A5091616156- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Plant Gene Expression Analysis
- Fungal and yeast genetics research
- Protein Degradation and Inhibitors
- Bioinformatics and Genomic Networks
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Telomeres, Telomerase, and Senescence
Leiden University Medical Center
2016-2025
Novo Nordisk Foundation
2025
University of Copenhagen
2025
Abstract Perturbation of DNA replication, for instance by hydroxyurea-dependent dNTP exhaustion, often leads to stalling or collapse replication forks. This triggers a stress response that stabilizes these forks, activates cell cycle checkpoints, and induces expression damage genes. While several factors are known act in this response, the full repertoire proteins involved remains largely elusive. Here, we develop Replication-IDentifier (Repli-ID), which allows genome-wide identification...
In the process of DNA replication, first steps in restoring chromatin landscape involve parental histone recycling and new deposition. Disrupting to either leading or lagging strand induces asymmetric inheritance, affecting epigenome maintenance cellular identity. However, order kinetics these effects remain elusive. Here, we use inducible mutants dissect early late consequences impaired recycling. Simultaneous disruption both (POLE4) (MCM2-2A) pathways impairs transmission histones newly...
Abstract The collection of known posttranslational modifications (PTMs) has expanded rapidly with the identification various non-acetyl histone lysine acylations, such as crotonylation, succinylation and butyrylation, yet their regulation is still not fully understood. Through an unbiased chromatin immunoprecipitation (ChIP)-based approach called Epigenetics-IDentifier (Epi-ID), we aimed to identify regulators butyrylation in thousands yeast mutants simultaneously. However, highly...