microbial cells millions picoliter droplets automatically sorts them based on colony density enhance slow-growing...

Lasing from spherical microdroplets ejected into a liquid medium with lower refractive index is observed in microchannel. A microfabricated device that combines droplet production and excitation/detection has been utilized. Droplets of 50 microm diameter containing fluorescent dye were first detected then excited through multimode fibers after their at T-junction. Images show intense lasing emission around the rim. Spectra droplets exhibit morphology-dependent resonances are redshifted...

The single cell microbioreactor allows for precise and rapid control over the growth environment cells cultured in free solution, thereby facilitating direct analysis of intracellular dynamics.

An increase in microsensor deflection with an blood viscosity during coagulation.

Recent advances in fluorescence microscopy have enabled high-resolution imaging and tracking of single proteins biomolecules cells. To achieve high spatial resolutions the nanometer range, bright photostable fluorescent probes are critically required. From this view, there is a strong need for development advanced with molecular-scale dimensions imaging. Polymer-based dendrimer nanoconjugates hold potential to serve as versatile due an intrinsic capacity tailored spectral properties such...

A microfluidic-based directed assembly strategy is employed to form highly aligned supramolecular structures. Formation of synthetic oligopeptide nanostructures accomplished using planar extensional flow, which induces alignment underlying material suprastructures. Fluidic-directed structures allows for unprecedented manipulation at the nano- and mesoscales, has potential provide rapid efficient control functional properties.

Multiplexed diagnostic testing has the potential to dramatically improve quality of healthcare. Simultaneous measurement health indicators and/or disease markers reduces turnaround time and analysis cost speeds up decision making process for diagnosis treatment. At present, however, most tests only provide information on a single indicator or marker. Development efficient capable parallel screening infectious could significantly advance clinical in both developed developing parts world....

We developed a microfluidic analogue of the classic Wheatstone bridge circuit for automated, real-time sampling solutions in flow-through device format. demonstrate precise control flow rate and direction "bridge" microchannel using an on-chip membrane valve, which functions as integrated "variable resistor". implement automated feedback mechanism order to dynamically adjust valve opening, thereby manipulating pressure drop across precisely controlling fluid channel. At critical channel can...

The ability to confine and manipulate single particles in free solution is a key enabling technology for fundamental applied science. Methods particle trapping based on optical, magnetic, electrokinetic, acoustic techniques have led major advancements physics biology ranging from the molecular cellular level. In this article, we introduce new microfluidic-based technique manipulation solely hydrodynamic fluid flow. Using method, demonstrate of micro- nano-scale aqueous solutions long time...

A novel noncontact technique based on hydrodynamic trapping is presented to study the dissolution of freely suspended liquid microdroplets into a second immiscible phase in simple extensional creeping flow. Benzyl benzoate (BB) and n-decanol are individually trapped at stagnation point planar flow, single an aqueous solution containing surfactant characterized different flow rates. The experimental curves compared two models: (i) Epstein-Plesset (EP) model which considers only diffusive mass...

Detection of single bacterial cells through optical resonances has been demonstrated in microdroplets.The setup enables high throughput non-specific detection E. Coli without any labeling.The inside the microdroplet have a direct effect on morphology dependent that are supported by Rhodamine 6G fluorescence; modification arises from changes due to scattering and local refractive index.The change resonance spectrum can be observed at low concentrations where each contains no more than one cell.

Recent advancements in science and engineering have allowed for trapping manipulation of individual particles macromolecules within an aqueous medium using a flow-based confinement method. In this work, we demonstrate the feasibility manipulating two coupled planar extensional flows. Using Brownian dynamics simulations proportional feedback control algorithm, show that micro/nanoscale can be simultaneously confined manipulated at stagnation points pair interconnected We specifically studied...

Fluid-structure interactions (FSI) are used in a variety of sensors based on micro- and nanotechnology to detect measure changes pressure, flow, viscosity fluids. These typically consist flexible structure that deforms response the fluid flow generates an electrical, optical, or mechanical signal can be measured. FSI-based have recently been utilized applications such as biomedical devices, environmental monitoring, aerospace engineering, where accurate measurement properties is critical...

The detection of single bacterial cells and novel absorbing labels has been demonstrated through optical resonances in microdroplets. setup enables high throughput Escherichia coli (E. Coli) without any direct labeling although Rhodamine 6G (R6G) was used as the signal transduction mechanism. A micro droplet acts an cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where circumference is integer multiple emission wavelength. inside have a effect on fluorescence...

Abstract Traditional cultivation approaches in microbiology are labor-intensive, low-throughput, and often yield biased sampling of taxa due to ecological evolutionary factors. New strategies needed enable ample representation rare slow-growers that outcompeted by fast-growing organisms. We developed a microfluidic platform anaerobically isolates cultivates microbial cells millions picoliter droplets automatically sorts based on colony density. applied our strategy mouse human gut...