A5089610178- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
NYU Langone Health
2018-2025
Howard Hughes Medical Institute
2018-2024
New York University
2018-2023
National Taiwan University
2014-2018
Institute of Plant and Microbial Biology, Academia Sinica
2015
University of Essex
2015
Upon recognition of microbe-associated molecular patterns (MAMPs) such as the bacterial flagellin (or derived peptide flg22) by pattern-recognition receptors (PRRs) FLAGELLIN SENSING2 (FLS2), plants activate pattern-triggered immunity (PTI) response. The L-type lectin receptor kinase-VI.2 (LecRK-VI.2) is a positive regulator Arabidopsis thaliana PTI. Cysteine-rich receptor-like kinases (CRKs) possess two copies C-X8-C-X2-C (DUF26) motif in their extracellular domains and are thought to be...
Plasma membrane-localized pattern recognition receptors (PRRs) such as FLAGELLIN SENSING2 (FLS2), EF-TU RECEPTOR (EFR), and CHITIN ELICITOR KINASE1 (CERK1) recognize microbe-associated molecular patterns (MAMPs) to activate pattern-triggered immunity (PTI). A reverse genetics approach on genes responsive the priming agent β-aminobutyric acid (BABA) revealed IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) a critical PTI player. Arabidopsis thaliana ios1 mutants were hypersusceptible Pseudomonas...
The ERF (ethylene responsive factor) family is composed of transcription factors (TFs) that are critical for appropriate Arabidopsis thaliana responses to biotic and abiotic stresses. Here we identified characterized a member the TF group IX, namely ERF96, when overexpressed enhances resistance necrotrophic pathogens such as fungus Botrytis cinerea bacterium Pectobacterium carotovorum. ERF96 jasmonate (JA) ethylene (ET) transcripts accumulation was abolished in JA-insensitive coi1-16...
Abstract CCCTC-binding factor (CTCF) is critical to three-dimensional genome organization. Upon differentiation, CTCF insulates active and repressed genes within Hox gene clusters. We conducted a genome-wide CRISPR knockout (KO) screen identify required for CTCF-boundary activity at the HoxA cluster, complemented by biochemical approaches. Among candidates, we identified Myc-associated zinc-finger protein (MAZ) as cofactor in insulation. MAZ colocalizes with chromatin borders and, similar...
Pattern-triggered immunity (PTI) is broad spectrum and manipulation of PTI believed to represent an attractive way engineer plants with broad-spectrum disease resistance. activated upon perception microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs). We have recently demonstrated that the L-type lectin receptor kinase-VI.2 (LecRK-VI.2) positively regulates Arabidopsis thaliana PTI. Here we show through in vitro pull-down, bimolecular fluorescence...
Partitioning of repressive from actively transcribed chromatin in mammalian cells fosters cell-type-specific gene expression patterns. While this partitioning is reconstructed during differentiation, the occupancy key insulator, CCCTC-binding factor (CTCF), unchanged at developmentally important Hox clusters. Thus, dynamic changes boundaries must entail other activities. Given its requirement for loop formation, we examined cohesin-based without known insulators, CTCF and Myc-associated...
Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates a subset defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset PTI through complex signaling networks. Here, we characterized function ERF19, member Arabidopsis thaliana ethylene response factor (ERF) family. ERF19 was found to act negative regulator against Botrytis cinerea and...
CCCTC-binding factor (CTCF) is essential for chromatin organization. CTCF interacts with endogenous RNAs, and deletion of its ZF1 RNA-binding region (∆ZF1) disrupts loops in mouse embryonic stem cells (ESCs). However, the functional significance CTCF-ZF1 RNA interactions during cell differentiation unknown. Using an ESC-to-neural progenitor (NPC) model, we show that crucial maintaining cell-type-specific loops. Expression CTCF-∆ZF1 leads to disrupted dysregulation genes within these loops,...
Abstract Plasma membrane-localized pattern recognition receptors such as FLAGELLIN SENSING2 (FLS2) and EF-TU RECEPTOR (EFR) recognize microbe-associated molecular patterns (MAMPs) to activate the first layer of plant immunity termed pattern-triggered (PTI). A reverse genetics approach with genes responsive priming agent β-aminobutyric acid (BABA) revealed IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) a critical PTI player. Arabidopsis thaliana ios1 mutants were hypersusceptible Pseudomonas...
SUMMARY Partitioning of repressive from actively transcribed chromatin in mammalian cells fosters cell-type specific gene expression patterns. While this partitioning is reconstructed during differentiation, the occupancy key insulator, CTCF, unchanged at developmentally important Hox clusters. Thus, dynamic changes boundaries must entail other activities. Given its requirement for loop formation, we examined cohesin-based without known insulators, CTCF and MAZ, identified a family zinc...
ABSTRACT Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset PTI through complex signaling networks. Here, we characterize function ERF19, a member Arabidopsis thaliana ethylene response factor (ERF) family. ERF19 was found to act negative regulator against Botrytis cinerea and...
Abstract The essential CCCTC-binding factor (CTCF) is critical to three-dimensional (3D) genome organization. CTCF binding insulates active and repressed genes within the Hox clusters upon differentiation, but such does not participate in boundary formation all cell types, as embryonic stem cells. We conducted a genome-wide CRISPR knockout screen identify required for activity at HoxA cluster, complemented by novel biochemical approaches. This identified Myc-associated zinc finger protein...